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transport protein » transfer protein (Expander búsqueda), transport routes (Expander búsqueda), transport properties (Expander búsqueda)
peru transport » air transport (Expander búsqueda), new transport (Expander búsqueda), por transporte (Expander búsqueda)
1
artículo
Publicado 2019
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The aim of this study was to characterize the PepT1 of pirarucu Arapaima gigas through genomic and proteomic tools. A transcriptomic and proteomic analysis was made from sections of the intestine, spleen, liver and kidney of fingerlings. The transcriptomic analysis allowed obtaining two consensus sequences of 357 and 459 bp. Both fragments showed a high degree of homology (78 and 80%), mainly with nucleotide sequences coding for the PepT1 of Scleropages formosus of the same Order as A. gigas. The proteomic analysis by double mass spectrometry MALDI TOF/TOF allowed to identify 15 peptide sequences of PepT1 in pirarucu. In conclusion, PepT1 was partially characterized in the intestine of pirarucu, which could be used for further studies on the evaluation of its expression.
2
artículo
Publicado 2019
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The aim of this study was to characterize the PepT1 of pirarucu Arapaima gigas through genomic and proteomic tools. A transcriptomic and proteomic analysis was made from sections of the intestine, spleen, liver and kidney of fingerlings. The transcriptomic analysis allowed obtaining two consensus sequences of 357 and 459 bp. Both fragments showed a high degree of homology (78 and 80%), mainly with nucleotide sequences coding for the PepT1 of Scleropages formosus of the same Order as A. gigas. The proteomic analysis by double mass spectrometry MALDI TOF/TOF allowed to identify 15 peptide sequences of PepT1 in pirarucu. In conclusion, PepT1 was partially characterized in the intestine of pirarucu, which could be used for further studies on the evaluation of its expression. © 2019 Universidad Nacional Mayor de San Marcos. All rights reserved.
3
artículo
Publicado 2025
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The chame Dormitator latifrons is a symbolic species associated with rural aquaculture in coastal regions of the South American Pacific. Its adaptation to stay alive out of water (anhydrobiosis) for days is beneficial for its storage and transport at room temperature. The aim of the study was to determine the tolerance of chame to anhydrous conditions. Sixty chames were distributed in containers and kept in two climate-controlled environments at 16 and 24 °C for 96 h. Survival, malondialdehyde levels, total proteins and pH in the muscles and lactate dehydrogenase (LDH) activity in the gills were monitored. Additionally, the behaviour of the fish was monitored, as well as the total number of microorganisms with a sensory evaluation. The critical period of survival occurred after 48 h, where the pH decreased, and the MDA levels increased. The number of microorganisms increased in both en...
4
artículo
Publicado 2022
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The aim of this study was to evaluate the sedative effect of three products: valerian extract (Valeriana officinalis), benzocaine and tricaine methanesulfonate (MS-222) on stress levels produced by a simulated transport model in tilapia fingerlings. In a first part, the sedative dose of the products was determined based on the clinical characteristics that indicate depression of the central nervous system. In the second part, sedation and subsequent simulated transport for 3 hours were performed. The fish were euthanized, and blood samples were taken from the tail vein to determine cortisol (ELISA) and glucose (portable glucometer) levels, haematological parameters and mortality. The optimal doses of sedation were 200, 35 and 75 mg/l, respectively, for valerian extract, benzocaine and MS-222. After 3 h of simulated transport, cortisol and glucose values were similar or higher compared to...
5
Publicado 2019
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El objetivo de este trabajo de investigación fue las características estructurales de la célula en general son la individualidad, la sistematicidad y la tridimensionalidad. Las células contienen citosol y material genético y poseen enzimas y diversas proteínas. La membrana celular es una estructura formada por una bicapa lipídica que envuelve y delimita a la célula en su conjunto. El núcleo celular es un orgánulo membranoso en forma de esfera, localizado por lo general en la zona central de las células eucariotas. Contiene la mayor parte de la carga genética celular bajo la forma de moléculas muy largas de ADN. Estructuralmente, el núcleo celular consta de envoltura nuclear, poros nucleares, nucléolo, lámina nuclear, cromatina y cromosoma, nucleoplasma, otros cuerpos subnucleares (cuerpos de Cajal, PIKA, cuerpos PML, paraspeckles, speckles, cuerpos de escisión, cuerpos D...
