ABSTRACT The present investigation has as purpose production of biofloculant dextran using Leuconostoc mesenteroides var mesenteroides isolated from residual cane juice, as well as, to evaluate its in vitro flocculant activity in effluents from la azucarera Cartavio S.A.A. Leuconostoc mesenteroides var mesenteroides was isolated from 15 samples of residual cane juice from the fields of Casa Grande S.A.A. for which 0.1 ml of juice of the dilution of 105 was inoculated in MSE agar selective medium (Mayeux, Sandine & Elliker) and incubated at 30 ° C for 48 hours, at pH 7.2. The bacteria were isolated by obtaining four biochemically identified strains according to the Bergey’s Manual of Bacteriological Determination. The strain encoded as LM03 was identified as L mesenteroides var mesenteroides producing the best dextran production results with minimum values of 2.61 g / L and maximum of ...

Power generation and wastewater treatment are two great challenges for sustainable development. Microbial fuel cells (MFCs) are a sustainable alternative that can generate bioelectricity in the bioremediation process of wastewater. For this reason, the objective of this research was to generate bioelectricity through double-chamber microbial-combustion cell systems from wastewater from the Covicorti Wastewater Treatment Plant (PTARC) in the anodic chamber and electrogenic bacteria such as Stenotrophomonas maltophilia, Acinetobacter bereziniae, and Achromobacteria xylosoxidans in the cathode chamber, respectively. Measurements of the voltage, current, power density, current density, and optical density of the bacteria and biochemical oxygen demand (BOD) were made. In addition, a metagenomic analysis of the wastewater sample was performed. It was shown that the MFC with A. xylosoxidans gen...

Antibiotics are often prescribed to treat infections caused by group B Streptococcus; however, inappropriate use of antibiotics can develop resistance. Because of this, the research was carried out with the aim of evaluating the in vitro effect of the hydroalcoholic extract of Caesalpinia spinosa (Molina) Kuntze known as Taya or Tara on the viability of β-hemolytic streptococci; an experimental investigation of increasing stimulation was carried out. The hydroalcoholic extract of C. spinosa pods was worked in concentrations of 250, 500, 750, and 1000 mg/mL, which were placed on filter paper discs to perform the sensitivity test following the Kirby–Bauer method. The greatest inhibition of bacterial viability was observed in the penicillin control group (GPT-01) followed by the TCT-04 group (hydroalcoholic Tara extract 1000 mg/mL). In addition, it was found that these groups are statist...

Microorganisms’ degradation of agro-industrial waste produces bad odors and greenhouse gases that contribute to global warming. Consequently, eco-friendly, sustainable biotechnological alternatives to this waste are sought to provide additional value, which is why this study’s objective was to develop a method of producing unicellular proteins from artichoke and asparagus agroindustrial waste using Rhodotorula mucilaginosa as a producer organism. Agricultural soil was collected from the Universidad Nacional de Trujillo (Peru), and R. mucilaginosa was isolated and identified using biochemical tests. Proteins and carotenoids were produced from artichokes and asparagus residues using the R. mucilaginosa yeast. Four substrate concentrations (10, 20, 30, and 40%) and a pH range (5–8.1) were used. They were incubated at 30 ◦C for 72 h. The results showed that protein and carotenoid yie...

In this study, we aimed to determine the in vitro activity of Leuconostoc mesenteroides var. mesenteroides isolated from sugar-industry effluents to produce a dextran bioflocculant from sucrose as a low-cost substrate. L. mesenteroides strains present in residual cane juice from a sugar factory were isolated and biochemically identified using Mayeux, Sandine, and Elliker agar (MSE) as a selective medium. The strain number 3 (LM03) was biochemically identified as L. mesenteroides var. mesenteroides, which was used for this study. The concentration of dextran was quantified by dry weight, the morphology and purity were evaluated using Fourier-transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDS). Flocculation was evaluated via turbidimetric assays in different pH ranges from sugar-industry effluents and doses of dextran. ...

