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informe técnico
Publicado 2021
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Peru is a place with abundant biological resources that should be employed for the benefit of society in general. However, to date, the use of Peruvian plant genetic resources was not fully exploited for the development of improved crops. This work was mostly conducted by the international private sector. The Climate Change Laboratory at Instituto Nacional de Innovación Agraria, and other laboratories at Universidad Nacional José Faustino Sánchez Carrión and Universidad Nacional Agraria La Molina together with other research programs of other institutions seek to promote the massive and sustainable use of plant genetic resources maintained in germplasm banks. It is planned to make use of modern molecular and morphological techniques. Moreover, infrastructure and human resources are being improved. As a result, we will be able to maintain the growth of the agricultural activity in Per...
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artículo
Publicado 2022
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Loche is an ancient landrace of squash from Northern Peru, notable for its vegetative re-production and lack of seeds in fruits. To date, very little is known about its genetics. Here, we used 21 simple sequence repeats to assess the genetic diversity and population structure of a collection of 100 samples of loche from three localities in Peru, and 10 samples of related species, C. pepo and C. maxima (110 accessions in total). A total 85 bands were manually scored, obtaining an average of 4.05 alleles per locus. UPGMA clustering method and principal coordinate analysis showed a clear identification between the three species of Cucurbita. Population structure analysis clustered the 110 accessions into five populations: (i) three of loche, (ii) one of C. pepo, and (iii) one of C. maxima. Genetic diversity estimation was conducted considering only the three groups (populations) of loche id...
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artículo
Publicado 2023
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El Pisco stands as Peru's distilled spirit, crafted from recently fermented musts derived from native grape varieties known as "pisqueras". Yeasts serve as decisive microorganisms in the fermentation process, and the utilization of selected native strains confers competitive advantages for product typicity, standardization, and microbiological control within the production process. The aim was to identify and select native yeast strains for Quebranta grape Pisco production, isolated from Pisco production processes in the Ica Valley. Microbiological and molecular techniques were employed, utilizing ITS1-5.8S-ITS2 PCR-RFLP analysis for taxonomic identification. The assessment of yeast strains for Pisco production involved the physicochemical and organoleptic analysis of the distilled product obtained using the selected strains. Three isolates were evaluated for Pisco production, identified...
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artículo
Publicado 2021
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Se utilizaron tres coeficientes de similitud: Simple Matching (SM), Jaccard (J) y Dice (D) para analizar la variabilidad genética mediante el uso de marcadores moleculares AFLP en 48 accesiones de Lupinus Mutabilis Sweet, 26 provenientes del Departamento de Cusco y 22 de departamentos y países distintos (Ecuador y Bolivia). A partir de matrices de similitud, se generaron dendrogramas con cada coeficiente, mediante el método UPGMA a través del software NTSYSpc versión 2.01, analizándose y comparándose entre sí, obteniendo un Coeficiente de correlación cofenética (r): SM=0,68674, J=0,79116 y D=0,79332; un Índice de consenso (Clc): SM–J=0,52, SM–D=0,52 y J–D=0,93; y niveles de similitud: SM=0,68, J=0,39 y D=0,56. Determinándose el coeficiente de Simple Matching como el más idóneo para análisis de variabilidad genética con marcadores dominantes como AFLP en L. mutabilis...
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artículo
Publicado 2020
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Tomato susceptibility/resistance to stem canker disease caused by Alternaria alternata f. sp. lycopersici and its pathogenic factor AAL-toxin is determined by the presence of the Asc1 gene. Several cultivars of commercial tomato (Solanum lycopersicum var. lycopersicum, SLL) are reported to have a mutation in Asc1, resulting in their susceptibility to AAL-toxin. We evaluated 119 ancestral tomato accessions including S. pimpinellifolium (SP), S. lycopersicum var. cerasiforme (SLC) and S. lycopersicum var. lycopersicum “jitomate criollo” (SLJ) for AAL-toxin susceptibility. Three accessions, SP PER018805, SLC PER018894, and SLJ M5-3, were susceptible to AAL-toxin. SLC PER018894 and SLJ M5-3 had a two-nucleotide deletion (nt 854_855del) in Asc1 identical to that found in SLL cv. Aichi-first. Another mutation (nt 931_932insT) that may confer AAL-toxin susceptibility was identified in SP PE...
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artículo
Publicado 2021
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Se utilizaron tres coeficientes de similitud: Simple Matching (SM), Jaccard (J) y Dice (D) para analizar la variabilidad genética mediante el uso de marcadores moleculares AFLP en 48 accesiones de Lupinus Mutabilis Sweet, 26 provenientes del Departamento de Cusco y 22 de departamentos y países distintos (Ecuador y Bolivia). A partir de matrices de similitud, se generaron dendrogramas con cada coeficiente, mediante el método UPGMA a través del software NTSYSpc versión 2.01, analizándose y comparándose entre sí, obteniendo un Coeficiente de correlación cofenética (r): SM=0,68674, J=0,79116 y D=0,79332; un Índice de consenso (Clc): SM–J=0,52, SM–D=0,52 y J–D=0,93; y niveles de similitud: SM=0,68, J=0,39 y D=0,56. Determinándose el coeficiente de Simple Matching como el más idóneo para análisis de variabilidad genética con marcadores dominantes como AFLP en L. mutabilis...