6
artículo
Publicado 2019
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El objetivo del estudio fue caracterizar el PepT1 del paiche Arapaima gigas mediante herramientas genómicas y proteómicas. Se realizó un análisis transcriptómico y proteómico a partir de secciones del intestino, bazo, hígado y riñón de alevines. El análisis transcriptómico permitió obtener dos secuencias consenso de 357 y 459 pb. Ambos fragmentos presentaron un alto grado de homología (78 y 80%), principalmente con secuencias de nucleótidos codificantes del PepT1 de Scleropages formosus del mismo Orden que el paiche. El análisis proteómico por espectrometría de doble masa MALDI TOF/TOF permitió identificar quince secuencias peptídicas del PepT1 en paiche. Como conclusión se logró caracterizar parcialmente el PepT1 en el intestino del paiche, las cuales podrán ser usadas para posteriores estudios sobre la evaluación de su expresión.
7
tesis de grado
Publicado 2025
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En este estudio se diseñó e implementó una planta piloto automatizada para la producción de leche de Tarwi, con capacidad para procesar 10 kilogramos de grano desamargado por lote. El propósito principal fue modernizar y estandarizar las etapas de molienda, cocción y pasteurización, incorporando sistemas de instrumentación y control que aseguren la calidad y la inocuidad del producto final. El sistema integra un Controlador Lógico Programable (PLC Siemens S7-1200), sensores de temperatura PT100, resistencias tipo banda para el calentamiento y un intercambiador de calor de placas para el enfriamiento. La regulación térmica se realizó mediante un algoritmo PID en lazo cerrado, lo que permitió mantener una curva estable y alcanzar una pasteurización efectiva a 75 °C durante 15 minutos, reduciendo la presencia de patógenos y conservando el contenido proteico del producto. El ...
8
tesis de maestría
Publicado 2019
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Universidad Nacional Agraria La Molina. Escuela de Posgrado. Maestría en Nutrición
9
tesis de maestría
Universidad Nacional Agraria La Molina. Escuela de Posgrado. Maestría en Mejoramiento Genético de Plantas
10
artículo
The aim of this study was to determine the postnatal development of Islets of Langerhans (IL) in alpacas of 0 to 45 days of age. The animals were divided in nine age groups with range of five days. The mumber of IL varied from 3.03 to 4.46 in 10x fields (with an ocular of 10x), the IL diameter varied from 67.89 to 72.55 μm, the number of β cells in the IL from 23.79 to 47.08 and other cells from 18.15 to 39.93. Moreover, the insulin receptor and the transport protein GLUT-2 were observed by immunohistochemistry on the first day of life. It is concluded that the number and size of IL increased with age (p<0.05) while β cells do not show the same trend.
11
artículo
The aim of this study was to determine the postnatal development of Islets of Langerhans (IL) in alpacas of 0 to 45 days of age. The animals were divided in nine age groups with range of five days. The mumber of IL varied from 3.03 to 4.46 in 10x fields (with an ocular of 10x), the IL diameter varied from 67.89 to 72.55 μm, the number of β cells in the IL from 23.79 to 47.08 and other cells from 18.15 to 39.93. Moreover, the insulin receptor and the transport protein GLUT-2 were observed by immunohistochemistry on the first day of life. It is concluded that the number and size of IL increased with age (p<0.05) while β cells do not show the same trend.
