Tópicos Sugeridos dentro de su búsqueda.
Tópicos Sugeridos dentro de su búsqueda.
1
artículo
Publicado 2018
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This study focuses on the descendants of the royal Inka family. The Inkas ruled Tawantinsuyu, the largest pre-Columbian empire in South America, which extended from southern Colombia to central Chile. The origin of the royal Inkas is currently unknown. While the mummies of the Inka rulers could have been informative, most were destroyed by Spaniards and the few remaining disappeared without a trace. Moreover, no genetic studies have been conducted on present-day descendants of the Inka rulers. In the present study, we analysed uniparental DNA markers in 18 individuals predominantly from the districts of San Sebastian and San Jerónimo in Cusco (Peru), who belong to 12 families of putative patrilineal descent of Inka rulers, according to documented registries. We used single-nucleotide polymorphisms and short tandem repeat (STR) markers of the Y chromosome (Y-STRs), as well as mitochondri...
2
artículo
Publicado 2021
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Sheath blight caused by Rhizotonia solani is described as the second major disease affecting rice. Genetic resistance to R. solani the ideal control measure is hampered because of the difficulty in identifying adequate resistance sources under typical selection conditions. Proteomic analysis techniques using two-dimensional gels (2D-PAGE) allow the study or monitoring of global changes in protein expression under normal and stress conditions. In this work, we compared rice leaves protein expression patterns of two Venezuelan varieties 12, 24 and 48 h after inoculation with R. solani. Approximately 400 and 300 protein spots stained with Sypro Ruby were reproducibly resolved across gel replicates, for PALMAR and FONAIAP-2000, respectively. Forty proteins out of a total 49 were identified for PALMAR variety, with thirty-two up-regulated protein spots and 8 down-regulated. Twenty-six protein...
3
artículo
Publicado 2024
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El virus del síndrome de las manchas blancas (VSMB), es uno de los principales patógenos de langostinos peneidos reportado a nivel mundial. Desde su primera aparición hasta la actualidad, el VSMB ha mostrado una notable capacidad para alterar su estructura genética, lo que ha dado lugar a diversidad de genotipos. Con el objetivo de detectar las variantes genotípicas del VSMB presentes en el cultivo de langostinos de la costa norte del Perú (Región Tumbes), en esta investigación, se evaluaron 89 muestras de branquias y postlarvas de langostinos infectados con el VSMB que fueron colectados entre los años 2014 y 2021. El análisis fue realizado mediante la PCR (reacción en cadena de la polimerasa) y secuenciamiento de regiones variables del ADN, ubicados en los marcos de lectura abierta (ORFs= open reading frame) 75, 94, 125, 14/15, 23/24 y un gen transposasa presuntiva. En compar...
4
artículo
Publicado 2020
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Microalgae as photosynthetic microorganisms are the source of valuable compounds such as proteins, carotenoids, lipids and carbohydrate polymers. Among the different phyla, Porphyridium as part of the red microalgae (Rhodophyta) are of high importance as producers of sulfated polysaccharides. As the name suggests these microalgae are typically red; however, Porphyridium sordidum has an olive-green color and was recently described as a putative exopolysaccharide producer. Exopolysaccharides (EPS) are sugars polymers excrete to the culture medium. In this study P. sordidum is evaluated for the first time in detail as newly described EPS producer in direct comparison to the already characterized EPS producer Porphyridium purpureum. The evaluation was performed on several aspects, such as morphological differences between the two strains followed by the comparison of the growth behavior, nit...
5
artículo
Publicado 2017
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Here, we report the complete genome sequence of a high alkaline cellulase producing Aspergillus fumigatus strain LMB-35Aa isolated from soil of Peruvian Amazon rainforest. The genome is ∼27.5 mb in size, comprises of 228 scaffolds with an average GC content of 50%, and is predicted to contain a total of 8660 protein-coding genes. Of which, 6156 are with known function; it codes for 607 putative CAZymes families potentially involved in carbohydrate metabolism. Several important cellulose degrading genes, such as endoglucanase A, endoglucanase B, endoglucanase D and beta-glucosidase, are also identified. The genome of A. fumigatus strain LMB-35Aa represents the first whole sequenced genome of non-clinical, high cellulase producing A. fumigatus strain isolated from forest soil.
6
artículo
Publicado 2021
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The fruit of Vanilla planifolia is broadly preferred by the agroindustry and gourmet markets due to its refined flavor and aroma. Peruvian Vanilla has been proposed as a possible source for genetic improvement of existing Vanilla cultivars, but, little has been done to facilitate comprehensive studies of these and other Vanilla. Here, a nuclear magnetic resonance (NMR) metabolomic platform was developed to profile for the first time the leaves – organ known to accumulate vanillin putative precursors – of V. planifolia and those of Peruvian V. pompona, V. palmarum, and V. ribeiroi, with the aim to determine metabolic differences among them. Analysis of the NMR spectra allowed the identification of thirty-six metabolites, twenty-five of which were quantified. One-way ANOVA and post-hoc Tukey test revealed that these metabolites changed significantly among species, whilst multivariate-a...
