En empresas de tecnología que ofrecen el servicio de tercerización de actividades de análisis, desarrollo, pruebas e implementación de proyectos de software resulta fundamental contar con una base de información actualizada de las capacidades técnicas y blandas del personal que brinda el servicio debido a que dicho conocimiento es uno de sus principales activos como organización. En ese contexto, una compañía de software especializada en proyectos de Big Data utilizaba herramientas de propósito general para recopilar y explotar esta información comprometiendo la integridad de los datos y dificultando iniciativas de mejora del servicio. Para solucionar la problemática planteada, se implementó un sistema web que permite al personal operativo de la empresa el registro y actualización de las habilidades técnicas, blandas y certificaciones de TI que poseen actualmente y además...

            It is important to have a PCR technique capable of detecting avian adenovirus 4 with high sensitivity and precision in cell cultures and samples of avian origin. In the present work, a PCR technique was standardized. First, the Internal Reference Material (IRM) was prepared, using cell culture in EB66 cells, infected with FAdV-4; then the virus was purified, the DNA was extracted and the amplicon was quantified. Finally, the temperature gradient test, primer concentration test, sensitivity test and specificity test were carried out using the Q5® High-Fidelity 2X Master PCR kit. Of the five primers used, the HHS*-3 and HHS*-4 primers passed all the tests, with HHS*-3 being the most sensitive as it detected up to 1 pg/µl.

            It is important to have a PCR technique capable of detecting avian adenovirus 4 with high sensitivity and precision in cell cultures and samples of avian origin. In the present work, a PCR technique was standardized. First, the Internal Reference Material (IRM) was prepared, using cell culture in EB66 cells, infected with FAdV-4; then the virus was purified, the DNA was extracted and the amplicon was quantified. Finally, the temperature gradient test, primer concentration test, sensitivity test and specificity test were carried out using the Q5® High-Fidelity 2X Master PCR kit. Of the five primers used, the HHS*-3 and HHS*-4 primers passed all the tests, with HHS*-3 being the most sensitive as it detected up to 1 pg/µl.

The objective of the work was to optimize duplex PCR for the detection of Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) in tracheal tissue samples in Gallus gallus in order to save time and economic resources in their diagnosis. The methodology consisted of carrying out an in silico evaluation of the ideal primers for their detection, the first tests such as primer concentration, sensitivity and specificity were carried out, followed by determining the prevalence in samples from different poultry houses at national level during a period of 4 years. Once the first tests were completed, the ideal primers (MG 3 and MS 2) were chosen for duplex PCR, achieving a sensitivity and specificity greater than 99%. This technique was put into practice and 669 samples of chicken tracheal tissue were analyzed, in which 45 samples were found to be positive for MG and/or MS. Finally, the pos...

The objective of the study was to develop a molecular platform for the quantification of Newcastle disease virus (NDV) from a culture system in embryonated SPF eggs. First, four pairs of primers were evaluated that amplify different regions of the NDV viral genome that code for: nucleocapsid protein (NP), protein matrix (M), fusion protein (F) and RNA-dependent RNA polymerase (L) to select the most conserved one from which a molecular platform based on reverse transcription was developed - conventional polymerase chain reaction (RT-PCRc) in two steps for the detection of NDV. Subsequently, it was taken to reverse transcription - real-time polymerase chain reaction (RT-qPCR) for the quantification of NDV produced from a system of embryonated eggs. Through these techniques, it was determined that the primers for the M gene were adequate according to the optimization criteria for the develo...

El estudio tuvo por objetivo desarrollar una plataforma molecular para la cuantificación del virus de la enfermedad de Newcastle (NDV) a partir de un sistema de cultivo en huevos embrionados SPF. Primero se evaluaron cuatro pares de cebadores que amplifican diferentes regiones del genoma viral de NDV que codifican para la proteína de nucleocápside (NP), proteína matriz (M), proteína fusión (F) y la ARN polimerasa ARN dependiente (L), con la finalidad de seleccionar el más conservado a partir del cual se desarrolló una plataforma molecular basada en la transcripción reversa - reacción en cadena de polimerasa convencional (RT-PCRc) en dos pasos para la detección del NDV. Posteriormente, esta fue llevada a transcripción reversa - reacción en cadena de polimerasa en tiempo real (RT-qPCR) para la cuantificación de NDV producido a partir de un sistema de huevos embrionados. Media...