A coagulant enzyme from Bothrops brazili snake venom called thrombin-like enzyme was purified by three successive chromatographic steps on Sephadex G-75, DEAE Sephadex A-50 and Sephadex G-50 using 0.05M Tris-HCl buffer pH 8.5. The enzyme was purified 15.9 times with a yield of 28.6% and by PAGE-SDS a single protein band of 48 kDa was obtained both in reducing and non-reducing conditions using 2β-Mercaptoethano., It is a unicatenaryprotein with coagulant activity on both citrated human plasma and bovine fibrinogen. The enzyme showed amidolytic activity on the chromogenic substrate Benzoyl-Arginyl-pNitroaniline (BApNA) and the coagulant potency on bovine fibrinogen was calculated on 121 NIH units of thrombin / mg. The enzyme was inhibited by PMSF and the soybean trypsin inhibitor, therefore, it is a serine protease; the optimum pH for the amidolytic activity was 8.5 and the protein was st...

The objective of this study was to evaluate the elaboration of a salami that contains a natural colorant extracted from the seeds of ayrampo (Opuntia soehrensii), using an extract of this natural product at different concentration levels and using microbiological and sensorial characteristics as acceptance criteria of the final product. Initially, a physicochemical characterization of the seeds was carried out, which showed the high nutritional value of this natural product and its antioxidant power. In an experiment aimed at determining the effect of the concentration of the ayrampo seed extract on the quality of salami, it was shown that for extract concentrations between 150 and 250 g / L of water in the meat mass, there are no significant differences in terms of the characteristics of smell, color, taste and appearance of the final product. This was sensory tested through a 9-point h...

El trabajo se desarrolló entre setiembre y noviembre del 2010, teniendo como objetivo evaluar la influencia de algunos factores ambientales del agua sobre la presencia de parásitos en alevinos de Arapaima gigas “paiche” en estanques de cultivo de la Piscigranja Quistococha – UNAP, de donde se analizaron 20 ejemplares con peso y longitud promedio de 96.80±35.94 gramos y 24.57±3.04 centímetros. El sacrificio de los peces fue a razón de 2 por semana y se llevó a cabo realizando una perforación a la altura de la región cefálica (fontanela). El análisis de las estructuras de las muestras fue a nivel de piel, aletas, branquias, fosas nasales, órganos internos y músculo. Los índices parasitarios aplicados fueron prevalencia, intensidad, intensidad media, abundancia y abundancia media, lugar de fijación y estatus comunitario, índice de dispersión y tipo de distribución. E...

The present study was carried out between January and May 2023, with the purpose of analyzing the current state of the consumer fishery in the Loreto region; For this purpose, statistical information on fishing landing volumes for the period 2010 – 2022 was used. The results show a total record of 142,193.50 ± 3,827.90 t, registering Iquitos as the area with the highest landing volume (60.33%); Likewise, Caballococha and El Estrecho show an upward trend line in landing volumes; Regarding the state of conservation of the fishing resource, 88.98% were landed in the fresh state, 2.92% in the salted state and 8.10% in the dry-salted state; In relation to the species with the highest landing volume, these are grouped in the Order Characiformes (75.47%), with Prochilodus nigricans being the one with the highest capture record with 26.62%, followed by Mylossoma albiscopum (13.33%); These cat...

The objective of this study was to evaluate the elaboration of a salami that contains a natural colorant extracted from the seeds of ayrampo (Opuntia soehrensii), using an extract of this natural product at different concentration levels and using microbiological and sensorial characteristics as acceptance criteria of the final product. Initially, a physicochemical characterization of the seeds was carried out, which showed the high nutritional value of this natural product and its antioxidant power. In an experiment aimed at determining the effect of the concentration of the ayrampo seed extract on the quality of salami, it was shown that for extract concentrations between 150 and 250 g / L of water in the meat mass, there are no significant differences in terms of the characteristics of smell, color, taste and appearance of the final product. This was sensory tested through a 9-point h...

Los pocos estudios para la elaboración de un plan de manejo de residuos sólidos y escasas normas, guías nacionales para su elaboración, hacen propicio la elaboración del presente trabajo. Este estudio permitirá proponer conjunto de procedimientos y estrategias para el manejo de los residuos generados en la empresa de estampados. Se tiene como objetivo elaborar una propuesta de plan de minimización y manejo de residuos sólidos considerando un enfoque cuali-cuantitativo de diseño no experimental planteando como metodología tres fases sistemáticas que son: 1. Evaluación preliminar, el cual consiste en el desarrollo de una inspección técnica y entrevista al personal clave con el fin de conocer la situación actual de la empresa en cuanto al manejo de sus residuos, 2. Caracterización, regido por la guía de caracterización de residuos sólidos municipales del ministerio del am...

