Tópicos Sugeridos dentro de su búsqueda.
https://purl.org/pe-repo/ocde/ford#3.01.05 5 Antibacterianos 3 Bactris gasipaes 2 Coliformes 2 E. coli 2 ETA 2 Evaluación 2 más ...
Mostrando 1 - 20 Resultados de 24 Para Buscar '(coli or none)', tiempo de consulta: 2.84s Limitar resultados
1
artículo
The aim if this study was to characterize strains of E. coli isolated from Rattus norvegicus belonging to a level 1 animal facility. Faecal samples were taken from 13 rats. Five colonies were randomly selected from the primary growth of the culture of each sampled individual. Biochemical tests were performed and the pathotype and phylogroup were established in the strains that were identified as E. coli by means of the PCR technique. In addition, tests for biofilm formation and antimicrobial susceptibility were performed. Results showed that 65% (26/40) of the isolated strains corresponded to E. coli and all clustered in phylogroup B2. Six of the E. coli strains were resistant to nitrofurantoin (23%), four were resistant to ampicillin (15%), and three strains were resistant to cefozolin (11%). None showed the formation of biofilms, nor the amplification of genes related to any pathotype....
2
3
artículo
Los genes blaKPC y blaOXA-48 codifican enzimas carbapenemasas en muchas enterobacterias, confiriéndoles resistencia a los antibióticos carbapenémicos, por ello la presente investigación tuvo por objetivo detectar genes blaKPC y blaOXA-48 en cultivos de Escherichia coli y Klebsiella pneumoniae, aislados a partir de urocultivos positivos en el Instituto Regional de Enfermedades Neoplásicas - IREN Norte, Perú. A 100 cultivos bacterianos, se determinó fenotípicamente la presencia de enzimas carbapenemasas, y los cultivos que resultaron positivos a estas pruebas, fueron sometidos a la detección de genes blaKPC y blaOXA-48 en ADN genómico y plasmídico, mediante PCR convencional. Se encontró que el 59 % de los cultivos bacterianos mostraron fenotípicamente producir betalactamasas de espectro extendido (BLEE), además n=6 resultaron ser positivos a carbapenemasas, pero, en la detecc...
4
artículo
Los genes blaKPC y blaOXA-48 codifican enzimas carbapenemasas en muchas enterobacterias, confiriéndoles resistencia a los antibióticos carbapenémicos, por ello la presente investigación tuvo por objetivo detectar genes blaKPC y blaOXA-48 en cultivos de Escherichia coli y Klebsiella pneumoniae, aislados a partir de urocultivos positivos en el Instituto Regional de Enfermedades Neoplásicas - IREN Norte, Perú. A 100 cultivos bacterianos, se determinó fenotípicamente la presencia de enzimas carbapenemasas, y los cultivos que resultaron positivos a estas pruebas, fueron sometidos a la detección de genes blaKPC y blaOXA-48 en ADN genómico y plasmídico, mediante PCR convencional. Se encontró que el 59 % de los cultivos bacterianos mostraron fenotípicamente producir betalactamasas de espectro extendido (BLEE), además n=6 resultaron ser positivos a carbapenemasas, per...
5
artículo
Los genes blaKPC y blaOXA-48 codifican enzimas carbapenemasas en muchas enterobacterias, confiriéndoles resistencia a los antibióticos carbapenémicos, por ello la presente investigación tuvo por objetivo detectar genes blaKPC y blaOXA-48 en cultivos de Escherichia coli y Klebsiella pneumoniae, aislados a partir de urocultivos positivos en el Instituto Regional de Enfermedades Neoplásicas - IREN Norte, Perú. A 100 cultivos bacterianos, se determinó fenotípicamente la presencia de enzimas carbapenemasas, y los cultivos que resultaron positivos a estas pruebas, fueron sometidos a la detección de genes blaKPC y blaOXA-48 en ADN genómico y plasmídico, mediante PCR convencional. Se encontró que el 59 % de los cultivos bacterianos mostraron fenotípicamente producir betalactamasas de espectro extendido (BLEE), además n=6 resultaron ser positivos a carbapenemasas, per...
