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1
artículo
Publicado 2018
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Probiotic bacteria in aquaculture represent a control alternative and prevention of pathogens, in addition, reduce the use of antibiotics. However, it is necessary to search for other groups of bacteria with similar or better properties, obtained even from the same source. The aim of the study was to identify molecularly lactic acid bacteria with probiotic properties isolated from the posterior intestine of Nile tilapia (Oreochromis niloticus). The study was carried out from November 2017 to March 2018 evaluating the antagonistic, proteolytic, amylolytic, and antibiotic sensitivity activities, and antagonistic to the following pathogens: Aeromonas hydrophila, Aeromonas veronii, Pseudomonas aeruginosa, Pseudomonas putida and Plesiomonas shigelloides. It was identified by sequencing the 16S rRNA gene that Enterococcus faecium (IP5-2a) and Pedioc...
2
artículo
Publicado 2018
Enlace
Enlace
Probiotic bacteria in aquaculture represent a control alternative and prevention of pathogens, in addition, reduce the use of antibiotics. However, it is necessary to search for other groups of bacteria with similar or better properties, obtained even from the same source. The aim of the study was to identify molecularly lactic acid bacteria with probiotic properties isolated from the posterior intestine of Nile tilapia (Oreochromis niloticus). The study was carried out from November 2017 to March 2018 evaluating the antagonistic, proteolytic, amylolytic, and antibiotic sensitivity activities, and antagonistic to the following pathogens: Aeromonas hydrophila, Aeromonas veronii, Pseudomonas aeruginosa, Pseudomonas putida and Plesiomonas shigelloides. It was identified by sequencing the 16S rRNA gene that Enterococcus faecium (IP5-2a) and Pedioc...
3
artículo
Publicado 2018
Enlace
Enlace
Probiotic bacteria in aquaculture represent a control alternative and prevention of pathogens, in addition, reduce the use of antibiotics. However, it is necessary to search for other groups of bacteria with similar or better properties, obtained even from the same source. The aim of the study was to identify molecularly lactic acid bacteria with probiotic properties isolated from the posterior intestine of Nile tilapia (Oreochromis niloticus). The study was carried out from November 2017 to March 2018 evaluating the antagonistic, proteolytic, amylolytic, and antibiotic sensitivity activities, and antagonistic to the following pathogens: Aeromonas hydrophila, Aeromonas veronii, Pseudomonas aeruginosa, Pseudomonas putida and Plesiomonas shigelloides. It was identified by sequencing the 16S rRNA gene that Enterococcus faecium (IP5-2a) and Pedioc...
4
artículo
Publicado 2018
Enlace
Enlace
Probiotic bacteria in aquaculture represent a control alternative and prevention of pathogens, in addition, reduce the use of antibiotics. However, it is necessary to search for other groups of bacteria with similar or better properties, obtained even from the same source. The aim of the study was to identify molecularly lactic acid bacteria with probiotic properties isolated from the posterior intestine of Nile tilapia (Oreochromis niloticus). The study was carried out from November 2017 to March 2018 evaluating the antagonistic, proteolytic, amylolytic, and antibiotic sensitivity activities, and antagonistic to the following pathogens: Aeromonas hydrophila, Aeromonas veronii, Pseudomonas aeruginosa, Pseudomonas putida and Plesiomonas shigelloides. It was identified by sequencing the 16S rRNA gene that Enterococcus faecium (IP5-2a) and Pedioc...
5
artículo
Publicado 2005
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The bacteriorhodopsin (bR) is a protein which plays a role as a proton pump when is stimulated by light. The bR is involved in ATP's synthesis through a process of transductíon, turning light energy into chemical energy(photosynthesis). The bR codífied by bop gene and located in the cell envelope, covers up to 50 % of the extreme haloarchaeas when the environment is anaerobic. This feature shows a maximum degree of adjustment to anaerobic conditions of halophilic microorganism. Since the bR functíon as photoreceptor was proposed, several studies have been done in order to elucidate its amino acid sequence, structural conformation, physicochemical properties and expression and gene regulation characteristics. The excellent bR's photochemical and thermodynamic properties make it a peculiar molecule and a candidate to biotechnological applications in biocomputation for example in optical...