El proyecto busca la elaboración de un estudio de pre factibilidad para la fabricación de cubiertos biodegradables a partir de cascarilla de arroz con la finalidad de conocer la posibilidad de incursionar en el mercado peruano. Va enfocado a hombres y mujeres entre los 18 a 55 años de edad, correspondiente al nivel socioeconómico A, B y C de las zonas 4, 6 y 7 de Lima Metropolitana. Lo que se espera en un futuro, es ser una compañía reconocida con una cultura ecológica y comprometida con la sostenibilidad del ambiente.

En la actualidad, todo profesional en cualquier campo del conocimiento debe tener las competencias indispensables para manejar un volumen enorme de datos de tipo cuantitativo y cualitativo, y aplicar las técnicas y/o métodos estadísticos que lo ayuden en el proceso de toma de decisiones. Estadística descriptiva y probabilidad ha sido especialmente escrito con un enfoque por competencias para que el estudiante adquiera el conocimiento y desarrolle capacidades complejas a través de la acción en diversos ámbitos, que le permita tomar las decisiones correctas.

El estudio tuvo por objeto conocer cómo se desarrolla la gestión de los residuos sólidos en el derecho de tener un ambiente saludable de los ciudadanos. Tuvo una metodología descriptiva simple, con enfoque cualitativo en que como muestra se tuvo a 9 artículos de revista científicas indexadas de los cinco últimos años, aplicando la revisión sistemática como técnica y la guía de revisión como instrumento. Dentro de los principales resultados se encontró que, el 60% de las investigaciones indicaron la relación directa entre la gestión de los autoridades con la gestión de residuos sólidos y en la mayoría de países de Latino América todo el proceso es deficiente, por no tener herramientas administrativas para formular e implementar proyectos articuladores con las empresas y población para disminuir la contaminación con desechos sólidos, esto se suma la escasa voluntad ...

The gold extraction method with α-cyclodextrin in the mineral sample was applied to the study sample, then the incidence in the reduction of occupational accidents and negative environmental impacts was determined and the problems were raised. α-cyclodextrin in the extraction of gold in ore influences the reduction of occupational accidents and negative environmental impacts? To what extent does the realization of the hazard identification and risk assessment matrix, and calculate the percentage of occupational risk of the method of gold extraction with α-cyclodextrin can lead to occupational accidents? and To what extent can carrying out the matrix for the identification of aspects and evaluation of environmental impacts, and calculating the percentage of the environmental risk index of the gold extraction method with α-cyclodextrin, can generate environmental impacts?. The method w...

In the present investigation it will benefit the rural population of the High Andean communities, that adopt biogas and biofertilizer as alternative energy and organic fertilizer, respectively; As they will have at their disposal an ecological fuel and organic fertilizer throughout the year, improving their quality of life.For this purpose, tubular biodigesters of PVC geomembrane of 8 and 12 m3 of total volume (1.27 m in diameter and 6.7 to 10 m in length) were used, with 6 and 9 m3 of working volume, respectively, occupied By the manure mixture of cattle: water in the proportion 1: 5. Biodigesters were installed in the Andean communities.Ancash. Peru. Based on the results obtained, at 38 days of loading the biodigester, the biogas produced reached a pressure of 10 cm of water throughout the system. For all this activity, it was required an operating time of the kitchen to biogas of two ...

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Based on the need to make accurate decisions in the face of the COVID-19 pandemic in Peru, pandemic in Peru, specifically for the recovery of the tourism sector. For this purpose, a characterization of the behavior of the pandemic was carried out in the first three months of its development, based on the analysis of trends and the determination of the effective reproduction number (Rt) from statistical-mathematical methods. A variant of the SIR mathematical model was applied to forecast the possible evolution of the pandemic. This model was adjusted with the GlobalSearch optimization strategy of the MATLAB software. His solution used the MATLAB function ode23tb, which uses a combined Runge-Kutta algorithm with a trapezoidal rule algorithm. With the application of the Kaizen strategy as a means of continuous improvement, a set of actions were proposed that could be carried out today and t...