6
tesis de grado
El excesivo uso irracional de los antibióticos causa que las bacterias desarrollen resistencia frente a éstos, por lo que en el presente trabajo de investigación se efectuó el tamizaje fitoquímico y se determinó la actividad antibacteriana in vitro de la planta entera de Phyllanthus fluitans Benth. Ex Müll. Arg. frente a las cepas bacterianas Staphylococcus aureus ATCC 25923 y Escherichia coli ATCC 25922, para el cual se utilizaron extractos etanólico, diclorometánico y etéreo, a las concentraciones de 26, 30 y 34 mg/mL, 30 y 34 mg/mL, y 34 mg/mL, respectivamente. Para el tamizaje fitoquímico se utilizó el método de Schabra y cols.; para la actividad antibacteriana, el método de Disco Difusión (Kirby-Bauer). La muestra fue recolectada en el rio Itaya ubicado geográficamente a 3°46´11.79´´ Latitud y 73°14´26.89´´ Longitud, luego enviada al HERBARIUM AMAZONENSE de l...
7
tesis de grado
Los metabolitos secundarios que se detectaron en el extracto etanólico de semillas de Carica papaya L. (papaya) fueron los compuestos fenólicos, lactonas α, β-insaturadas, taninos, azúcares reductores, saponinas, antraquinonas, terpenos y esteroides, alcaloides, antocianinas y flavonoides. Por otro lado, mediante la prueba de ANOVA (p<0,05) y Tukey, se demostró como resultado diferencia estadísticamente significativa. La concentración que presentó actividad antibacteriana del extracto etanólico de semillas de Carica papaya L. (Papaya) frente a Staphylococcus aureus ATCC 25923 fue del 50% y el 75%, por otro lado, ninguna de las concentraciones presentó actividad frente Escherichia coli ATCC 25922. Asimismo, ninguna de las concentraciones supero el efecto inhibidor del ciprofloxacino.
8
tesis de grado
En la actualidad, se ha visto un auge de bacterias patógenas resistentes a antibióticos. La aparición de bacterias resistentes a antibióticos no solo de uso regular, pero también resistentes a antibióticos de última línea presenta una seria preocupación de salud a nivel global. Debido a esto, han aumentado los esfuerzos para obtener nuevos compuestos antibióticos. Compuestos ya encontrados en el medio ambiente tienden a ser mejores blancos de estudio, debido a su fácil obtención en comparación con la síntesis de compuestos nuevos. En el presente trabajo se intentó gene rar una bacteria que produjera microcina J25 (MccJ25) en el laboratorio para futuros estudios. Se realizó el cribado de 126 aislados de E. coli patogénicas en búsqueda de los determinantes genéticos de microcina J25 (MccJ25) mediante colony PCR. No se encontraron bacterias que arrojaran un diagnóstico p...
9
artículo
Essential oils are volatile compounds extracted from plants of great importance in the pharmaceutical, food and perfume industries. The research aimed to assess the shelf life of guinea pig meat vacuum packed using Tagetes minuta L. essential oil (AETm). An experimental design ofincreasing stimulus was used, in which three doses of aqueous solution of AE (0.25, 0.30 and 0.35% v / v) were applied, with four replicates and six evaluations were performed in 25 days (O; 3; 6; 14; 18 and 25) storage at 3 º C. The shelf life was determined by microbial load (Escherichia coli and Staphylococus aureus) over time, fat oxidation and pH in 26 g of guinea pig meat. The most probable number (NMP) technique was used to determine the microbial content and the lipid oxidation was determined by Rancimat as the induction time. At 14 days, all samples maintained their characteristics to be consumed; In ad...
10
documento de trabajo
Se ha estudiado la actividad antimicrobiana de extractos etanólicos de propóleo, Apis mellifera, y de películas de quitosano-alcohol polivinílico embebidas en dichas soluciones, frente a S. aureus, E. coli y P. aeruginosa. El extracto etanólico de propóleo presenta actividad antimicrobiana frente a S. aureus ATCC 25923, inclusive cuando se le diluye en una solución hidroalcohólica al 10 %. En cambio, las películas de quitosano-PVA embebidas en la misma solución hidroalcohólica de propóleo no presentan actividad antimicrobiana frente a la misma cepa. Ninguna de las muestra ensayadas presentó actividad frente a E. coli y P. aeruginosa.
