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1
tesis de grado
The objective of this research was to isolate and identify heat-resistant bacteria from canned canned Bactris gasipaes "palmito" from Caynarachi S.A. From a batch of 265 units, 8 preserves with signs of bulging were selected, according to the Peruvian Technical Standard ISO 2859-1. Selected canned samples were incubated for 10 days at 55 ° C. For enrichment, 1 g of crushed heart of each sample was placed in duplicate in 9 ml of BHI broth at a temperature of 55 ° C for 48 h, where microorganisms were obtained in aerobic and anaerobic conditions due to the presence of turbidity. For the isolation, serial dilutions of 10-1 to 10-4 were made and 0.1 ml of the last two dilutions in BHI and PCA agar were seeded, which were incubated to the same initial conditions (aerobic and anaerobic). The macroscopic and microscopic characteristics of the isolated colonies were observed, by means of Gram ...
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artículo
Microorganisms’ degradation of agro-industrial waste produces bad odors and greenhouse gases that contribute to global warming. Consequently, eco-friendly, sustainable biotechnological alternatives to this waste are sought to provide additional value, which is why this study’s objective was to develop a method of producing unicellular proteins from artichoke and asparagus agroindustrial waste using Rhodotorula mucilaginosa as a producer organism. Agricultural soil was collected from the Universidad Nacional de Trujillo (Peru), and R. mucilaginosa was isolated and identified using biochemical tests. Proteins and carotenoids were produced from artichokes and asparagus residues using the R. mucilaginosa yeast. Four substrate concentrations (10, 20, 30, and 40%) and a pH range (5–8.1) were used. They were incubated at 30 ◦C for 72 h. The results showed that protein and carotenoid yie...
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artículo
Phenolic compounds have gained international interest due to their carcinogenic, toxic, and bioaccumulative properties, causing adverse effects in both animals and humans. As a result, there is a growing interest in finding alternative and eco-friendly treatment routes for phenol by exploring new microbial cultures with potential adaptation and biodegradation capabilities. In this study, the phenol removal efficiency of Yarrowia lipolytica YQ22 under laboratory conditions was determined. The YQ22 strain was obtained from diesel samples from a fuel station in Trujillo, Peru, isolated through serial dilutions on Sabouraud agar, and identified through its morphological characteristics using microscopy and molecular analysis by polymerase chain reaction of the ribosomal DNA internal transcribed spacer (ITS) and 5.8S regions. In the treatment, the effect of pH (5, 6 and 7) and temperature (25...