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Objective: To study the presence in two hospitals of Lima of extended spectrum β-lactamases (BLEE) and to identify TEM and SHV types produced by Escherichia coli and Klebsiella pneumoniae strains. Material and Methods: The selection and confirmation of BLEE producing strains was done by antimicrobial susceptibility tests. The identification of bla TEM and bla SHV genes was achieved by polymerase chain reaction (PCR) and DNA sequencing. Results: One hundred and thirty seven Escherichia coli and 18 Klebsiella pneumoniae strains were isolated from hospitalized patients between July and September 2000. Most showed high resistance to third-generation cephalosporins and aztreonam;2,9% of all E.coli and 44,4% of all K.pneumoniae isolates were confirmed as BLEE producer strains. All BLEE producer strains were multidrug-resistant and most presented coresistance to trimethoprim/sulfamethoxazole, ...
2
artículo
Publicado 2010
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Tuberculosis is a public health problem, generated by poverty, with high prevalence and incidence. The objetives of this study were to determine the associated factors to tuberculosis in a marginal urban health center of Lima. This is an observational study of case report undertaken in Chaclacayo since January 1999 to December 2008. The data collected was acquired from the routine reports of the National Control Program. A total of 197 cases were evaluated; 108 were men (55%) and 89 were women (45%). Within these 197 subjects, 151 were new cases (76.6%) and 32 were individuals who had been previously diagnosed with tuberculosis (16.2%). The incidence decreased from 305 x 100 000 in 1999 to 119 x 100 000 inhabitants in 2008. The more affected age groups were 15-29 years with 103 cases (52.3%). 174 patients showed a high prevalence of pulmonary tuberculosis (88%); successful treatment were...
3
artículo
Publicado 2010
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Tuberculosis is a public health problem, generated by poverty, with high prevalence and incidence. The objetives of this study were to determine the associated factors to tuberculosis in a marginal urban health center of Lima. This is an observational study of case report undertaken in Chaclacayo since January 1999 to December 2008. The data collected was acquired from the routine reports of the National Control Program. A total of 197 cases were evaluated; 108 were men (55%) and 89 were women (45%). Within these 197 subjects, 151 were new cases (76.6%) and 32 were individuals who had been previously diagnosed with tuberculosis (16.2%). The incidence decreased from 305 x 100 000 in 1999 to 119 x 100 000 inhabitants in 2008. The more affected age groups were 15-29 years with 103 cases (52.3%). 174 patients showed a high prevalence of pulmonary tuberculosis (88%); successful treatment were...
4
artículo
Publicado 2013
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In this study, was investigated the antistaphylococcal activity in vitro of ethanol and hydroalcoholic extracts of 3 peruvian medicinal plants: Juglans neotropica Diels (bark), Piper lineatum Ruiz & Pav. (leaves) and Terminalia catappa L. (leaves). The species were collected in the regions of Amazonas and Cajamarca, in Peru. Antistaphylococcal activity was assessed by microdilution method. The organisms used were bacteria Staphylococcus aureus ATCC 25923 and Staphylococcus epidermidis ATCC 12228. All extracts investigated showed significant activity against both bacteria with a minimum inhibitory concentration (MIC) of 125 to 500 μg/mL for Staphylococcus aureus, with increased activity of the ethanol extract of Juglans neotropica Diels, and of 250 to 500 μg/mL for Staphylococcus epidermidis, having greater activity the hydroalcoholic extract of Piper lineatum Ruiz & Pav. Phytoc...
5
artículo
Publicado 2013
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In this study, was investigated the antistaphylococcal activity in vitro of ethanol and hydroalcoholic extracts of 3 peruvian medicinal plants: Juglans neotropica Diels (bark), Piper lineatum Ruiz & Pav. (leaves) and Terminalia catappa L. (leaves). The species were collected in the regions of Amazonas and Cajamarca, in Peru. Antistaphylococcal activity was assessed by microdilution method. The organisms used were bacteria Staphylococcus aureus ATCC 25923 and Staphylococcus epidermidis ATCC 12228. All extracts investigated showed significant activity against both bacteria with a minimum inhibitory concentration (MIC) of 125 to 500 μg/mL for Staphylococcus aureus, with increased activity of the ethanol extract of Juglans neotropica Diels, and of 250 to 500 μg/mL for Staphylococcus epidermidis, having greater activity the hydroalcoholic extract of Piper lineatum Ruiz & Pav. Phytoc...