11
artículo
Recently, artisanal masses of pilar corn have become a food for mass consumption in Venezuela, without adequate sanitary control in terms of production and sale. There are very few internationally referenced studies and none recently locally, regarding this product; for this reason, the objective of this research has focused on its microbiological evaluation with samples obtained in the municipal market of Puerto La Cruz, which are the main point of sale of the same in the main Venezuelan cities. The frequencies of total coliforms, fecal coliforms, presence of Escherichia coli, aerobic mesophiles and Staphylococcus aureus were determined as indicators of inadequate manipulation; The presence of molds and yeasts was also considered as microorganisms associated with cereals. It is concluded that all the samples of pillared corn masses present a high level of these indicator microorganisms ...
12
artículo
Recently, artisanal masses of pilar corn have become a food for mass consumption in Venezuela, without adequate sanitary control in terms of production and sale. There are very few internationally referenced studies and none recently locally, regarding this product; for this reason, the objective of this research has focused on its microbiological evaluation with samples obtained in the municipal market of Puerto La Cruz, which are the main point of sale of the same in the main Venezuelan cities. The frequencies of total coliforms, fecal coliforms, presence of Escherichia coli, aerobic mesophiles and Staphylococcus aureus were determined as indicators of inadequate manipulation; The presence of molds and yeasts was also considered as microorganisms associated with cereals. It is concluded that all the samples of pillared corn masses present a high level of these indicator microorganisms ...
13
artículo
En este trabajo se evaluaron las propiedades bioactivas antibacterianas y antimicóticas de 33 extractos (etanol, diclorometano, hexano) obtenidos de 11 especies de algas marinas recolectadas en las localidades de San Juan de Los Cayos y Chichiriviche, Estado Falcón, Venezuela. La actividad antibiótica y antimicótica de los extractos se evaluó mediante la aparición de halos de inhibición contra bacterias Gram positivas (Staphylococ- cus aureus), Gram negativas (Pseudomona aeruginosa, Klebsiella pneumoniae, Escherichia coli) y el hongo Candida albicans. De los 33 extractos ensayados sólo 17 presentaron actividad antibacteriana (5 con etanol, 6 con diclorometano y 6 con hexano), resultando activos 14 frente a las especies Gram(-) y 4 contra la especie Gram(+). Las especies algales que mostraron actividad antibacteriana fueron: Acanthophora sp., Bryothamnion triquetrum, Gracilaria sp...
14
artículo
This study assessed the antibacterial and antifungal properties of 33 extracts (ethanol, dichloromethane, hexane) from 11 species of marine algae collected in the villages of San Juan de Los Cayos and Chichiriviche, Estado Falcon, Venezuela. The antibiotics and antifungal activity of extracts was evaluated by the appearance of halos of inhibition against gram-positive bacteria (Staphylococcus aureus), Gram negative (Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli) and the fungus Candida albicans. Of the 33 tested extracts showed antibacterial activity only 17 (5 with ethanol, 6 and 6 with dichloromethane-hexane), resulting assets compared to 14 species Gram(-) and 4 against the kind Gram(+). The algae species that showed antibacterial activity were: Acanthophora sp., Bryothamnion triquetrum, Gracilaria sp., Gelidium sp., Caulerpa mexicana, Caulerpa sp., Caulerpa spp., Hal...
15
tesis de grado
The study determined the in vitro antibacterial activity of ethanolic extracts of leaves and fruits of Couroupita guianensis and Couroupita subsessilis against pathogenic bacteria from Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 15442, Klebsiella pneumoniae ATCC 13883 and Salmonella typhimurium ATCC 14028. The study had a quantitative-experimental approach that included phytochemical screening and bacterial sensitivity tests using Kirby-Bauer disk diffusion and minimum inhibitory concentration (MIC) methods. Phytochemical screening resulted in both plant species showing abundance of carotenes, alkaloids and saponins. The results showed that the leaf extract of C. guianensis presented inhibition halos of 7 mm to 7.5 mm on S. aureus, while C. subsessilis leaf extract showed inhibition halos of 7.3 mm to 10.7 mm for E. coli; and the fruit extra...
