The objective of the present research was to determine the presence and the association between rotavirus, biotype E. coli and fimbriated E. coli in the enteric infections of neonatal alpacas under natural conditions. The detection of rotavirus was carried out by agglutination with monoclonal antibodies, the biotyping of E. coli by API 20E system and the detection of fimbrial antigens by agglutination using monoclonal antibodies. In 15 out of 160 (9.4%) of fecal samples from neonatal alpacas with enteric infection was detected rotavirus. Ten biotypes of E. coli was found and 26% of the E. coli isolates from alpaca neonates with enteric infection had the F41 antigen in contrast with 48% from clinically healthy neonatal alpacas. Rotavirus was found in association with 18.8% of the E. coli isolates identified as biotypes A and C. The 57.8 % of E. coli isolates from neonatal alpacas with ent...

El objetivo del presente trabajo fue determinar la presencia y la asociación entre rotavirus y la Escherichia coli fimbriada como agentes patógenos causantes de las infecciones entéricas en condiciones naturales en alpacas neonatas. La detección de rotavirus y de antígenos fimbriales se realizó mediante aglutinación con anticuerpos monoclonales embebidos en látex y la biotipificación se realizó mediante el sistema API 20E. En el 9.3% de las muestras de heces se detectó rotavirus. Se encontró 10 biotipos de E. coli, donde se detectó un 26% de antígeno F41 en alpacas neonatas con infección entérica en contraste con el 48% en los aislamientos de E. coli de alpacas clínicamente sanas. Rotavirus se encuentra en asociación con el 18.8% de los aislamientos de E. coli identificados como biotipos A y C. El 57.8% de aislamientos de E. coli de alpacas neonatas con infección enté...

This study aimed to determine the seroprevalence of antibodies against Brucella canis in dogs of the district of Pucusana, Lima, Peru. Blood samples from 202 dogs older than 6 months of age were collected, regardless of breed, sex or reproductive history. The sera was analysed by the technique of agar gel immunodiffusion (AGID). The results showed 21.3 ± 2.9% (43/202) of dogs seropositive for antibodies against B. canis. The variables sex, breed, occurrence of abortion and street presence were evaluated resulting significant association between seropositivity to B. canis and sex (p<0.05).

The study evaluated the seroprevalence of canine brucelosis in the district of Los Olivos, Lima, Peru by the Agar Gel Immunodifussion test (AGID). Blood samples (n=288) were collected from dogs older than 2 months of age. The sera were tested for Brucella canis. The seroprevalence of canine brucelosis was 4.9 ± 1.8% (95% CI) and the corrected seroprevalence was 5.3 ± 2.6% (95% CI), without significant association for age, sex, physical condition, indoor/outdoor habits and reproductive history.

El presente estudio tuvo como objetivo determinar la seroprevalencia de anticuerpos contra Brucella canis en perros del distrito de Pucusana, Lima, Perú. Se colectaron 202 muestras de sangre de perros mayores a 6 meses de edad de diversos grupos raciales, sexo e historial reproductivo. El análisis de los sueros se realizó mediante la técnica de inmunodifusión en gel de agar (IDGA). El 21.3 ± 2.9% (43/202) de los perros resultó seropositivo a anticuerpos contra B. canis. Se evaluaron las variables sexo, raza, ocurrencia de abortos y presencia en las calles, encontrándose asociación significativa entre la seropositividad a B. canis y el sexo (p<0.05).

El estudio evaluó la seroprevalencia de brucelosis canina en el distrito de Los Olivos, Lima, Perú, mediante el método diagnóstico de Inmunodifusión en Gel de Agar (IDGA). Se recolectaron 288 muestras de sangre de canes mayores de dos meses de edad. Los sueros resultantes se analizaron para la determinación de seropositividad a Brucella canis. La seroprevalencia de brucelosis canina fue de 4.9 ± 1.8% (IC 95%) y una seroprevalencia corregida de 5.3 ± 2.6% (IC 95%), sin encontrar un efecto significativo para las variables edad, sexo, condición fisiológica, hábitos de paseo e historia reproductiva.

The paper document three cases occurred in May and June, 2006, based on lung samples from pigs of two farms and one slaughterhouse in Lima, Peru. The results of isolation and biochemical test were compatible with Haemophilus parasuis.

