The Beauveria bassiana filamentous fungus is the most biological control agent frequently used today due to its entomopathogenic activity and antagonist fungi capacity. B. bassiana infects the insects through the degradation of the chitin present in the cuticle, also; affects the cell wall of pathogenic fungi. This mechanism involves enzymatic activities and activation of Chitinase genes. Gene expression of the family 18.4 (XM_008603039.1) and CHIT1 (EU828354.1) of B. bassiana, were evaluated, by RT-qPCR at 0, 4 and 7 days. The assay consists of four media with different carbon sources: colloidal chitin 1.8%, laminarin 0.1%, pulverized of F. oxysporum 0.1% and pulverized leaves infected with P. variabilis 0.1%. Relative expression using actinin and tubulin as referential genes, showed that CHIT1 gene expression increased 1.87 ± 0.35 times on day 7 in the medium with F. oxysporum, while ...

The Beauveria bassiana filamentous fungus is the most biological control agent frequently used today due to its entomopathogenic activity and antagonist fungi capacity. B. bassiana infects the insects through the degradation of the chitin present in the cuticle, also; affects the cell wall of pathogenic fungi. This mechanism involves enzymatic activities and activation of Chitinase genes. Gene expression of the family 18.4 (XM_008603039.1) and CHIT1 (EU828354.1) of B. bassiana, were evaluated, by RT-qPCR at 0, 4 and 7 days. The assay consists of four media with different carbon sources: colloidal chitin 1.8%, laminarin 0.1%, pulverized of F. oxysporum 0.1% and pulverized leaves infected with P. variabilis 0.1%. Relative expression using actinin and tubulin as referential genes, showed that CHIT1 gene expression increased 1.87 ± 0.35 times on day 7 in the medium with F. oxysporum, while ...

Se realizó el manejo de la colección, creación de base de datos y actualización al sistema APG III (2009) a las muestras proporcionadas por el herbario de la Universidad Nacional Federico Villarreal. Se presentan 419 ejemplares distribuidos en 22 órdenes, 53 familias, 192 géneros y 262 especies, de las cuales 10 especies son endémicas para el Perú y pertenecen al departamento de Lima. La familia Asteraceae representa el 25% de las especies, las hierbas predominan con un 48%, 40 especies presentan propiedades medicinales y la mayoría de especies fueron colectadas entre los 0-500 m.