El objetivo de esta investigación fue estudiar el crecimiento de Beauveria bassiana, cultivado en medios líquidos suplementados con medio mínimo más sustratos artificiales (glucosa, laminarina o quitina coloidal) y con medio mínimo más sustratos naturales provenientes de Eurysacca melanocampta o Peronospora farinosa, para lo cual se evaluó el número de esporas y la cantidad de biomasa. Para ello se utilizó la cepa de B. bassiana CCB-LE 262, la cual fue inoculada en diferentes medios líquidos y donde se evaluó su crecimiento durante las 24 h, 96 h y 216 h. Nuestros resultados indican que a las 216 h la mayor cantidad de esporas se obtuvo en el medio artificial suplementado con laminarina (ML), con 67,80 esporas x 106·mL-1, mientras que el mejor medio natural fue el suplementado con polvo de E. melanocampta (M ) con 18,53 Em esporas x 106mL-1. La mejor biomasa se encontró a la...

The Beauveria bassiana filamentous fungus is the most biological control agent frequently used today due to its entomopathogenic activity and antagonist fungi capacity. B. bassiana infects the insects through the degradation of the chitin present in the cuticle, also; affects the cell wall of pathogenic fungi. This mechanism involves enzymatic activities and activation of Chitinase genes. Gene expression of the family 18.4 (XM_008603039.1) and CHIT1 (EU828354.1) of B. bassiana, were evaluated, by RT-qPCR at 0, 4 and 7 days. The assay consists of four media with different carbon sources: colloidal chitin 1.8%, laminarin 0.1%, pulverized of F. oxysporum 0.1% and pulverized leaves infected with P. variabilis 0.1%. Relative expression using actinin and tubulin as referential genes, showed that CHIT1 gene expression increased 1.87 ± 0.35 times on day 7 in the medium with F. oxysporum, while ...

The Beauveria bassiana filamentous fungus is the most biological control agent frequently used today due to its entomopathogenic activity and antagonist fungi capacity. B. bassiana infects the insects through the degradation of the chitin present in the cuticle, also; affects the cell wall of pathogenic fungi. This mechanism involves enzymatic activities and activation of Chitinase genes. Gene expression of the family 18.4 (XM_008603039.1) and CHIT1 (EU828354.1) of B. bassiana, were evaluated, by RT-qPCR at 0, 4 and 7 days. The assay consists of four media with different carbon sources: colloidal chitin 1.8%, laminarin 0.1%, pulverized of F. oxysporum 0.1% and pulverized leaves infected with P. variabilis 0.1%. Relative expression using actinin and tubulin as referential genes, showed that CHIT1 gene expression increased 1.87 ± 0.35 times on day 7 in the medium with F. oxysporum, while ...

The fungus Beauveria bassiana Vuill. is well known for its ability entomopathogenic but there is also references to be an antagonist fungus, one of the possible ways of their antagonism it is antibiosis as is known the presence of hydrolytic enzymes in their genome. The main enzymes expressed against phytopathogenic are β-1,3-glucanases, since the cell wall of the phytopathogenic like fungi and oomycetes consists mostly polymers of β-1,3 glucans. The expression of exo-1,3-beta glucanase [XM_008597142.1] and glycoside hydrolase family 55 [XP_008597332.1] genes of B. bassiana was evaluated by qPCR at days 0, 4 and 7, against liquid culture mediums with different carbon sources, 0.1% laminarin, 0.1% pulverized of F. oxysporum and 0.1% pulverized of leaves infested with P. variabilis. Among the genes analyzed the highest rate was found in the cultures with F. oxysporum to the glycoside hyd...

The fungus Beauveria bassiana Vuill. is well known for its ability entomopathogenic but there is also references to be an antagonist fungus, one of the possible ways of their antagonism it is antibiosis as is known the presence of hydrolytic enzymes in their genome. The main enzymes expressed against phytopathogenic are β-1,3-glucanases, since the cell wall of the phytopathogenic like fungi and oomycetes consists mostly polymers of β-1,3 glucans. The expression of exo-1,3-beta glucanase [XM_008597142.1] and glycoside hydrolase family 55 [XP_008597332.1] genes of B. bassiana was evaluated by qPCR at days 0, 4 and 7, against liquid culture mediums with different carbon sources, 0.1% laminarin, 0.1% pulverized of F. oxysporum and 0.1% pulverized of leaves infested with P. variabilis. Among the genes analyzed the highest rate was found in the cultures with F. oxysporum to the glycoside hyd...

Se evaluaron las respuestas fisiológicas de Hibiscus rosa sinensis L. (Malvaceae) durante abril a junio del 2010. Cuatro plantas cultivadas sobre suelo agrícola introducido en el cerro “El Agustino”, distrito del Agustino, Lima, Perú se seleccionaron al azar. Durante el periodo de evaluación se observó un descenso de la temperatura del aire de 29,4ºC a 17,2ºC y la humedad relativa aumentó de 40,5% a 79,7%. La temperatura registrada en el área foliar mostró sólo un ligero descenso de 23,8ºC a 16,5ºC en comparación a la temperatura del aire. El suelo se caracterizó por ser arenoso con un pH de 7,8 y una conductividad eléctrica de 2,1 mS. Una sola vez se registró riego por inundación, el pH del agua de regadío fue de 8,4 y su conductividad de 0,4 mS. El contenido relativo de agua de las plantas disminuyó semanalmente de 55,3% a 33,8%, mientras el porcentaje de estoma...