It is important to have a PCR technique capable of detecting avian adenovirus 4 with high sensitivity and precision in cell cultures and samples of avian origin. In the present work, a PCR technique was standardized. First, the Internal Reference Material (IRM) was prepared, using cell culture in EB66 cells, infected with FAdV-4; then the virus was purified, the DNA was extracted and the amplicon was quantified. Finally, the temperature gradient test, primer concentration test, sensitivity test and specificity test were carried out using the Q5® High-Fidelity 2X Master PCR kit. Of the five primers used, the HHS*-3 and HHS*-4 primers passed all the tests, with HHS*-3 being the most sensitive as it detected up to 1 pg/µl.

            It is important to have a PCR technique capable of detecting avian adenovirus 4 with high sensitivity and precision in cell cultures and samples of avian origin. In the present work, a PCR technique was standardized. First, the Internal Reference Material (IRM) was prepared, using cell culture in EB66 cells, infected with FAdV-4; then the virus was purified, the DNA was extracted and the amplicon was quantified. Finally, the temperature gradient test, primer concentration test, sensitivity test and specificity test were carried out using the Q5® High-Fidelity 2X Master PCR kit. Of the five primers used, the HHS*-3 and HHS*-4 primers passed all the tests, with HHS*-3 being the most sensitive as it detected up to 1 pg/µl.

The objective of the work was to optimize duplex PCR for the detection of Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) in tracheal tissue samples in Gallus gallus in order to save time and economic resources in their diagnosis. The methodology consisted of carrying out an in silico evaluation of the ideal primers for their detection, the first tests such as primer concentration, sensitivity and specificity were carried out, followed by determining the prevalence in samples from different poultry houses at national level during a period of 4 years. Once the first tests were completed, the ideal primers (MG 3 and MS 2) were chosen for duplex PCR, achieving a sensitivity and specificity greater than 99%. This technique was put into practice and 669 samples of chicken tracheal tissue were analyzed, in which 45 samples were found to be positive for MG and/or MS. Finally, the pos...

The diseases produced by avian adenovirus Type 4 (FAdV-4) are transmitted vertically and horizontally causing great economic losses in the poultry sector, that is why there is the need to carry out a process of standardisation of a Polymerase chain in reaction (PCR) to detect and quantify our virus of interest. To this end, in the present investigation, it was carried out in three (03) phases: The first consisted in the preparation of the internal reference material where the DNA was extracted, from a culture of EB66 cells, measuring its quantity of the Amplicon through the Fluorometer Qubit 2.0. The second phase was to standardize conventional PCR, in this phase we proceeded to the selection of the specific primers (Primer´s), these were HHS *-2, HHS *-3 and HHS *-4; The test of its temperature gradient was carried out based on the Q5 PCR Kit® High-Fidelity 2X Master, followed by the ...

En la presente investigación se logró determinar la calidad microbiológica de los desayunos ofrecidos por el programa Qali Warma en las instituciones educativas (II.EE) públicas del Bajo Piura. La ejecución de la investigación comprendió tres (03) fases: la primera fue hacer los trámites respectivos con la Dirección Ejecutiva de Salud Ambiental (DESA) para obtener el permiso y poder ejecutar este estudio. La segunda fue realizar un muestreo aleatorio estratificado de las II.EE que son beneficiadas por el programa Qali Warma obteniendo así un tamaño de muestra proporcional para cada distrito muestreado del Bajo Piura (Catacaos, Cura Mori, La Arena y La Unión) y la inspección de lasii.EE con personal de los centros de salud basándose del formulario de inspecciones de almacenes y establecimientos de preparación de desayunos. La tercera fase fue la toma de muestras (superficie...

Las bacterias Mycoplasma gallisepticum (MG) y Mycoplasma synoviae (MS), son los principales patógenos que ocasionan la enfermedad de micoplasmosis aviar a nivel mundial. En el Perú, la mayor cantidad de especies avícolas comerciales pertenecen a la especie Gallus gallus, en donde la propagación de la micoplasmosis aviar ocasionaría grandes pérdidas económicas debido a su rápida propagación, por ello, es necesario detectar dichos patógenos para controlar la infección. La presente investigación consistió en optimizar la detección de ambos patógenos en tres (03) fases. La primera fase consistió en optimizar una PCR para la detección de MG y MS, la segunda fase en la optimización de PCR multiplex para la detección de ambos patógenos en tejidos de Gallus gallus y finalmente en la tercera fase se realizó la secuenciación de las muestras que salieron positivas en las muest...