The present study aimed to determine the antimicrobial susceptibility of Escherichia coli isolates in neonatal alpacas with and without diarrhea in peasant communities in Lachocc, Huancavelica, Peru. Rectal swabs from alpacas with diarrhoea (n=95) and from apparently normal alpacas (n=60) of 1 to 2 months of age were analysed. The results showed that 48.4% (46/95) and 60% (36/60) of samples from animals with and without diarrhea were positive to E. coli. The isolates were sensitive to most of the antibiotics, especially trimethoprim (98%), gentamicin (95%) and fosfomycin (88%). The exception was nitrofurantoin, whose strains showed low susceptibility (8.3%).

Several genera and species of nonhuman primates can be found in Peru. Among them, Ateles belzebuth, Ateles chamek, Callicebus oenanthe, Lagothrix cana and Lagothrix lagotricha. These species according to the IUCN Red List are in the threatened category. The aim of this study was to determine the antibacterial sensitivity profile of enteric isolates from Ateles, Callicebus and Lagothrix raised in semi-captivity. Sampling was performed by rectal swab in 56 nonhuman primates in the Center for Rescue and Rehabilitation of Primates Ikama Peru. Isolation and bacterial identification was performed using a standard protocol and bacterial sensibility by the Kirby-Bauer technique. A total of 106 bacterial strains of the family Enterobacteriaceae were isolated, where the most prevalent species was Escherichia coli (45.5%, 45/106). The isolates showed greater resistance to cephalothin (46.2%), amoxi...

Se presentan tres casos ocurridos entre mayo y junio de 2006, en base a muestras de pulmón de cerdo procedentes de dos granjas de crianza porcina y de un camal de la zona de Lima. Los resultados del aislamiento y pruebas bioquímicas fueron compatibles con Haemophilus parasuis.

El estudio tuvo como objetivo determinar la susceptibilidad antibiótica de aislados de Escherichia coli en crías de alpaca con y sin diarrea de las comunidades campesinas de la localidad de Lachocc, departamento de Huancavelica, Perú. Se analizaron hisopados rectales de 95 animales con diarrea y de 60 aparentemente sanos de 1 a 2 meses de edad. El 48.4% (46/95) y el 60% (36/60) de las muestras de animales con y sin diarrea fueron positivas al cultivo de E. coli. Estos aislados fueron sensibles a la mayoría de los antibacterianos, especialmente trimetoprim (98%), gentamicina (95%) y fosfomicina (88%). La excepción fue la nitrofurantoína, cuyas cepas mostraron una baja susceptibilidad (8.3%).

El Perú cuenta con diversos géneros y especies de primates no humanos, dentro de los que se encuentran las especies Ateles belzebuth, Ateles chamek, Callicebus oenanthe, Lagothrix cana y Lagothrix lagotricha cuyos estados de conservación según la Lista Roja de la UICN se encuentran en la categoría amenazada. El objetivo del presente estudio fue determinar el perfil de resistencia antibacteriana de cepas entéricas aisladas de Ateles, Callicebus y Lagothrix en semicautiverio en un centro de rescate, Perú. La toma de muestras se realizó por hisopado rectal en 56 primates no humanos habitantes del Centro de Rescate y Rehabilitación de Primates Ikama Peru. El aislamiento e identificación bacteriana se realizó mediante un protocolo estándar, y la sensibilidad bacteriana mediante la técnica de Kirby-Bauer. Se aislaron 106 cepas bacterianas de la familia Enterobacteriaceae. La espec...

The objective of this study was to determine the prevalence of canine brucellosis(Brucella canis) in two districts of the province of Callao. A total of 456 sera werecollected from dogs without distinction of breed, sex and age. Samples were analyzed bythe Agar Gel Immunodiffusion (AGID) test using B. ovis antigen. The results indicatedthat 15.6 ± 3.3 (71/456) of sampled animals were positive. No statistical differences werefound due to district, breed, sex or age; however, differences were found (p<0.05) beetweendogs with and without reproductive history (26.5 and 8.6% respectively).

The aim of the study was to determine the frequency of external parasites in guineapigs (Cavia porcellus) during the rainy and dry seasons in family-commercial farms inOxapampa, Pasco, Peru. Also, aimed to identify the ectoparasites and to determine theassociation between seasons and the presence of external parasites. A total of 230 animals were evaluated per season. Ectoparasites were collected using three techniques: skinscraping, transparent tape, and fine comb. The frequency of parasitism was 70.9 ± 5.9 and83.0 ± 4.9% for the rainy and dry seasons respectively. Five species of Acariformes andthree species of Phthiraptera were identified, and the most frequent were Chirodiscoidescaviae and Gliricola porcelli. Significant association was found between the season ofthe year and the presence of external parasitism (p<0.05).