Agroindustry waste has exponentially increased in recent years, generating economic losses and environmental problems. In addition, new ways to generate sustainable alternative electrical energy are currently being sought to satisfy energy demand. This investigation proposes using avocado waste as fuel for electricity generation in single-chamber MFCs. The avocado waste initially operated with an ambient temperature (22.4 ± 0.01 °C), DO of 2.54 ± 0.01 mg/L, TDS of 1358 ± 1 mg/L and COD of 1487.25 ± 0.01 mg/L. This research managed to generate its maximum voltage (0.861 ± 0.241 V) and current (3.781 ± 0.667 mA) on the fourteenth day, operating at an optimal pH of 7.386 ± 0.147, all with 126.032 ± 8.888 mS/cm of electrical conductivity in the substrate. An internal resistance of 67.683 ± 2.456 Ω was found on day 14 with a PD of 365.16 ± 9.88 mW/cm2 for a CD of 5.744 A/cm2. Micr...

Microbial fuel cells are a bioelectrochemical technology that uses different types of waste as fuel sources to generate sustainable and environmentally friendly electricity. Various MFCs have been developed, with the electrode used being a crucial problem due to its high manufacturing cost. This research shows that electrodes can be manufactured quickly and economically using activated carbon (100 g), sugar (80 g), ethanol (250 ml), and pine resin (200 g). For its demonstration, grape waste was used in a single-chamber MFC for 35 days. The manufactured electrode generated a Rint. of 18.471 ± 2.475 Ω, whose current density was 8.348 ±0.768 mW/cm2 at a current density of 5.166 A/cm2. The electrical potential shown was 0.889 ± 0.017 V and 4.571 ± 0.061 mA, with an ORPmax of 81.495 ± 1.874 mV, operating at a pH of 7.26 ±0.19. The micrographs made by scanning electron microscopy showed...

In Peru, from May 2021 to March 2022, hospital care was restricted due to the second and third wave of the COVID-19 pandemic. During this period a large number of cases of acute respiratory infections increased. With increasing resistance to antibacterials, the search for and identification of carbapenemase co-producing Enterobacteriaceae is of paramount therapeutic importance because it limits the use of available antibiotics. Objective: To determine the Enterobacterales species that present carbapenemase: KPC, NMD and OXA 48 in patients treated in a COVID hospital in Trujillo-Peru, during the COVID-19 pandemic. Methodology: In this descriptive, observational study, biological samples were collected from patients treated in general medicine, emergency, and intensive care units - ICU of Hospital IV "Víctor Lazarte Echegaray" for bacterial identification and sensitivity to carbapenems. r...

The enormous environmental problems that arise from organic waste have increased due to the significant population increase worldwide. Microbial fuel cells provide a novel solution for the use of waste as fuel for electricity generation. In this investigation, onion waste was used, and managedtogeneratemaximumpeaksof4.459±0.0608mAand0.991±0.02Vofcurrentandvoltage, respectively. The conductivity values increased rapidly to 179,987±2859 mS/cm, while the optimal pH in which the most significant current was generated was 6968 ± 0.286, and the ◦ Brix values decreased rapidly due to the degradation of organic matter. The microbial fuel cells showed a low internal resistance (154,389±5228 Ω), with a power density of 595.69±15.05 mW/cm2 at a current density of 6.02 A/cm2; these values are higher than those reported by other authors in the literature. The diffractogram spectra of the ...

“Research on the use of microbial biosensors for monitoring wastewater contaminants is a topic that covers few publications compared to their applicability in other fields, such as biomedical research. For this reason, a systematic analysis of the topic was carried out, for which research-type articles were reviewed during the period 2012 to September 2022. For this, different search platforms were used, including PubMed, ScienceDirect, Springer Link, and Scopus, and through the use of search equations a relevant bibliography was located. After that, the research articles were selected based on exclusion criteria. As a result, it was found that, of the 126 articles, only 16 articles were strictly related to the topic, since there was a duplication of articles among the different databases. It was possible to demonstrate the usefulness of microorganisms as components of biosensors to mo...

