Bostryx is among the most diverse genera within the Orthalicidae (Gastropoda). Bostryx conspersus is endemic of the Lomas ecosystem in Peruvian Coastal Desert. In this paper, chromosomes in prophase I of meiosis of spermatocytes Bostryx conspersus are described.

Bostryx es uno de los géneros de mayor diversidad dentro de los Orthalicidae (Gastropoda), siendo B. conspersus una especie endémica del ecosistema de Lomas, del desierto Costero peruano. En el presente trabajo los cromosomas de la profase I de meiosis de los espermatocitos de Bostryx conspersus son descritos.

Physalis peruviana (Golden berry) a native fruit of Peru, is an important Andean agricultural crop. Although classical cytogenetics research on this berry has been conducted there is no precise evidence based on molecular cytogenetic studies. The Fluorescent In Situ Hybridization (FISH) technique was applied to reveal 5S rDNA sites in chromosomes of San Pablo and Celendín populations, Cajamarca, Perú. This research was identified the 5S rDNA locus in the two populations of Golden berry, reporting six signals in 3 pairs of chromosomes which would indicate that the species is polyploid. Likewise, no differences were found in the number of 5S rDNA loci between both populations, so we could point out that they would not be different ecotypes.

Physalis peruviana (Golden berry) a native fruit of Peru, is an important Andean agricultural crop. Although classical cytogenetics research on this berry has been conducted there is no precise evidence based on molecular cytogenetic studies. The Fluorescent In Situ Hybridization (FISH) technique was applied to reveal 5S rDNA sites in chromosomes of San Pablo and Celendín populations, Cajamarca, Perú. This research was identified the 5S rDNA locus in the two populations of Golden berry, reporting six signals in 3 pairs of chromosomes which would indicate that the species is polyploid. Likewise, no differences were found in the number of 5S rDNA loci between both populations, so we could point out that they would not be different ecotypes.

Physalis peruviana, fruto nativo del Perú, conocida también como aguaymanto, capulí, uchuva, uvilla, ushun, entre otros; es una planta perteneciente a la familia Solanaceae cuyo centro de origen y diversificación se encuentra en los países andinos de Perú, Colombia y Ecuador, principalmente. Actualmente es de gran importancia económica en la agricultura andina por su contenido farmacológico y nutricional. En el Perú hay pocos estudios a nivel de citogenética básica no existiendo información detallada a nivel de citogenética molecular; para contribuir con información pertinente, el presente estudio identifica regiones 5s rADN utilizando la técnica de Hibridación Fluorescente In Situ – FISH, en los cromosomas de poblaciones cultivadas de San Pablo y Celendín, Cajamarca, Perú. Se han identificado 6 señales en 4 pares de cromosomas hibridados no encontrándose diferencia...

In this work, we characterized cytogenetically Physalis peruviana “aguaymanto” and cultivated populations of the Cajamarca region: San Pablo, Celendín and Cajabamba are compared. The most frequent chromosomal number in the three populations was 2n = 4x = 48 with frequencies of 60, 50 and 34% in San Pablo, Celendín and Cajabamba respectively. Few cases of somatic aneuploidy were found. Our results let distinguish the populations by its karyotypic formula 17m + 4sm + 3t, ​​24m and 20m + 2sm + 2t (San Pablo, Celendín and Cajabamba respectively). Karyotypes were characterized by the total length of the haploid complement (HCL) and the indices of symmetry (S%), asymmetry (A) and intra and interchromosomal asymmetry (A1 and A2). The highest value of HCL was described in San Pablo. Celendín presented the highest degree of symmetry (S% = 53.226 and A = 0.177), while the karyotypes of...

En este trabajo se caracteriza y compara citogenéticamente Physalis peruviana “aguaymanto” de poblaciones cultivadas de la región Cajamarca: San Pablo, Celendín y Cajabamba. El número cromosómico más frecuente en las tres poblaciones fue 2n = 4x = 48 con frecuencias de 60, 50 y 34% en las poblaciones de San Pablo, Celendín y Cajabamba respectivamente. En menor frecuencia se encontró casos de aneuploidía somática. Los resultados permitieron distinguir cada población a partir de su fórmula cariotípica 17m+4sm+3t, 24m y 20m+2sm+2t que identifican a San Pablo, Celendín y Cajabamba respectivamente. Los cariotipos fueron caracterizados por la longitud total del complemento haploide (HCL) y los índices de simetría (S%), asimetría (A) y asimetría intra e intercromosómica (A1 y A2). El mayor valor de HCL se describió en San Pablo. Celendín presentó el mayor grado de sime...

