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protease producing » protease production (Expander búsqueda), proteases production (Expander búsqueda), proteases produced (Expander búsqueda)
para protease » para protegerse (Expander búsqueda), para proteger (Expander búsqueda), para protesis (Expander búsqueda)
1
artículo
Publicado 2017
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Microbial proteases are the most important group in the enzymes world market, because have several industrial applications. Moreover, they have some advantages respect to proteases produced by other sources, such as to have more stability and to be excreted to fermentation medium. The genus Pseudomonas has been studied as a protease producer of enzymes with industrial potential. Nevertheless, the production is affected by factors such as medium composition and culture conditions, and their optimization lead to reduce costs and increase yield. In this context, medium components that influenced significantly in production of Pseudomonas sp. M211 extracellular proteases in submerged fermentation were determined. In this work, one-factor-at-a-time method was used to assess the effect of different carbon, nitrogen and ions sources in proteases production. The highest level of extracellular pr...
2
artículo
Publicado 2025
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This study aimed to investigate protease and hydrolysate production by Pseudomonas sp. SU24, isolated from soils in Bongará province (Amazonas Department, Peru), using poultry waste as culture substrate. Enzyme production was evaluated by assessing incubation time, poultry waste concentration, and pH through a onefactor approach followed by central composite design. Production under optimized conditions was compared with skim milk and tryptic soy broth (TSB) media, while the proteolytic profile was analyzed by zymography. Biological activity prediction was performed through in silico hydrolysis of chicken feather keratin using the BIOPEP-UWM database, complemented with antioxidant activity assays (ABTS). Maximum protease production (2972.77 ± 23.89 U/mL) was achieved in the optimized medium containing 20 g/L feathers at pH 9.0 and 37°C after 96 h incubation, significantly outperformin...
3
artículo
Publicado 2023
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Objective: The current study’s objective is to characterize a new throm-bin-like enzyme called TLBro that was obtained from Bothrops roedingeris snake from a biochemical and hemostatic perspective. Methodology: One chromatographic step was used to purify it, producing the serine protease TLBro. Molecular mass was estimated by SDS-PAGE to be between reduced and unreduced by 35 kDa. Tryptic peptide sequencing using Swiss Prot provided the complete amino acid sequence. Expasy.org by conducting a search that is limited to Crotalinae snake serine proteases and displaying a high degree of amino acid sequence. Results: Ser (182) is inhibited by phenylmethylsulfonyl fluoride (PMSF), and TLBro demonstrated the presence of Asp (88) residues. It also deduced the positions of His (43) and Ser (182) in the set of three coordinated amino acids in serine proteases. It was discovered that this substra...
4
artículo
Publicado 2021
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Bacteria and microbial enzymes are biocatalysts and can be used as an alternative to industrial chemical processes. The present study focused on isolating and identifying bacterial strains from shrimp waste, that produce amylases, lipases, proteases and chitinases with potential use on shrimp waste treatment. Thirty-two bacterial strains were isolated, phenotypically characterized, and identified by the API system and the molecular analysis of the 16S rDNA. It was found that 28.13% of the isolated bacterial strains had amylolytic capacity, 87.50% lipolytic, 96.88% proteolytic and 28.13% chitinolytic capacity on agar plates with specific substrates. The genera Bacillus, Burkholderia, Ochrobactrum, Vibrio, Pseudomonas and Shewanella were identified. Bacteria with enzymatic capacities isolated in the present study, could be used to obtain by-products from shrimp waste as well as other indus...
5
artículo
Publicado 2021
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Bacteria and microbial enzymes are biocatalysts and can be used as an alternative to industrial chemical processes. The present study focused on isolating and identifying bacterial strains from shrimp waste, that produce amylases, lipases, proteases and chitinases with potential use on shrimp waste treatment. Thirty-two bacterial strains were isolated, phenotypically characterized, and identified by the API system and the molecular analysis of the 16S rDNA. It was found that 28.13% of the isolated bacterial strains had amylolytic capacity, 87.50% lipolytic, 96.88% proteolytic and 28.13% chitinolytic capacity on agar plates with specific substrates. The genera Bacillus, Burkholderia, Ochrobactrum, Vibrio, Pseudomonas and Shewanella were identified. Bacteria with enzymatic capacities isolated in the present study, could be used to obtain by-products from shrimp waste as well as other indus...