12
artículo
Publicado 2019
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Se analizaron las características de una cepa de Bacillus amyloliquefaciens aislada del intestino de Arapaima gigas, seleccionada por su capacidad para generar inhibición de múltiples bacterias patógenas de peces, mediante el uso de herramientas moleculares como PCR y espectrometría de masas - MALDI TOF/TOF. Los resultados mostraron a través de PCR que esta cepa cuenta con genes claves para la generación de péptidos antimicrobianos como bmyB (bacillomycin L synthetase B), fenD (fengycin sintetasa), srfAA (subunidad 1 surfactin sintetasa), bacA (proteína de biosíntesis de bacilysin) e iturin (iturin A). Además, mediante el análisis por espectrometría de masas se detectaron bacteriocinas (plipastatin, gramicidin, fengycin, surfactin), proteínas de fijación al intestino (like-enolase), proteinas transportadoras de péptidos antimicrobianos (ABC-transporters) y proteínas de e...
13
artículo
Publicado 2019
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The characteristics of a strain of Bacillus amyloliquefaciens isolated from the intestine of Arapaima gigas, selected for its ability to generate inhibition of multiple pathogenic bacteria of fishes were analyzed by using molecular tools such as PCR and mass spectrometry - MALDI TOF / TOFM. The results showed through PCR that this strain has key genes for the generation of antimicrobial peptides such as bmyB (bacillomycin L synthetase B), fenD (fengycin synthetase), srfAA (subunit 1 surfactin synthetase), bacA (bacilysin biosynthesis protein) and iturin (iturin A). In addition, by mass spectrometry analysis were detected bacteriocins (plipastatin, gramicidin, fengycin, surfactin), intestine binding proteins (like-enolase), antimicrobial peptide transport proteins (ABC-transporters) and immune system stimulation proteins like flagelin. Collagenase, chitinases and xylose-isomerases protein...
14
artículo
Publicado 2013
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Iron is essential for health but in excess may be harmful. The living organism has ability to store and re-use the iron body and as such its requirement is small (1-2 mg absorbed/day). Hepcidin, a hormone produced in the liver, has an important role in iron homeostasis by blocking the iron transport protein and inhibiting iron absorption. Maternal anemia due to iron deficiency is a public health problem when it is moderate (7-<9 g/dL) and severe (Hb<7 g/dL) since it increases the risk of maternal and neonatal morbi-mortality. Similarly, high levels of hemoglobin during pregnancy affect the mother and newborn. In Peru, there is a National Program to supplement with iron to all pregnant women anemic or not. Scientific evidence shows that hemoglobin values between 9-10.5 g/dL are optimal for best fetal growth and that treatment with iron supplementation to non anemic women increases levels ...
15
artículo
Publicado 2013
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Iron is essential for health but in excess may be harmful. The living organism has ability to store and re-use the iron body and as such its requirement is small (1-2 mg absorbed/day). Hepcidin, a hormone produced in the liver, has an important role in iron homeostasis by blocking the iron transport protein and inhibiting iron absorption. Maternal anemia due to iron deficiency is a public health problem when it is moderate (7-<9 g/dL) and severe (Hb<7 g/dL) since it increases the risk of maternal and neonatal morbi-mortality. Similarly, high levels of hemoglobin during pregnancy affect the mother and newborn. In Peru, there is a National Program to supplement with iron to all pregnant women anemic or not. Scientific evidence shows that hemoglobin values between 9-10.5 g/dL are optimal for best fetal growth and that treatment with iron supplementation to non anemic women increases l...
16
artículo
Publicado 2021
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Potato virus X (PVX) occurs worldwide and causes an important potato disease. Complete PVX genomes were obtained from 326 new isolates from Peru, which is within the potato crop′s main domestication center, 10 from historical PVX isolates from the Andes (Bolivia, Peru) or Europe (UK), and three from Africa (Burundi). Concatenated open reading frames (ORFs) from these genomes plus 49 published genomic sequences were analyzed. Only 18 of them were recombinants, 17 of them Peruvian. A phylogeny of the non-recombinant sequences found two major (I, II) and five minor (I-1, I-2, II-1, II-2, II-3) phylogroups, which included 12 statistically supported clusters. Analysis of 488 coat protein (CP) gene sequences, including 128 published previously, gave a completely congruent phylogeny. Among the minor phylogroups, I-2 and II-3 only contained Andean isolates, I-1 and II-2 were of both Andean and...
