1
artículo
Publicado 2018
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The objective of the study was to develop a molecular platform for the quantification of Newcastle disease virus (NDV) from a culture system in embryonated SPF eggs. First, four pairs of primers were evaluated that amplify different regions of the NDV viral genome that code for: nucleocapsid protein (NP), protein matrix (M), fusion protein (F) and RNA-dependent RNA polymerase (L) to select the most conserved one from which a molecular platform based on reverse transcription was developed - conventional polymerase chain reaction (RT-PCRc) in two steps for the detection of NDV. Subsequently, it was taken to reverse transcription - real-time polymerase chain reaction (RT-qPCR) for the quantification of NDV produced from a system of embryonated eggs. Through these techniques, it was determined that the primers for the M gene were adequate according to the optimization criteria for the develo...
2
artículo
Publicado 2018
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El estudio tuvo por objetivo desarrollar una plataforma molecular para la cuantificación del virus de la enfermedad de Newcastle (NDV) a partir de un sistema de cultivo en huevos embrionados SPF. Primero se evaluaron cuatro pares de cebadores que amplifican diferentes regiones del genoma viral de NDV que codifican para la proteína de nucleocápside (NP), proteína matriz (M), proteína fusión (F) y la ARN polimerasa ARN dependiente (L), con la finalidad de seleccionar el más conservado a partir del cual se desarrolló una plataforma molecular basada en la transcripción reversa - reacción en cadena de polimerasa convencional (RT-PCRc) en dos pasos para la detección del NDV. Posteriormente, esta fue llevada a transcripción reversa - reacción en cadena de polimerasa en tiempo real (RT-qPCR) para la cuantificación de NDV producido a partir de un sistema de huevos embrionados. Media...
3
Publicado 2022
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Within the framework of the current COVID-19 pandemic, there is a race against time to find therapies for the outbreak to be controlled. Since vaccines are still tedious to develop and partially available for low-income countries, passive immunity based on egg-yolk antibodies (IgY) is presented as a suitable approach to preclude potential death of infected patients, based on its high specificity/avidity/production yield, cost-effective manufacture, and ease of administration. In the present study, IgY antibodies against a recombinant RBD protein of SARS-CoV-2 were produced in specific-pathogen-free chickens and purified from eggs using a biocompatible method. In vitro immunoreactivity was tested, finding high recognition and neutralization values. Safety was also demonstrated prior to efficacy evaluation, in which body weight, kinematics, and histopathological assessments of hamsters cha...
4
artículo
Publicado 2021
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El texto completo de este trabajo no está disponible en el Repositorio Académico UPC por restricciones de la casa editorial donde ha sido publicado.