“Research on the use of microbial biosensors for monitoring wastewater contaminants is a topic that covers few publications compared to their applicability in other fields, such as biomedical research. For this reason, a systematic analysis of the topic was carried out, for which research-type articles were reviewed during the period 2012 to September 2022. For this, different search platforms were used, including PubMed, ScienceDirect, Springer Link, and Scopus, and through the use of search equations a relevant bibliography was located. After that, the research articles were selected based on exclusion criteria. As a result, it was found that, of the 126 articles, only 16 articles were strictly related to the topic, since there was a duplication of articles among the different databases. It was possible to demonstrate the usefulness of microorganisms as components of biosensors to mo...

Research on the use of microbial biosensors for monitoring wastewater contaminants is a topic that covers few publications compared to their applicability in other fields, such as biomedical research. For this reason, a systematic analysis of the topic was carried out, for which research-type articles were reviewed during the period 2012 to September 2022. For this, different search platforms were used, including PubMed, ScienceDirect, Springer Link, and Scopus, and through the use of search equations a relevant bibliography was located. After that, the research articles were selected based on exclusion criteria. As a result, it was found that, of the 126 articles, only 16 articles were strictly related to the topic, since there was a duplication of articles among the different databases. It was possible to demonstrate the usefulness of microorganisms as components of biosensors to monit...

The enormous environmental problems that arise from organic waste have increased due to the significant population increase worldwide. Microbial fuel cells provide a novel solution for the use of waste as fuel for electricity generation. In this investigation, onion waste was used, and managed to generate maximum peaks of 4.459 0.0608 mA and 0.991 0.02 V of current and voltage, respectively. The conductivity values increased rapidly to 179,987 2859 mS/cm, while the optimal pH in which the most significant current was generated was 6968 0.286, and the Brix values decreased rapidly due to the degradation of organic matter. The microbial fuel cells showed a low internal resistance (154,389 5228 W), with a power density of 595.69 15.05 mW/cm2 at a current density of 6.02 A/cm2; these values are higher than those reported by other authors in the literature. The diffractogram spectra of the on...

Research on the use of microbial biosensors for monitoring wastewater contaminants is a topic that covers few publications compared to their applicability in other fields, such as biomedical research. For this reason, a systematic analysis of the topic was carried out, for which research-type articles were reviewed during the period 2012 to September 2022. For this, different search platforms were used, including PubMed, ScienceDirect, Springer Link, and Scopus, and through the use of search equations a relevant bibliography was located. After that, the research articles were selected based on exclusion criteria. As a result, it was found that, of the 126 articles, only 16 articles were strictly related to the topic, since there was a duplication of articles among the different databases. It was possible to demonstrate the usefulness of microorganisms as components of biosensors to monit...

El puerto pesquero de Paita, desde hace varios años presenta contaminación por metales pesados a causa de la industria y del sector pesquero. El objetivo general fue determinar la eficacia de la fitorremediación con macrófitos de agua contaminada por plomo en el puerto pesquero de Paita – Piura. La metodología es aplicada, exploratoria, cuantitativa, experimental y transversal debido a las características de la información utilizada, la planta fitorremediadora utilizada fue Eichhornia crassipes (Jacinto de agua). Dentro de los resultados obtenidos se obtuvo: pH de 6,75 en la etapa inicial y 7,73 con la aplicación de macrófitos al 30 %; para el parámetro Conductividad eléctrica, presentó 16,27 uS/cm en la etapa inicial y 28,33 uS/cm post aplicación de macrófitos al 30 %; el parámetro de Oxígeno disuelto, el cual presentó 2,69 (mg/l) en la etapa inicial, luego de la apli...