17
informe técnico
Publicado 2025
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About IMMUNOPERU 2025: This IUIS-ALACI-PSI course has brought together a diverse group of students, immunologists and experts to share their distinct perspectives, skills and expertise. The focus was on a variety of endemic and emerging diseases related to environmental and climate change. The goal was to catalyze the development of novel concepts, methods and approaches in addressing these emerging challenges. MAIN TOPICS: The course has addressed the Impact of environmental exposures on immunity in infectious disease, chronic inflammation and cancer with particular attention to: Changes in microbial communities and their metabolites that drive inflammatory disease (eg. effects of antibiotics, dietary and other factors) Environmental cues and pollutants linked to the development of hematologic malignancies. The impact of climate change on endemic infectious diseases (eg. Tuberculosis, D...
18
tesis de grado
Publicado 2007
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Son ampliamente conocidas las ventajas de los productos vegetales y frutas secas, ya que, al reducir su contenido de humedad, disminuye notablemente la actividad microbiológica y enzimática, además, disminuye los costos de almacenamiento y transporte. El presente trabajo, tuvo por objetivo obtener rodajas de piña (Ananas comosus) seca, variedad Cayena lisa, utilizando procesos combinados de deshidratación osmótica y secado; fueron utilizados como osmodeshidratantes sacarosa y etanol. La composición proximal de la piña fresca con humedad, proteínas, grasa, cenizas, fibra y carbohidratos totales de: 87.64 %, 0.14 %, 0.12%, 0.30 %, 0.39 % y 11.38 % respectivamente; el contenido de ácido ascórbico fue de 26.85 mg/100g- previamente a la deshidratación osmótica se verifico que las rodajas de piña de 1 cm de espesor, estén en condiciones óptimas de frescura, se les retiro el cor...
19
artículo
Publicado 2023
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The empirical use of alpaca rennet in the production of artisanal cheeses with cow’s milk is widespread in the high Andean communities of Perú and Bolivia, and has not been scientifically reported. The objective of the present study was to characterize alpaca rennet and evaluate its coagulating activity. Ready-to-use alpaca curds were collected directly from dairies in four neighborhoods of Viscapalca (Huancavelica-Perú, 4003 masl), which were transported under refrigeration and kept frozen at -20°C. The Crude Enzymatic Extract (CEE) was obtained by centrifugation at 560 x g for 10 min at 4°C, from which proteins were determined by Bradford, SDS-PAGE electrophoretic profile, coagulation time and proteolytic activity. The traditional management of alpaca rennet is also described. CEE was found to contain 29.80 to 52.91 µg/ml of protein. The SDS-PAGE electrophoretic profile revealed...
20
artículo
Publicado 2023
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The empirical use of alpaca rennet in the production of artisanal cheeses with cow’s milk is widespread in the high Andean communities of Perú and Bolivia, and has not been scientifically reported. The objective of the present study was to characterize alpaca rennet and evaluate its coagulating activity. Ready-to-use alpaca curds were collected directly from dairies in four neighborhoods of Viscapalca (Huancavelica-Perú, 4003 masl), which were transported under refrigeration and kept frozen at -20°C. The Crude Enzymatic Extract (CEE) was obtained by centrifugation at 560 x g for 10 min at 4°C, from which proteins were determined by Bradford, SDS-PAGE electrophoretic profile, coagulation time and proteolytic activity. The traditional management of alpaca rennet is also described. CEE was found to contain 29.80 to 52.91 µg/ml of protein. The SDS-PAGE electrophoretic profile revealed...