6
artículo
Publicado 2005
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The bacteriorhodopsin (bR) is a protein which plays a role as a proton pump when is stimulated by light. The bR is involved in ATP's synthesis through a process of transductíon, turning light energy into chemical energy(photosynthesis). The bR codífied by bop gene and located in the cell envelope, covers up to 50 % of the extreme haloarchaeas when the environment is anaerobic. This feature shows a maximum degree of adjustment to anaerobic conditions of halophilic microorganism. Since the bR functíon as photoreceptor was proposed, several studies have been done in order to elucidate its amino acid sequence, structural conformation, physicochemical properties and expression and gene regulation characteristics. The excellent bR's photochemical and thermodynamic properties make it a peculiar molecule and a candidate to biotechnological applications in biocomputation for example in optical...
7
tesis doctoral
Publicado 2013
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Bacillus thuringiensis (B. thuringiensis) es una bacteria Gram positiva que forma un cristal paraesporal con actividad insecticida contra insectos de diferentesórdenes. Los bioinsecticidas fabricados con las esporas y cristales de B. thuringiensis son una alternativa frente a los pesticidas químicos sintéticos para el control de mosquitos que son vectores de enfermedades metaxénicas, como por ejemplo, malaria y dengue. Se caracterizaron 53 cepas nativas de B. thuringiensis, procedentes de suelos peruanos, de los departamentos de Junín, Lima (Huaral), Ica, Cuzco, Tacna, Arequipa, Chiclayo y Cajamarca; dos de ellas codificadas como Ica10 y Caj1 por su procedencia, presentaron actividad entomocida contra larvas de Aedes aegypti. Se observan cepas que presentan bandas (toxinas) con pesos moleculares en el órden de los 36 kDa y mayores a 116 kDa; otras cepas presentan bandas a 97 y 75 k...
8
tesis de grado
Publicado 2018
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Universidad Nacional Agraria La Molina. Facultad de Ciencias. Departamento Académico de Biología
9
artículo
Publicado 2020
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Introduction. Studies on the infection of Helicobacter pylori and the IL-1B gene play an important role within inflammation and its proinflammatory properties that promote defense against pathogens. Polymorphisms have been related to a higher production of IL-1β associated with hypochlorhydria and cancer development under H. pylori infection, so the objective of this study was to evaluate polymorphism in the +3954 region (IL- 1B C> T) of the interleukin IL1β gene. Method. the blood samples were donated and collected to be processed for the determination of H. pylori, by means of the Urease Test (Proindusquim), in the city of Villahermosa Tabasco, Mexico, separating positive and negative samples (control), the positive samples were analyzed by PCR and enzymatic digestion for polymorphic analysis in the +3954 region (IL-1B C> T). Results. 245 samples 78 cases of H. p...
10
artículo
Publicado 2020
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Introduction. Studies on the infection of Helicobacter pylori and the IL-1B gene play an important role within inflammation and its proinflammatory properties that promote defense against pathogens. Polymorphisms have been related to a higher production of IL-1β associated with hypochlorhydria and cancer development under H. pylori infection, so the objective of this study was to evaluate polymorphism in the +3954 region (IL- 1B C> T) of the interleukin IL1β gene. Method. the blood samples were donated and collected to be processed for the determination of H. pylori, by means of the Urease Test (Proindusquim), in the city of Villahermosa Tabasco, Mexico, separating positive and negative samples (control), the positive samples were analyzed by PCR and enzymatic digestion for polymorphic analysis in the +3954 region (IL-1B C> T). Results. 245 samples 78 cases of H. p...