The accelerated increase in energy consumption by human activity has generated an increase in the search for new energies that do not pollute the environment, due to this, microbial fuel cells are shown as a promising technology. The objective of this research was to observe the influence on the generation of bioelectricity of sucrose, with different percentages (0%, 5%, 10% and 20%), in papaya waste using microbial fuel cells (MFCs). It was possible to generate voltage and current peaks of 0.955 V and 5.079 mA for the cell with 20% sucrose, which operated at an optimal pH of 4.98 on day fifteen. In the same way, the internal resistance values of all the cells were influenced by the increase in sucrose, showing that the cell without sucrose was 0.1952 ± 0.00214 KΩ and with 20% it was 0.044306 ± 0.0014 KΩ. The maximum power density was 583.09 mW/cm2 at a current density of 407.13 A/cm...

The environmental problems caused by the excessive use of fossil fuels for electricity generation have led to the development of new technologies. Microbial fuel cells constitute a technology that uses organic sources for electricity generation. This research gives a novel means of using Golden Berry waste as fuel for electricity generation through microbial fuel cells made at low cost, achieving current and voltage peaks of 4.945 ± 0.150 mA and 1.03 ± 0.02 V, respectively. Conductivity values increased up to 148 ± 1 mS/cm and pH increased up to 8.04 ± 0.12 on the last day. The internal resistance of cells was 194.04 ± 0.0471 Ω, while power density was 62.5 ± 2 mW/cm2 at a current density of 0.049 A/cm2. Transmittance peaks of the Fourier-transform infrared (FTIR) spectrum showed a decrease when comparing the initial and final spectra, while the bacterium Stenotrophomonas maltoph...

“The increase in the population and its need to produce food has caused the level of contamination by organic waste to increase exponentially in recent years. Innovative methods have been proposed for the use of this waste and thus to mitigate its impact. One of these is to use it as fuel in microbial fuel cells to generate electricity. This research aims to generate bioelectricity using coriander waste in microbial fuel cells. The maximum voltage and current observed were 0.882 ± 0.154 V and 2.287 ± 0.072 mA on the seventh and tenth day, respectively, these values were obtained working at an optimum operating pH of 3.9 ± 0.16 and with an electrical conductivity of 160.42 ± 4.54 mS/cm. The internal resistance observed in the cells was 75.581 ± 5.892 Ω, with a power density of 304.325 ± 16.51 mW/cm2 at 5.06 A/cm2 current density. While the intensity of the final FTIR (Fourier tra...

The environmental problems caused by the excessive use of fossil fuels for electricity generation have led to the development of new technologies. Microbial fuel cells constitute a technology that uses organic sources for electricity generation. This research gives a novel means of using Golden Berry waste as fuel for electricity generation through microbial fuel cells made at low cost, achieving current and voltage peaks of 4.945 ± 0.150 mA and 1.03 ± 0.02 V, respectively. Conductivity values increased up to 148 ± 1 mS/cm and pH increased up to 8.04 ± 0.12 on the last day. The internal resistance of cells was 194.04 ± 0.0471 Ω, while power density was 62.5 ± 2 mW/cm2 at a current density of 0.049 A/cm2. Transmittance peaks of the Fourier-transform infrared (FTIR) spectrum showed a decrease when comparing the initial and final spectra, while the bacterium Stenotrophomonas maltoph...

“A large amount of fruit waste is being a great environmental and social problem due to a lack of adequate storage. Among the most abundant waste is papaya, due to its high consumption in various varieties. These wastes can generate bioelectricity through organic waste, being an important parameter the pH. In this research, lowcost laboratory-scale microbial fuel cells were fabricated, using papaya waste as fuel at different pH (4, 5.73, 7, and 9) to obtain the optimum operating pH. It was possible to observe the maximum values of electric current and voltage of 17.97 mA and 1.02 V on days 16 and 14, in the cell with pH 7; while the cell with pH was the one that showed the lowest values. The electrical conductivity values increased from the first day, observing a maximum peak of 172.50 mS/cm for the cell with pH 7. However, the internal resistance values were low, the maximum value bei...