The association between positive cases of Salmonella sp isolated from vaginal swabs collected within 24 hours of the parturition and stillbirth in a guinea pig farm in Huancayo, Peru was evaluated. The study included 258 breeding females using a CaseControl design, where litter size was paired between the two groups and parity and number of birth and barn of the breeding female were used as co-variables. In the ‘Case’ group were included females with at least one stillbirth per litter and in the ‘Control’ group were included females where the entire litter was alive. Results showed that 8.5% of the breeding females in the ‘Case’ group were positive to Salmonella sp, and by the logistic regression analysis was estimated an Odds Ratio of 4.32 (95% confidence interval; p<0.05) for positive cases in relation to the controls. On the other hand, the cause of death in 91.5% (118/...

A seroprevalence study on 5439 cattle from April to June 2007 was conducted based on a circumstantial diagnosis of two cases of bovine brucellosis in the district of Codo del Pozuzo, en the region of Huanuco. Two animals resulted positive to the Rose Bengal test but negative to the Complement Fixation test. The beta distribution using a simulation model showed a prevalence of 0.02% with a minimum interval of confidence of 0 and maximum of 0.06%.

The objective of the present study was to determine the sensitivity of Salmonella enterica to 12 antibiotics. The bacteria were isolated from guinea pigs showing clinical signs compatible to salmonellosis. Samples of spleen, liver and other organs showing pathological lesions were collected from unhealthy guinea pigs bred under a familiarcommercial system in the province of Carhuaz, Ancash between May to July 2007. Conventional methods were used in order to isolate S. enterica strains and the diffusion test of Kirby-Bauer method was used to evaluate the sensitivity to antibiotics. Strains of S. enterica were isolated from 61.5% of the animals. All of these strains (40/40) were sensible to enrofloxacin, sulfatrimethoprim, streptomycin, and amoxicillin, 97.5% to chloramphenicol and gentamicin, and 92.5% to fosfomicin. Resistant strains were found to furazolidone (15.0%) and colistin (12.5%...

El objetivo del estudio fue comparar la flora bacteriana de intestino y hepatopáncreas del caracol Helix aspersa Müller criados en dos sistemas de producción: intensivo y extensivo. Se colectaron 30 caracoles adultos aparentemente sanos por cada sistema de producción, en seis criaderos ubicados en la provincia de Lima. Se tomó muestras de mucosa intestinal y parénquima del hepatopáncreas de cada individuo, empleando protocolos de aislamientos establecidos para bacterias aerobias y aerobias facultativas. Se aisló bacterias de 15 géneros: Escherichia, Citrobacter, Klebsiella, Enterobacter, Serratia, Hafnia, Proteus, Providencia, Aeromonas, Staphylococcus, Streptococcus, Enterococcus, Micrococcus, Pseudomonas, Acinetobacter; y bacilos gramnegativos no fermentadores (BNF). Se obtuvo 193 cepas en las muestras de caracoles del sistema extensivo, donde el género Escherichia fue el de ...

En base a un diagnóstico circunstancial de dos casos de brucelosis en un hato bovino del distrito de Codo del Pozuzo, Huánuco, se llevó a cabo un estudio de prevalencia de Brucella sp. en 5439 bovinos entre abril a junio de 2007. Dos bovinos resultaron positivos a la prueba de Rosa de Bengala, pero negativos a la prueba confirmativa de Fijación del Complemento. El modelo de simulaciones estocásticas de distribución beta mostró una prevalencia de 0.02% con intervalo de confianza mínimo de 0 y máximo de 0.06%.

El objetivo del presente estudio fue determinar la sensibilidad de Salmonella enterica aislada de cuyes, con signos clínicos sugerentes de salmonelosis, frente a 12 antibacterianos. Los cuyes fueron criados bajo un sistema familiar-comercial en la provincia de Carhuaz, Áncash, entre mayo a julio de 2007. Se colectó muestras de bazo, hígado y otros órganos con lesión aparente (n=65). Para el aislamiento de S. enterica se utilizaron métodos convencionales y el análisis de sensibilidad a antibacterianos fue mediante el método de difusión por discos de Kirby-Bauer. Cepas de S. enterica s e aislaron en el 61.5% de los cuyes. El 100% de estas cepas (40/40) fue sensible a enrofloxacina, sulfatrimetoprim, estreptomicina y amoxicilina, el 97.5% al cloranfenicol y gentamicina, y el 92.5% a fosfomicina. Se encontraron cepas resistentes a furazolidona (15.0%) y colistina (12.5%) entre otra...