7
artículo
Publicado 2010
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A. Lopez-Lopez, M. A. Rogel-Hernandez, Monica Rosenblueth, and L. Raymundo are thanked for technical assistance. This research was supported by Consejo Nacional de Ciencia y Tecnologia (Concytec), Integrated Crop Management Division of International Potato Center (CIP), FDA biol-111/UNALM, DGAPA-PAPIIT IN200709 project, and Red Biofag-Cyted. We are grateful to Dr. Andreas Oswald (CIP) for his support in the collection of samples.
8
artículo
Publicado 2018
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This work was funded by Concejo Nacional de Ciencias y Tecnología (CONCYTEC)—Peru. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript
9
artículo
Publicado 2017
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The microbiome of three different sites at the Peruvian Pacific coast was analyzed, revealing a lower bacterial biodiversity at Isla Foca than at Paracas and Manglares, with 89 bacterial genera identified, as compared to 195 and 173 genera, respectively. Only 47 of the bacterial genera identified were common to all three sites. In order to obtain promising strains for the putative production of novel antimicrobials, predatory bacteria were isolated from these sampling sites, using two different bait organisms. Even though the proportion of predatory bacteria was only around 0.5% in the here investigated environmental microbiomes, by this approach in total 138 bacterial strains were isolated as axenic culture. 25% of strains showed antibacterial activity, thereby nine revealed activity against clinically relevant methicillin resistant Staphylococcus aureus (MRSA) and three against enteroh...
10
artículo
Publicado 2008
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The present article is about the possession within the frame of the Peruvian civil right and focuses its attention on the possession and the roll of the title in the exercise of this real right. The right of posesion is approached from the point of view of the legal act and its effect as a cause of the possessory right, as well as the right that the law recognizes to the possessor of the good. Putative title and fair title, interversion of the title of possession, mediate and immediate possession, legitimate and illegitimate possession, precarious possession constitute subjects that are disussed in this article.
11
artículo
Publicado 2008
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The present article is about the possession within the frame of the Peruvian civil right and focuses its attention on the possession and the roll of the title in the exercise of this real right. The right of posesion is approached from the point of view of the legal act and its effect as a cause of the possessory right, as well as the right that the law recognizes to the possessor of the good. Putative title and fair title, interversion of the title of possession, mediate and immediate possession, legitimate and illegitimate possession, precarious possession constitute subjects that are disussed in this article.
12
artículo
Publicado 2019
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RESUMEN El inicio de la era genómica y la invención de técnicas moleculares vanguardistas han revolucionado la genética en el transcurso de las últimas décadas. Estos avances científicos han permitido revelar mecanismos desconocidos que controlan la diferenciación celular y el desarrollo de estructuras novedosas y originales en diversos organismos a lo largo de su historia evolutiva. Una de dichas estructuras es el sistema nervioso, el cual está presente en todos los animales excepto en esponjas y placozoos. La aparición de esta estructura significó un gran salto evolutivo dentro del filo Metazoo, y su amplia diversificación en cada grupo animal ha sido materia de debate en los últimos años. En este artículo, se discuten los posibles orígenes del sistema nervioso. Palabras clave: Bilaterales, células neurales, ctenóforos, metazoos, sistema nervioso. ABSTRACT The...
13
artículo
Publicado 2019
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RESUMEN El inicio de la era genómica y la invención de técnicas moleculares vanguardistas han revolucionado la genética en el transcurso de las últimas décadas. Estos avances científicos han permitido revelar mecanismos desconocidos que controlan la diferenciación celular y el desarrollo de estructuras novedosas y originales en diversos organismos a lo largo de su historia evolutiva. Una de dichas estructuras es el sistema nervioso, el cual está presente en todos los animales excepto en esponjas y placozoos. La aparición de esta estructura significó un gran salto evolutivo dentro del filo Metazoo, y su amplia diversificación en cada grupo animal ha sido materia de debate en los últimos años. En este artículo, se discuten los posibles orígenes del sistema nervioso. Palabras clave: Bilaterales, células neurales, ctenóforos, metazoos, sistema nervioso. ABSTRACT The...
14
artículo
Publicado 2009
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Batrachophrynus y Telmatobius son los dos únicos géneros reconocidos de Telmatobiinae presentes en los Andes centrales. Las especies de ambos géneros presentan adaptaciones para la vida en la altitud de los Andes siendo de hábitos acuáticos o semiacuáticos en bofedales, riachuelos, lagunas o lagos altoandinos. Este estudio presenta las relaciones filogenéticas entre Batrachophrynus y 13 especies de Telmatobius utilizando caracteres morfológicos larvales y adultos, incluyendo caracteres diagnósticos para Batrachophrynus y Telmatobius, y las sinapomorfías sugeridas para Telmatobius. El análisis filogenético dio como resultado 20 árboles igualmente parsimoniosos con una longitud de 56 pasos. Batrachophrynus forma un grupo monofilético anidado dentro del clado de Telmatobius. En este estudio, la mayoría de sinapomorfías que sustentan a Telmatobius (incluyendo a Batrachophrynu...
