Objective: To characterize soluble proteins present in Lepidium peruvianum G. Chacon ‘maca’ root by both unidimensional and bidimensional electrophoresis. Design: Observational and cross-sectional type study. Setting: Alberto Guzman Barron Biochemistry and Nutrition Research Center, Faculty of Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru. Materials: Roots of Lepidium peruvianum G. Chacon ‘maca’ white, yellow and purple ecotypes, collected in Junin and obtained from Universidad Nacional del Centro del Peru. Methods: Soluble total proteins extraction with an antioxidant solution and characterization by both unidimensional and bidimensional electrophoresis were done. Main outcome measures: Soluble protein number, molecular weight and most abundant proteins’ isoelectrical points. Results: The unidimensional electrophoretic analysis showed predominance of two prote...

Objetivos: Investigar la epidemiología molecular y clínica de E. coli y K. pneumoniae productoras de beta lactamasas de espectro extendido (BLEE), de muestras procedentes de pacientes hospitalizados y de pacientes de la comunidad. Diseño: Descriptivo. institución: Centro de Investigación de Bioquímica y Nutrición, Facultad de Medicina, UNMSM; Servicio de Microbiología Instituto Nacional de Salud del Niño. Participantes: Muestra poblacional de Lima, de 31 personas. intervenciones: Extracción de ADN genómico de E. coli, amplificación de la región espaciadora intergénica entre los genes 16S y 23S con primers 1406f y 23Sr, detección por electroforesis en agarosa al 1,5% y tinción con bromuro de etidio. Se realizó el análisis de los patrones de huella de ADN mediante análisis del espaciador intergénico ribosomal (RISA). Para el agrupamiento jerárquico de los patrones se u...

Objective: To determine the sensitivity of the test tuberculosis polymerase chain reaction (TB-PCR) compared to fluorochrome auramine “O” staining in histological embedded tissues of pleural biopsy in diagnosis of pleural tuberculosis. Materials and Methods: We used 48 formalin-fixed paraffin-embedded tissue obtained from the archives of the Department of Pathology, Hospital San Jose del Callao, and Instituto Nacional de Enfermedades Neoplasicas. Thirty cases had clinical diagnosis of tuberculosis and 18 different diagnosis. Results: Twenty-nine of 30 cases having tuberculosis were TB-PCR positive. All 18 negative cases were TB-PCR negative. The sensibility and the negative predictive value of TBPCR were 96,7% and 94,7% respectively. On the other hand, the sensitivity and negative predictive value of AFB-Auramine were 58,6% and 56,7%. The positive predictive value was 100% in both ca...

Introduction. The increase in resistance rates in uropathogenic bacteria and their spread has become in a significant worldwide public health, becoming more important the spread of resistant isolates producing of extended spectrum ß-lactamases, which are useful the molecular characterization of plasmids and other genetic elements of the bacteria to establish the genetic relationship between them, to have relevant data that we can suggest the direction of propagation of this type of resistance. The object of research was to determine the plasmid profiles in isolates of Escherichia coli and Klebsiella pneumoniae producing of extended spectrum ß-lactamases from urine cultures of the National Institute of Child Health, Lima, Peru. Methods. Descriptive, observational cross-sectional study. Results. Were detected different electrophoretic patternsin the samples, obtained from 1-7 bands per s...

Objective: To study the presence in two hospitals of Lima of extended spectrum β-lactamases (BLEE) and to identify TEM and SHV types produced by Escherichia coli and Klebsiella pneumoniae strains. Material and Methods: The selection and confirmation of BLEE producing strains was done by antimicrobial susceptibility tests. The identification of bla TEM and bla SHV genes was achieved by polymerase chain reaction (PCR) and DNA sequencing. Results: One hundred and thirty seven Escherichia coli and 18 Klebsiella pneumoniae strains were isolated from hospitalized patients between July and September 2000. Most showed high resistance to third-generation cephalosporins and aztreonam;2,9% of all E.coli and 44,4% of all K.pneumoniae isolates were confirmed as BLEE producer strains. All BLEE producer strains were multidrug-resistant and most presented coresistance to trimethoprim/sulfamethoxazole, ...