The Beauveria bassiana filamentous fungus is the most biological control agent frequently used today due to its entomopathogenic activity and antagonist fungi capacity. B. bassiana infects the insects through the degradation of the chitin present in the cuticle, also; affects the cell wall of pathogenic fungi. This mechanism involves enzymatic activities and activation of Chitinase genes. Gene expression of the family 18.4 (XM_008603039.1) and CHIT1 (EU828354.1) of B. bassiana, were evaluated, by RT-qPCR at 0, 4 and 7 days. The assay consists of four media with different carbon sources: colloidal chitin 1.8%, laminarin 0.1%, pulverized of F. oxysporum 0.1% and pulverized leaves infected with P. variabilis 0.1%. Relative expression using actinin and tubulin as referential genes, showed that CHIT1 gene expression increased 1.87 ± 0.35 times on day 7 in the medium with F. oxysporum, while ...

The Beauveria bassiana filamentous fungus is the most biological control agent frequently used today due to its entomopathogenic activity and antagonist fungi capacity. B. bassiana infects the insects through the degradation of the chitin present in the cuticle, also; affects the cell wall of pathogenic fungi. This mechanism involves enzymatic activities and activation of Chitinase genes. Gene expression of the family 18.4 (XM_008603039.1) and CHIT1 (EU828354.1) of B. bassiana, were evaluated, by RT-qPCR at 0, 4 and 7 days. The assay consists of four media with different carbon sources: colloidal chitin 1.8%, laminarin 0.1%, pulverized of F. oxysporum 0.1% and pulverized leaves infected with P. variabilis 0.1%. Relative expression using actinin and tubulin as referential genes, showed that CHIT1 gene expression increased 1.87 ± 0.35 times on day 7 in the medium with F. oxysporum, while ...

The fungus Beauveria bassiana Vuill. is well known for its ability entomopathogenic but there is also references to be an antagonist fungus, one of the possible ways of their antagonism it is antibiosis as is known the presence of hydrolytic enzymes in their genome. The main enzymes expressed against phytopathogenic are β-1,3-glucanases, since the cell wall of the phytopathogenic like fungi and oomycetes consists mostly polymers of β-1,3 glucans. The expression of exo-1,3-beta glucanase [XM_008597142.1] and glycoside hydrolase family 55 [XP_008597332.1] genes of B. bassiana was evaluated by qPCR at days 0, 4 and 7, against liquid culture mediums with different carbon sources, 0.1% laminarin, 0.1% pulverized of F. oxysporum and 0.1% pulverized of leaves infested with P. variabilis. Among the genes analyzed the highest rate was found in the cultures with F. oxysporum to the glycoside hyd...

The fungus Beauveria bassiana Vuill. is well known for its ability entomopathogenic but there is also references to be an antagonist fungus, one of the possible ways of their antagonism it is antibiosis as is known the presence of hydrolytic enzymes in their genome. The main enzymes expressed against phytopathogenic are β-1,3-glucanases, since the cell wall of the phytopathogenic like fungi and oomycetes consists mostly polymers of β-1,3 glucans. The expression of exo-1,3-beta glucanase [XM_008597142.1] and glycoside hydrolase family 55 [XP_008597332.1] genes of B. bassiana was evaluated by qPCR at days 0, 4 and 7, against liquid culture mediums with different carbon sources, 0.1% laminarin, 0.1% pulverized of F. oxysporum and 0.1% pulverized of leaves infested with P. variabilis. Among the genes analyzed the highest rate was found in the cultures with F. oxysporum to the glycoside hyd...