15
artículo
Publicado 2009
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Batrachophrynus and Telmatobius are the two genus of Telmatobiinae from the central Andes. Both genera have species with adaptations for life at high altitude in the Andes, with aquatic or semi-aquatic habits in creeks, lagoons and lakes. The objective of this study is to evaluate the phylogenetic relationships between Batrachophrynus and 13 species of Telmatobius from the central Andes using larval and adult morphology including diagnostic characters for Batrachophrynus and Telmatobius, and putative sinapomorphies for Telmatobius. The phylogenetic analysis showed 20 parsimonious trees with 56 steps length. The results of this study hypothesize that the species assigned to Batrachophrynus form a monophyletic group nested within Telmatobius. In this study, most of the synapomorphies that support Telmatobius (including Batrachophrynus) come from larval morphology and these sinapomorphies w...
16
artículo
Publicado 2008
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En el presente trabajo se describe una serie de procedimientos bioinformáticos para la predicción de un grupo secuencias ortólogas conservadas (COS) de Ipomoea batatas, así como la evaluación de su potencial utilidad para la generación de marcadores moleculares y estudios de diversidad en esta especie. Con ese propósito usando los programas BLAST X y TBLASTN se realizó una comparación reciproca por similaridad entre secuencias ESTs procedentes de librerías de cDNAs de Ipomoea batatas, propias o disponibles de modo público en la base de datos GenBank, con secuencias COS de Arabidopsis thaliana. La anotación funcional de las secuencias COS predichas en Ipomoea batatas se realizo usando los programas BLASTX, INTERPROSCAN y PSI-BLAST. Se obtuvieron en total 204 secuencias COS candidatos de Ipomoea batatas, siendo 16 secuencias provenientes de una librería generada a partir de ra...
17
artículo
Publicado 2024
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The Saki monkeys, genus Pithecia Desmarest, 1804, have undergone drastic taxonomic changes in a 2014 revision of the genus. In this revision, Pithecia hirsuta Spix, 1823 and P. inusta Spix, 1823 were considered as valid, although both species have been widely considered synonymous with P. monachus. Nonetheless, here we demonstrate, by reviewing the original descriptions of both species, that P. inusta cannot be applied to the Peruvian populations of Pithecia inhabiting primarily the Ucayali River watershed since the original concept of this species, as well as its type locality did not include its putative geographic range. In fact, the area falls completely outside the expedition's itinerary that collected the holotype. Thus, to preserve stability until further evidence is furnished, the above-mentioned Pithecia populations should be regarded as P. monachus.
18
artículo
Publicado 2008
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We develop some bioinformatics procedures to predict Conserved Ortholog Set (COS) sequences from Ipomoea batatas, and evaluate their usefulness for molecular markers and diversity studies of this species. We predict orthology relationship between Ipomoea batatas ESTs sequences and Arabidopsis thaliana COS sequences, according to Best Bidirectional Hits (BBHs) criteria, realizing similarity comparison using BLAST X and TBLASTN programs. We obtained a set of 204 putative COS sequences, 16 of them belonged to storage roots. Functional annotation of sweet potato predicted sequences COS was realized using BLASTX, INTERPROSCAN and PSI-BLAST programs. We evaluate possible polymorphisms in COS candidate sequences, finding SNPs in eight sequences and tandem repeats in one of them.
19
artículo
Publicado 2024
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The Saki monkeys, genus Pithecia Desmarest, 1804, have undergone drastic taxonomic changes in a 2014 revision of the genus. In this revision, Pithecia hirsuta Spix, 1823 and P. inusta Spix, 1823 were considered as valid, although both species have been widely considered synonymous with P. monachus. Nonetheless, here we demonstrate, by reviewing the original descriptions of both species, that P. inusta cannot be applied to the Peruvian populations of Pithecia inhabiting primarily the Ucayali River watershed since the original concept of this species, as well as its type locality did not include its putative geographic range. In fact, the area falls completely outside the expedition's itinerary that collected the holotype. Thus, to preserve stability until further evidence is furnished, the above-mentioned Pithecia populations should be regarded as P. monachus.
20
artículo
Publicado 2020
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Western South America was one of the worldwide cradles of civilization. The well-known Inca Empire was the tip of the iceberg of an evolutionary process that started 11,000 to 14,000 years ago. Genetic data from 18 Peruvian populations reveal the following: 1) The between-population homogenization of the central southern Andes and its differentiation with respect to Amazonian populations of similar latitudes do not extend northward. Instead, longitudinal gene flow between the northern coast of Peru, Andes, and Amazonia accompanied cultural and socioeconomic interactions revealed by archeology. This pattern recapitulates the environmental and cultural differentiation between the fertile north, where altitudes are lower, and the arid south, where the Andes are higher, acting as a genetic barrier between the sharply different environments of the Andes and Amazonia. 2) The genetic homogeniza...
