The banana (Musa AAA) is affected by Fusarium oxysporum f. sp. cubense, which causes discoloration in the xylem duct, leading to terminal wilting. The use of plant growth promoting rhizobacteria (PGPR) as a biological control produces different antagonistic compounds and inhibits the growth of various phytopathogens. The objective of the study was based on the molecular identification of rhizobacteria that produce phytohormones with biocontrol activity against Foc-R1. The presence of the 225 bp ChiA gene was observed in PGPR. Phylogenetic analysis of 16S rRNA by sequencing and ERIC-PCR showed genetic variability with the formation of four subgroups. Molecular identification by sequencing the 16S rRNA gene defined the genera as Klebsiella, Enterobacter, and Pseudomonas. There is variation in the biosynthesis of the phytohormones AIA, AG, and AS in strains MH-18, W-417, and FZ 9-7 at 72 h....

Meloidogyne incognita root-knot nematode is one of the main causes of tomato root damage and consequently crop production losses. Thus, in in vitro conditions, the number of nematodes hatched eggs (%) at 4 and 6 days and nematode mortality (J2 stage) at 8, 18, and 24 h, were evaluated in Petri dishes containing the candidate rhizobacteria Enterobacter asburiae (BA4-19 and PM3-14), Acinetobacter calcoaceticus (BM2-12), Klebsiella variicola (BO3-4) and Serratia marcescens (PM3-8). The well-known Pseudomonas protegens (CHA0) and P. veronii (R4) were used as controls. In greenhouse conditions, plant height, root weight, and symptoms, as well as gall and nematode numbers, were determined in tomato plants infected by M. incognita and treated with the seven rhizobacteria. In addition, all variables were correlated using Pearson's analysis. In general, a significant correlation was observed amon...

The objective of the work focused on characterizing the biodiversity of Phytophthora spp. and rhizobacteria with potential for biocontrol in black pod disease in CCN-51. Nine plant samples with symptoms of the disease were collected from cocoa-producing areas, evaluating their morphological characteristics and identification by PCR. Different morphotypes of colonies are observed: cottony, regular and starry edge characteristic for Phytophthora spp. Electrophoresis profiles reveal an amplicon of 159 bp for P. palmivora, demonstrating its distribution in the nine zones. The tests on mycelial inhibition in vitro to P. palmivora, determined high levels of antagonism of 78 and 60% by direct confrontation with the rhizobacteria. These results in bio-protection to CCN-51 by applications of P. veronii R4, A. calcoaceticus BMR2-12, S. marcescens PM3-8 and P. protegens CHA0 are effective in the co...

The objective of the work focused on characterizing the biodiversity of Phytophthora spp. and rhizobacteria with potential for biocontrol in black pod disease in CCN-51. Nine plant samples with symptoms of the disease were collected from cocoa-producing areas, evaluating their morphological characteristics and identification by PCR. Different morphotypes of colonies are observed: cottony, regular and starry edge characteristic for Phytophthora spp. Electrophoresis profiles reveal an amplicon of 159 bp for P. palmivora, demonstrating its distribution in the nine zones. The tests on mycelial inhibition in vitro to P. palmivora, determined high levels of antagonism of 78 and 60% by direct confrontation with the rhizobacteria. These results in bio-protection to CCN-51 by applications of P. veronii R4, A. calcoaceticus BMR2-12, S. marcescens PM3-8 and P. protegens CHA0 are effective in the co...

The objective of the work was focused on studying the biodiversity of ecotypes and aggressiveness ranges of Moniliophthora perniciosa, in Theobroma cacao. Plant material with the presence of the pathogen from 12 plantations of T. cacao L Nacional was sampled, analyzing its morphological characteristics, identification by PCR and the aggressiveness of the disease in national cacao EET-19. The basidiocarps differ in size and basidiospore production considering the strain MpLRBF-105 with the largest diameter of basidiocarp and basidiospore discharge. The basidiocarps differ in size and basidiospore production considering the strain MpLRBF-105 with the largest diameter of basidiocarp and basidiospore discharge. The content of lamelulas would be adding the increase of basidiospores in the strains (MpLRBF105 and MpBOLN107), with the largest dimensions between lengths of (11.58 to 12.01 µm) an...