6
tesis de grado
Universidad Nacional Agraria La Molina. Facultad de Industrias Alimentarias. Departamento Académico de Tecnología de Alimentos y Productos Agropecuarios
7
tesis de grado
Publicado 2005
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Del veneno del escorpión Brachistosternus ehrenbergii, se ha aislado una toxina específica para ratones. La toxina denominada Be1 fue purificada mediante cromatografía de intercambio catiónico, en CM-Sephadex C-25 (16 x 1,1 cm) con buffer acetato de amonio 0,05 M a pH 7, y se caracteriza por ser una proteína básica que constituye el 8,1 % de la proteína total del veneno. La pureza de la toxina fue evaluada por electroforesis en condiciones nativas, de acuerdo al método de Reisfeld, y en condiciones denaturantes por el método de Schägger y von Jagow, determinándose que la toxina es de una sola cadena polipeptídica de 6,3 kDa. La toxina al ser inoculada en ratones albinos adultos; vía intraperitoneal (62 µg) produce la aparición de algunos signos locales como hipersecreción salival seguido por cuadros de afección respiratoria, arrastre de las patas posteriores, hasta causa...
8
tesis de grado
Publicado 2024
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La pandemia producida a finales del 2019 (COVID-19) causada por el virus SARS-CoV- 2 condujo a la búsqueda de potenciales inhibidores del SARS-CoV-2 para el tratamiento de la enfermedad. El objetivo principal de la presente tesis es el emplear técnicas in silico además de principios de Química Medicinal para diseñar complejos de Ga candidatos a inhibidores de la proteasa Mpro (blanco), proteína esencial en la replicación del virus. A partir de un inhibidor conocido (N3) de la proteasa Mpro (PDB ID 6LU7) se realizaron modificaciones estructurales empleando una lista de fragmentos bioisósteros, obteniéndose estructuras modificadas de N3 (ligandos), los que fueron prepararon en Google Colaboratory. Por otro lado, se preparó Mpro (ID 6W63) utilizando Chimera 1.15 para seguidamente ejecutar el virtual screening en una supercomputadora (MANATÍ). Se consiguieron acoplamientos con may...
9
tesis de grado
Publicado 2017
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Alkaline proteases are a physiologically and commercially important group, used primarily as detergent additives and food processing. The objective of this research was to isolate and select native Bacillus sp. Protease producers from compost of the Experimental Station of Applied Biochemistry, National University of Trujillo, Perú. For this purpose, compost was collected as a sample, enriched with a 1% casein solution, and a thermal treatment (80 °C for 15 minutes). To isolate the native Bacillus, serial dilutions were made, and 0.1 mL per surface were plated on Casein Agar plates and incubated at 37 ° C for 24 to 48 hours. The selected bacterial cultures were placed in stirred system bioreactors containing Casein Broth at room temperature (23 ° C - 25 ° C), and every 24 hours (0, 24, 48, 72, 96, 120 and 144 h) was determined the production of proteases by extracting 8 mL of sample...
10
tesis de maestría
Publicado 2024
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La proteína FhT es una cisteína proteasa con actividad fibrinolítica in vitro. La purificación de esta enzima de su organismo de origen resulta en cantidades limitadas y una baja pureza, además de ser un proceso costoso y laborioso. En esta investigación, empleamos tecnología de ADN recombinante para superar estas limitaciones. El objetivo fue producir la proteína FhT en su forma recombinante y evaluar su actividad fibrinolítica in vitro. Se clonó el gen de la forma zimógena de FhT (rProFhT) en el vector pPICZαC y se recombinó con el genoma de Pichia pastoris X-33. Se expresó rProFhT de 37 kDa y se purificó con un rendimiento de 20 mg/L. La activación de rProFhT a su forma enzimáticamente activa (rFhT) de 25 kDa se logró mediante la incubación con 10 mM de DTT y 1 mM de EDTA a pH 5 durante 8 horas. La enzima alcanzó su actividad óptima a un pH de 5 y mantenía activi...