16
artículo
The aim of this study was to evaluate the effects of the addition of natural yoghurt, whey and molasses in liquid silages of peach palm (Bactris gasipaes) on physicochemical, biological and organoleptic indicators for use in animal feed. Three silages were used using the mature drupe of the plant: peach palm with natural yogurt (CYN), peach palm with whey (CSL), and peach palm with milk whey and molasses (CSLM). Thirty microsilos were prepared per treatment of 1 kg each, which were evaluated on days 1, 4, 8, 15, 30 and 60 of the start of the conservation process (five per treatment). The variables studied were temperature, pH, presence of bacteria (E. coli, Clostridium spp, Salmonella spp), smell, color and consistency. Higher temperatures were found in CYN (22.56 °C) and CSLM (22.52 °C) on day 1, CYN (22.48 °C) and CSL (22.42 °C) on day 15, CYN (22.50 °C) on day 30, and CYN (22.46 ...
17
artículo
The aim of this study was to evaluate the effects of the addition of natural yoghurt, whey and molasses in liquid silages of peach palm (Bactris gasipaes) on physicochemical, biological and organoleptic indicators for use in animal feed. Three silages were used using the mature drupe of the plant: peach palm with natural yogurt (CYN), peach palm with whey (CSL), and peach palm with milk whey and molasses (CSLM). Thirty microsilos were prepared per treatment of 1 kg each, which were evaluated on days 1, 4, 8, 15, 30 and 60 of the start of the conservation process (five per treatment). The variables studied were temperature, pH, presence of bacteria (E. coli, Clostridium spp, Salmonella spp), smell, color and consistency. Higher temperatures were found in CYN (22.56 °C) and CSLM (22.52 °C) on day 1, CYN (22.48 °C) and CSL (22.42 °C) on day 15, CYN (22.50 °C) on day 30, and CYN (22.46 ...
18
artículo
The Amazon region is one of the areas with the largest biodiversity in the world, hosting several thousand species of plants, many of which are used by its people as medicinal plants. In recent years, the use of these plant resources or products has been increasing significantly, which could be due to a number of factors, among them the knowledge of their chemical composition, and the fact that at present there have been numerous pharmacological tests both in vivo and in vitro. The emergence of antibiotic-resistant strains in recent years, is creating a need for other strategies or ways to control them, as in the case of the use of plants (traditional medicine), because the active ingredients bearing. This work is intended to determine probable alternatives to combat bacterial infections of those agents antibiotic-resistant, this problem is particularly critical in developing countries, ...
19
artículo
The goal of this study is to better understand the complexity of the canonical Wnt/β-catenin (C-WNT) signaling pathway in colorectal cancer (CRC): how it works, its key mutations and the novel therapeutic compounds under development. PubMed, Scopus and SciELO were used to find the most relevant medical literature on the issue. The C-WNT signaling pathway regulates essential cellular biological processes: genetic changes in this pathway are significant contributors to CRC. The Wnt ligand binding to the Frizzled receptor and low-density lipoprotein receptor-related protein 5 or 6 (LRP5/6) stimulates the C-WNT signaling pathway; this extracellular membrane ternary complex activates kinases that promote phosphorylation of the intracellular domain of LRP5/6 and starts the cell signaling cascade. The C-WNT signaling pathway is changed in more than 90 % of all CRCs, with the adenomatous polypo...
20
tesis de grado
Bacterial resistance is a public health problem, which is why it has become a global threat, causing infections caused by resistant bacteria to become increasingly deadly. The objective of this research work was to carry out phytochemical screening and determine the antibacterial activity of the aqueous, ethanolic and trichloromethane extracts of leaves and root of P. obtusilimbum C.DC. and P. divaricatum G. Mey. against Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853 and Escherichia coli ATCC 25922 and their clinical isolates. The following methods were used: a) Phytochemical screening, and b) Kirby-Bauer Disk Diffusion Method. Results: In the phytochemical screening of the ethanolic and trichloromethane extract of the leaves and roots of both species, an abundance of: triterpenes and steroids, carotenes, phenols and tannins, lactones, cardiotonic glycosides, flavono...