The objective of the study was to develop a molecular platform for the quantification of Newcastle disease virus (NDV) from a culture system in embryonated SPF eggs. First, four pairs of primers were evaluated that amplify different regions of the NDV viral genome that code for: nucleocapsid protein (NP), protein matrix (M), fusion protein (F) and RNA-dependent RNA polymerase (L) to select the most conserved one from which a molecular platform based on reverse transcription was developed - conventional polymerase chain reaction (RT-PCRc) in two steps for the detection of NDV. Subsequently, it was taken to reverse transcription - real-time polymerase chain reaction (RT-qPCR) for the quantification of NDV produced from a system of embryonated eggs. Through these techniques, it was determined that the primers for the M gene were adequate according to the optimization criteria for the develo...

El estudio tuvo por objetivo desarrollar una plataforma molecular para la cuantificación del virus de la enfermedad de Newcastle (NDV) a partir de un sistema de cultivo en huevos embrionados SPF. Primero se evaluaron cuatro pares de cebadores que amplifican diferentes regiones del genoma viral de NDV que codifican para la proteína de nucleocápside (NP), proteína matriz (M), proteína fusión (F) y la ARN polimerasa ARN dependiente (L), con la finalidad de seleccionar el más conservado a partir del cual se desarrolló una plataforma molecular basada en la transcripción reversa - reacción en cadena de polimerasa convencional (RT-PCRc) en dos pasos para la detección del NDV. Posteriormente, esta fue llevada a transcripción reversa - reacción en cadena de polimerasa en tiempo real (RT-qPCR) para la cuantificación de NDV producido a partir de un sistema de huevos embrionados. Media...

Tourism and hospitality education in Peru is evolving to incorporate a stronger focus on environmental conservation. In this context, this study aims to assess environmental conservation attitudes of students enrolled in tourism and hospitality programs offered by Peruvian universities. A descriptive, cross-sectional study was carried out among students pursuing a degree in Tourism and Hospitality Management at the National University of Cañete, in Lima, Peru. The research focused on the period from July to December 2018. The final report was completed in 2020. A sample of 45 students was taken from a total population of 281, based on specific selection criteria. The demographic variables considered for this study were: age group, gender, and class shift, alongside the following dimensions: cognitive, affective and reactive. The majority of participants were female (73.3%), within the 2...

Tourism and hospitality education in Peru is evolving to incorporate a stronger focus on environmental conservation. In this context, this study aims to assess environmental conservation attitudes of students enrolled in tourism and hospitality programs offered by Peruvian universities. A descriptive, cross-sectional study was carried out among students pursuing a degree in Tourism and Hospitality Management at the National University of Cañete, in Lima, Peru. The research focused on the period from July to December 2018. The final report was completed in 2020. A sample of 45 students was taken from a total population of 281, based on specific selection criteria. The demographic variables considered for this study were: age group, gender, and class shift, alongside the following dimensions: cognitive, affective and reactive. The majority of participants were female (73.3%), within the 2...

Tourism and hospitality education in Peru is evolving to incorporate a stronger focus on environmental conservation. In this context, this study aims to assess environmental conservation attitudes of students enrolled in tourism and hospitality programs offered by Peruvian universities. A descriptive, cross-sectional study was carried out among students pursuing a degree in Tourism and Hospitality Management at the National University of Cañete, in Lima, Peru. The research focused on the period from July to December 2018. The final report was completed in 2020. A sample of 45 students was taken from a total population of 281, based on specific selection criteria. The demographic variables considered for this study were: age group, gender, and class shift, alongside the following dimensions: cognitive, affective and reactive. The majority of participants were female (73.3%), within the 2...

El objetivo general de la tesis es determinar el nivel de actitudes hacia la conservación del ambiente en estudiantes de la Escuela Profesional de Administración del Turismo y Hotelería de la Universidad Nacional de Cañete. Se presenta una investigación de carácter cuantitativa, de tipo básica, nivel descriptivo y diseño no experimental, con una población de 281 estudiantes y una muestra de 45 pertenecientes al quinto año académico, siendo el muestreo no probalistico intencional, para lo cual se diseñó y validó una escala de Tipo Lickert y en el procesamiento de datos se utilizó estadística descriptiva. Tras la aplicación de la encuesta se demostró que el nivel de actitudes favorables hacia la conservación del ambiente de los estudiantes fue alto (73,3%). El componente cognitivo y afectivo tuvieron niveles favorables altos (66,67%), mientras que el componente reactivo ...

Se caracterizó el plasmidio pCTe-01 de Aeromonas veronii CTe-01 aislada de aguas residuales. Esta bacteria es portadora de factores de virulencia y ha mostrado resistencia a metales pesados y a antibióticos, mecanismos pobremente entendidos en este género y atribuidos a la presencia de determinantes genéticos cromosomales y plasmidiales. Para caracterizar el plasmidio pCTe-01 se optimizó su extracción evaluando 4 métodos, además de la realización de los ensayos siguientes: perfil electroforético; determinación del peso molecular, mediante corte con las enzimas restricción BamHI, PstI, BstN y MobI; determinación de la relación entre el plasmidio y la resistencia a metales pesados y antibióticos, mediante experimentos de conjugación bacteriana entre la cepa donadora A. veronii CTe-01 y la cepa receptora E. coli BW25113. Las características de resistencia a metales pesados ...

            It is important to have a PCR technique capable of detecting avian adenovirus 4 with high sensitivity and precision in cell cultures and samples of avian origin. In the present work, a PCR technique was standardized. First, the Internal Reference Material (IRM) was prepared, using cell culture in EB66 cells, infected with FAdV-4; then the virus was purified, the DNA was extracted and the amplicon was quantified. Finally, the temperature gradient test, primer concentration test, sensitivity test and specificity test were carried out using the Q5® High-Fidelity 2X Master PCR kit. Of the five primers used, the HHS*-3 and HHS*-4 primers passed all the tests, with HHS*-3 being the most sensitive as it detected up to 1 pg/µl.

            It is important to have a PCR technique capable of detecting avian adenovirus 4 with high sensitivity and precision in cell cultures and samples of avian origin. In the present work, a PCR technique was standardized. First, the Internal Reference Material (IRM) was prepared, using cell culture in EB66 cells, infected with FAdV-4; then the virus was purified, the DNA was extracted and the amplicon was quantified. Finally, the temperature gradient test, primer concentration test, sensitivity test and specificity test were carried out using the Q5® High-Fidelity 2X Master PCR kit. Of the five primers used, the HHS*-3 and HHS*-4 primers passed all the tests, with HHS*-3 being the most sensitive as it detected up to 1 pg/µl.