11
artículo
Publicado 2020
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The aim of this work was to evaluate the effect of Saccharomyces cerevisiae supplementation plus an enzyme complex (amylases, lipases and proteases) on daily body weight gain and blood urea level in Holstein Friesian calves from 5 to 61 days of age. The daily weight gain was 514 versus 462 g/day of the control group. The urea level was significantly higher in the experimental group (30.11 mg/dl) than in the control group (22.98 mg/dl) (p<0.01). It is concluded that dietary supplementation of S. cerevisiae and enzymes favors protein metabolism in calves, although it is not sufficient to produce an improvement in daily weight gain.
12
artículo
Publicado 2020
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The aim of this work was to evaluate the effect of Saccharomyces cerevisiae supplementation plus an enzyme complex (amylases, lipases and proteases) on daily body weight gain and blood urea level in Holstein Friesian calves from 5 to 61 days of age. The daily weight gain was 514 versus 462 g/day of the control group. The urea level was significantly higher in the experimental group (30.11 mg/dl) than in the control group (22.98 mg/dl) (p<0.01). It is concluded that dietary supplementation of S. cerevisiae and enzymes favors protein metabolism in calves, although it is not sufficient to produce an improvement in daily weight gain.
13
artículo
Publicado 2025
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The banana (Musa AAA) is affected by Fusarium oxysporum f. sp. cubense, which causes discoloration in the xylem duct, leading to terminal wilting. The use of plant growth promoting rhizobacteria (PGPR) as a biological control produces different antagonistic compounds and inhibits the growth of various phytopathogens. The objective of the study was based on the molecular identification of rhizobacteria that produce phytohormones with biocontrol activity against Foc-R1. The presence of the 225 bp ChiA gene was observed in PGPR. Phylogenetic analysis of 16S rRNA by sequencing and ERIC-PCR showed genetic variability with the formation of four subgroups. Molecular identification by sequencing the 16S rRNA gene defined the genera as Klebsiella, Enterobacter, and Pseudomonas. There is variation in the biosynthesis of the phytohormones AIA, AG, and AS in strains MH-18, W-417, and FZ 9-7 at 72 h....
14
artículo
Publicado 2010
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In order to isolate halotolerant bacteria with lipolytic activity from the Pilluana Salterns in San Martin, saline soil samples were collected and cultured in 5% salt water agar containing 0,5% yeast extract. It was pre-selected 55 bacteria with different morphological characteristics, these bacteria were grown simultaneously in 5% salt water agar containing 1% tributyrin, Tween 80 and olive oil. It was selected 14 bacteria based on the ability to hydrolyze specific substrates and shown high salt tolerance rate between 0 to 25%. These bacteria grew optimally at pH 7 and 37 °C, six were Gram positive and Gram negative eight, 12 bacteria hydrolyzed tributyrin, Tween 80 and olive oil, six produced amylases and proteases. The 14 halotolerant bacteria with lipolytic activity isolated from the Pilluana salterns are promising lipase production sources and oily waste bio-treatment.
15
artículo
Publicado 2010
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In order to isolate halotolerant bacteria with lipolytic activity from the Pilluana Salterns in San Martin, saline soil samples were collected and cultured in 5% salt water agar containing 0,5% yeast extract. It was pre-selected 55 bacteria with different morphological characteristics, these bacteria were grown simultaneously in 5% salt water agar containing 1% tributyrin, Tween 80 and olive oil. It was selected 14 bacteria based on the ability to hydrolyze specific substrates and shown high salt tolerance rate between 0 to 25%. These bacteria grew optimally at pH 7 and 37 °C, six were Gram positive and Gram negative eight, 12 bacteria hydrolyzed tributyrin, Tween 80 and olive oil, six produced amylases and proteases. The 14 halotolerant bacteria with lipolytic activity isolated from the Pilluana salterns are promising lipase production sources and oily waste bio-treatment.
