The study was carried out to evaluate the effect of four seminal plasma dilutions on ovulation rate and corpus luteum development in alpacas. Forty four non-lactating alpacas with the presence of a >7 mm dominant follicle detected by transrectal ultrasonography were selected. The animals were randomly distributed in five experimental groups: G1 (n=10) seminal plasma (SP) diluted 1:1 (v/v) with phosphate buffered saline (PBS); G2 (n=10) SP diluted 1:2 (v/v) with PBS; G3 (n=10) SP diluted 1:4 (v/v) with PBS; G4 (n=10) SP 1:8 (v/v) with PBS; and G5 (n=4) control group with PBS. A dose of 1.5 ml of the respective solution was intramuscularly administrated. Ovulation rate and corpus luteum size were evaluated at second and ninth day respectively (D0= day of treatment) through ultrasonography. Blood samples were taken on D0, D3, D6, D9 and D12 to determine serum progesterone levels by radio...

The study was carried out to evaluate the effect of four seminal plasma dilutions on ovulation rate and corpus luteum development in alpacas. Forty four non-lactating alpacas with the presence of a >7 mm dominant follicle detected by transrectal ultrasonography were selected. The animals were randomly distributed in five experimental groups: G1 (n=10) seminal plasma (SP) diluted 1:1 (v/v) with phosphate buffered saline (PBS); G2 (n=10) SP diluted 1:2 (v/v) with PBS; G3 (n=10) SP diluted 1:4 (v/v) with PBS; G4 (n=10) SP 1:8 (v/v) with PBS; and G5 (n=4) control group with PBS. A dose of 1.5 ml of the respective solution was intramuscularly administrated. Ovulation rate and corpus luteum size were evaluated at second and ninth day respectively (D0= day of treatment) through ultrasonography. Blood samples were taken on D0, D3, D6, D9 and D12 to determine serum progesterone levels by radio...

El estudio tuvo como objetivo evaluar el efecto de cuatro diluciones de plasma seminal sobre la tasa de ovulación y formación de cuerpo lúteo en alpacas. Se seleccionaron 44 alpacas sin cría al pie y con la presencia de un folículo dominante >7 mm detectado por ecografía transrectal. Los animales se distribuyeron al azar en cinco grupos experimentales: G1, plasma seminal (PS) diluido 1:1 (v/v) con fosfato salino bufferado (PBS); G2, PS diluido 1:2 (v/v) con PBS; G3, PS diluido 1:4 (v/v) con PBS; G4, PS diluido 1:8 (v/v) con PBS; y G5, grupo control con PBS. Se aplicó 1.5 ml de la dilución correspondiente por vía intramuscular. La tasa de ovulación y el tamaño de cuerpo lúteo fueron evaluados al segundo y noveno día, respectivamente (D0= inicio de tratamiento) a través de ecografía transrectal. Muestras de sangre fueron tomadas los días D0, D3, D6, D9 y D12 para determinar...

The aim of this study was to characterize 20 strains of Salmonella enterica at molecular level and antimicrobial resistance. Of these, 15 strains were obtained from infected guinea pigs and five from clinically healthy guinea pigs from two intensive production centers located in Lima, Peru. The invA, prot6E and fliC genes corresponding to the genus Salmonella and serovars Enteritidis and Typhimurium, respectively, were detected by a multiple PCR technique. Genetic variability was detected using the BOX-PCR technique using the first BOXA1R. Resistance was evaluated using the Kirby Bauer technique based on erythromycin, nitrofurantoin, streptomycin, penicillin, enrofloxacin, fosfomycin, amoxicillin with clavulanic acid, sulfatrimetoprim and ciprofloxacin. Serotype Typhimurium was determined in 100% of the isolates. The evaluation of the electrophoretic profiles obtained by the BOX-PCR tech...

El objetivo del estudio fue caracterizar 20 cepas de Salmonella enterica a nivel molecular y de resistencia antimicrobiana. De estas, 15 fueron obtenidas de cuyes infectados y cinco de cuyes clínicamente sanos, procedentes de dos centros de producción intensiva ubicados en Lima, Perú. Mediante una técnica de PCR múltiple se detectaron los genes invA, prot6E y fliC, correspondientes al género Salmonella y serovares Enteritidis y Typhimurium, respectivamente. Se detectó la variabilidad genética mediante la técnica BOX-PCR utilizando el primer BOXA1R. La resistencia fue evaluada utilizando la técnica de Kirby Bauer en base a eritromicina, nitrofurantoína, estreptomicina, penicilina, enrofloxacina, fosfomicina, amoxicilina con ácido clavulánico, sulfatrimetoprim y ciprofloxacina. Se determinó la serovariedad Typhimurium en el 100% de los aislados. La evaluación de los perfiles...

The aim of this study was to evaluate the detection capacity of the Multiplex Polymerase Chain Reaction (PCR) method from non-selective pre-enrichment samples (using target sequences of InvA, fliC and prot6E genes) to diagnose Salmonella Typhimurium and Enteritidis and to determine the concordance between the conventional microbiological detection method which consist of non-selective pre-enrichment, selective enrichment, differential agar isolation and biochemical tests. A total of 111 liver samples from guinea pigs with presumptive diagnosis of salmonellosis, collected in Chancay (Lima) and El Mantaro (Junín), Peru were analyzed. Salmonella Typhimurium was detected by multiplex PCR in 54% (60/111) of the samples and by microbiological analysis in 41% (45/111). A substantial concordance was found with a Kappa value of 0.64. The McNemar test showed that the results of both tests were st...

The aim of this study was to evaluate the detection capacity of the Multiplex Polymerase Chain Reaction (PCR) method from non-selective pre-enrichment samples (using target sequences of InvA, fliC and prot6E genes) to diagnose Salmonella Typhimurium and Enteritidis and to determine the concordance between the conventional microbiological detection method which consist of non-selective pre-enrichment, selective enrichment, differential agar isolation and biochemical tests. A total of 111 liver samples from guinea pigs with presumptive diagnosis of salmonellosis, collected in Chancay (Lima) and El Mantaro (Junín), Peru were analyzed. Salmonella Typhimurium was detected by multiplex PCR in 54% (60/111) of the samples and by microbiological analysis in 41% (45/111). A substantial concordance was found with a Kappa value of 0.64. The McNemar test showed that the results of both tests were st...