Physalis peruviana L. "Aguaymanto" es una planta oriunda del Perú con presuntas propiedades anticancerígenas. El obetivo del estudio fue evaluar el potencial antiproliferativo del aguaymanto sobre líneas celulares de leucemia mieloide crónica (K562) y fibroblastos de riñón de mono (VERO). A los cultivos se les añadieron los tratamientos de aguaymanto (50, 100, 200 y 400 ug/ml) y cisplatino (0.3, 0.6, 1.25 y 2.5 ug/ml). Se analizó el poder antiproliferativo mediante viabilidad celular (conteo celular y ensayo MTT, Kruskall-Wallis, p<0.05) y la apoptosis con genotoicidad celular mediante el promedio de ncleos daados (ensayo cometa, ANOVA, p<0.05). Los resultados mostraron ue el potencial antiproliferativo y genotóxico fueron dosis-dependientes a la concentración del tratamiento y que el aguaymanto mostró mayor efecto menor viabilidad celular y más núcleos dañados en la ...

Physalis peruviana L. "Aguaymanto´" es una planta oriunda del Perú con presuntas propiedades anticancertgenas. El objetivo del estudio fue evaluar el potencial antiproliferativo del aguaymanto sobre líneas celulares de leucemia mieloide crónica (K562) y fibroblastos de rixón de mono (VE52).  A los cultivos se les añadieron los tratamientos de aguaymanto (50, 100, 200 y 400 ug/ml) y  cisplatino (0.3, 0.6, 1.25 y 2.5 ug/ml). Se analizó el poder antiproliferativo mediante viabilidad celular (conteo celular y ensayo M77, Kruskall-:allis, p<0.05) y la apoptosis con genotoxicidad celular mediante el promedio de núcleos daxados (ensayo cometa, AN2VA, p< 0.05). Los resultados mostraron que el potencial antiproliferativo y genotóxico fueron dosis-dependientes a la concentración del tratamiento y que el aguaymanto mostró mayor efecto (menor viabilidad celular y mis núcl...

This study evaluates the genotoxic injury in the species Colossoma macropomum (“Gamitana") when exposed to the pesticide Fipronil, for which we used the micronucleus test (MN). We workwed with juveniles in the third stage, placed in glass tanks, which were exposed to 3 different concentrations of fipronil (C1: 0.075 mg /L, C2: 0.15 mg /L and C3: 0.30 mg /L), evaluated at 24 and 48 h. Peripheral blood was used which made the spread. The counting of micronuclei and abnormalities was made on the basis of 1000 cells. The average rate of micronuclei indicates that 0.075 mg / L the average frequency is twice the control. With regard to abnormalities of the core, was found more frequently at 48 h of exposure.

This study evaluates the genotoxic injury in the species Colossoma macropomum (“Gamitana") when exposed to the pesticide Fipronil, for which we used the micronucleus test (MN). We workwed with juveniles in the third stage, placed in glass tanks, which were exposed to 3 different concentrations of fipronil (C1: 0.075 mg /L, C2: 0.15 mg /L and C3: 0.30 mg /L), evaluated at 24 and 48 h. Peripheral blood was used which made the spread. The counting of micronuclei and abnormalities was made on the basis of 1000 cells. The average rate of micronuclei indicates that 0.075 mg / L the average frequency is twice the control. With regard to abnormalities of the core, was found more frequently at 48 h of exposure.

Somatic chromosomes of Caesalpinia spinosa (Feuillée ex Molina) Kuntze, “Tara”, wild populations of Huinco and Palca (Junín) regions were studied. The specie were diploid (2n=24). Chromosomes were small. The karyotypes showed the same chromosome number, they found differences in morphological parameters of the same, with the karyotype formula for the town of Huinco: 6m + 6 sm and the town of Palca: 5m + 7 sm.

Somatic chromosomes of Caesalpinia spinosa (Feuillée ex Molina) Kuntze, “Tara”, wild populations of Huinco and Palca (Junín) regions were studied. The specie were diploid (2n=24). Chromosomes were small. The karyotypes showed the same chromosome number, they found differences in morphological parameters of the same, with the karyotype formula for the town of Huinco: 6m + 6 sm and the town of Palca: 5m + 7 sm.