16
artículo
En el presente trabajo evaluamos la actividad antibacteriana y antifúngica de actinomycetes marinos sobre patógenos de origen clínico. Asimismo, fueron evaluadas la capacidad de producir enzimas extracelulares como carbohidrasas, lipasas y proteasas. Los Actinomycetes fueron aislados de sedimentos colectados entre setiembre a diciembre del 2005 de las Bahías de Ancón (Lima) e Independencia (Ica) de 34 y 100 m de profundidad. El aislamiento se realizó en Agar Caseína - Almidón (ACA) y Agar Marino (AM) con adición de Cicloheximide (10 μg/mL). Las evaluaciones antimicrobianas fueron realizadas frente a bacterias patógenas antibiótico-multirresistentes y hongos de origen clínico; en tanto, para evaluar su actividad multienzimática se utilizaron sustratos poliméricos diversos. Se aislaron un total de 62 actinomycetes, de los cuales 31 (50%) mostraron actividad antibacteriana fr...
17
artículo
In the present research we evaluated the antibacterial and antifungical activity of marine actinomycetes over pathogen of clinical origin. Likewise, it was evaluated the capacity to produce extracellular enzymes like carbohidrases, lipases and proteases. The Actinomycetes were isolated from sediments collected between September to December 2005 of Ancón (Lima) and Independencia (Ica) Bays at depths of 34 and 100 m. The isolation was performed in Casein - Starch Agar (CSA) and Marine Agar (AM) with addition of Cicloheximide (10 μg/mL). The antimicrobial evaluations were done comparing them with pathogenic antibiotic-multiresistant bacteria and fungi from clinical origin; in as much, to evaluate their multienzimatic activity several polimeric substrates were used. A total of 62 actinomycetes were isolated, 31 of there (50%) showed antibacterial activity in opposite to Staphylococcus aure...
18
artículo
Publicado 2025
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Introducción: La expresión recombinante de toxinas de origen ofídico ha permitido potenciar su estudio tanto estructural y funcional, especialmente en sus aplicaciones biomédicas. Pictobina es una enzima similar a trombina del veneno de B. pictus, especie endémica del Perú, que presenta un alto potencial. Objetivo: producir la versión recombinante de Pictobina (rPictobina) en el modelo eucariótico Piccha pastoris, Métodos: Se inició con las síntesis comerciales de la secuencia nucleotídica de Pictobina (750 pb), que incorpora los sitios de restricción EcoR I y Not I. Esta secuencia fue amplificada por PCR, insertada en el vector pPICZα-C y clonada en E. coli OneShot TOP10. Posteriormente, se amplificó el plásmido pPICZα-C–rPictobina para su transformación y expresión en el sistema Pichia pastoris GS115. Resultados: La expresión se logró tras la inducción con metan...
19
tesis de grado
Publicado 2021
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Las proteínas Clp son un grupo de proteasas caseolíticas del grupo serina dependiente de ATP. Su función principal es el control de calidad de las proteínas. En Mycobacterium tuberculosis, ClpC1 participa del mecanismo de acción de fármacos antituberculosos como pirazinamida (PZA) por ello es importante estudios sobre su función y estructura. Asimismo, monómero de ClpC1 de Bacillus subtilis se ha cristalizado completamente por ello se utiliza como plantilla para modelar ClpC1 de otras bacterias. ClpC1 es una proteína citosólica por lo que su expresión en forma soluble ha sido confirmada en estudios previos. El sistema de expresión en Escherichia coli usando el plásmido pET28a(+) como vector de expresión, ha sido utilizado para producir ClpC1; sin embargo, no hay información acerca del porcentaje de solubilidad y concentración de proteína producida. Por otro lado, el plá...
20
tesis de maestría
Publicado 2024
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Universidad Nacional Agraria La Molina. Escuela de Posgrado. Maestría en Acuicultura