11
artículo
Publicado 2008
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Objective: To predict by using bioinformatic tools Mycobacterium tuberculosis (MT) metabolic pathways under hypoxic conditions. Design: Biology analysis. Setting: Instituto de Química Biológica, Microbiología y Biotecnología Marco Antonio Garrido Malo Biological, Microbiologic and Biotechnologic Chemistry Institute, Faculty of Pharmacy and Biochemistry, UNMSM. Biologic material: Mycobacterium tuberculosis genes. Methods: The study began with the selection of 355 genes of MT H37Rv whose expression has been shown by other studies is induced by hypoxic conditions and 359 genes whose expression was repressed. Up and down expressed genes were comparatively analyzed, localizing genes of each group within the MT genome and predicting some physicochemical properties (isoelectric point and hydrophobic moment) for their protein products. In order to assign a metabolic or regulatory pathway to ...
12
artículo
Publicado 2025
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Introducción: Las actividades antropogénicas como la minería y el uso excesivo de fertilizantes de origen químico han ocasionado una acumulación de cadmio en campos agrícolas a niveles riesgosos para la seguridad alimentaria, exportación de cultivos y consumo local. Objetivo: Caracterizar la cepa SABJ-3 de Bacillus velezensis a través del análisis de sus propiedades como rizobacteria promotora de crecimiento vegetal, su tolerancia al cadmio para su potencial uso como bacteria remediadora de suelos y su identificación molecular. Metodología: Se realizaron ensayos de solubilización de fosfatos en agar Pikovskaya, fijación de nitrógeno en agar Ashby y producción de sideróforos en Agar CAS, además se determinó la tolerancia al cadmio a través de la siembra en caldo de cultivo suplementado con 100, 200 y 400 ppm del metal y la identificación molecular mediante la secuencia...
13
tesis de grado
Publicado 2010
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Dentro de la gama de especies de la selva peruana, se encuentran caracoles terrestres de la familia Megalobulimidae (Mollusca, Gastropoda), entre las que destacan Megalobulimus popelairianus, Megalobulimus huascari y Megalobulimus capillaceus, que tienen importancia económica por las propiedades nutricionales de su carne y cosméticas de su baba. Este trabajo tuvo por objetivo principal desarrollar perfiles de ADN de los Megalobulimidae de San Martín, que hagan posible su reconocimiento a nivel específico y estudiar su salud genético poblacional mediante la diversidad genética, todo ello para garantizar su uso sustentable y eventualmente certificar a estas especies en el biocomercio peruano. Se evaluaron 65 muestras biológicas que fueron colectadas en septiembre de 2007 y enero, febrero y marzo de 2008. Se realizó la extracción de ADN con el método del CTAB y cloroformo, alcohol...
14
tesis de grado
Publicado 2015
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Universidad Nacional Agraria La Molina. Facultad de Ciencias. Departamento Académico de Biología
15
tesis de grado
Publicado 2020
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Background: Rifampicin (RIF) resistance in Mycobacterium tuberculosis is frequently caused by mutations in the rpoB gene. These mutations are associated with a fitness cost, which can be overcome by compensatory mutations in other genes, among which rpoC may be the most important. Aims and objectives: We analyzed 469 Peruvian M. tuberculosis clinical isolates to identify compensatory mutations in rpoC/rpoA associated with RIF resistance. Materials and methods: The M. tuberculosis isolates were collected and tested for RIF susceptibility and spoligotyping. Samples were sequenced and aligned to the reference genome to identify mutations. By analyzing the sequences and the metadata, we identified a list of rpoC mutations exclusively associated with RIF resistance and mutations in rpoB. We then evaluated the distribution of these mutations along the protein sequence and tridimensional struct...
16
tesis doctoral
Publicado 2016
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Universidad Nacional Agraria La Molina. Escuela de Posgrado. Doctorado en Agricultura Sustentable
