Identification and assessment of glucose transporters SGLT1 and GLUT2 and incretin GLP-1 in the small intestine of guinea pigs (cavia porcellus)
Descripción del Articulo
The aim of this study was to identify and assess the presence of glucose transporters SGLT1 and GLUT2 and incretin GLP-1 in the small intestine of guinea pig (Cavia porcellus). Five animals of 90 days old were used. Samples from the duodenum, jejunum and ileum were collected after slaughtering to ca...
| Autores: | , , , , , , |
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| Formato: | artículo |
| Fecha de Publicación: | 2012 |
| Institución: | Universidad Nacional Mayor de San Marcos |
| Repositorio: | Revistas - Universidad Nacional Mayor de San Marcos |
| Lenguaje: | español |
| OAI Identifier: | oai:ojs.csi.unmsm:article/932 |
| Enlace del recurso: | https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/932 |
| Nivel de acceso: | acceso abierto |
| Materia: | SGLT1 GLUT2 GLP-1 intestino delgado cuyes small intestine guinea pigs |
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Identification and assessment of glucose transporters SGLT1 and GLUT2 and incretin GLP-1 in the small intestine of guinea pigs (cavia porcellus)IDENTIFICACIÓN Y EVALUACIÓN DE TRANSPORTADORES DE GLUCOSA SGLT1 Y GLUT2 Y LA INCRETINA GLP-1 EN INTESTINO DELGADO DE CUYES (Cavia porcellus).Anglas P., JuanCueva M., SergioVásquez C., MaríaLira M., BorisEspinoza B., JuanLucas L., JuanRodríguez G., JoséSGLT1GLUT2GLP-1intestino delgadocuyesSGLT1GLUT2GLP-1small intestineguinea pigsThe aim of this study was to identify and assess the presence of glucose transporters SGLT1 and GLUT2 and incretin GLP-1 in the small intestine of guinea pig (Cavia porcellus). Five animals of 90 days old were used. Samples from the duodenum, jejunum and ileum were collected after slaughtering to carry out immunohistochemistry assays. Expression of SGLT1, GLUT2 and GLP-1 was observed in all three portions of the small intestine, where SGLT1 showed the highest expression, with a strong staining intensity in the apical and medium section of the crypt-villus axis. GLUT2 showed a strong staining intensity throughout the crypt-villus axis in the jejunum. GLP-1 expression was indirectly analyzed by calculating the number of L cells per mm2 of intestinal mucosa, resulting that the jejunum had the largest number (8.46 ±1.16 cells/mm2) (p<0.05). The results suggest that SGLT1 glucose transporter would be the most important in the guinea pig, and unlike other species, GLP-1 may be mostly secreted by the jejunum.El presente trabajo tuvo por objetivo identificar y evaluar la presencia de los transportadores de glucosa SGLT1 y GLUT2 y la incretina GLP-1 en intestino delgado de cuyes. Se usaron cinco cuyes (Cavia porcellus) reproductores de 90 días de edad. Luego del sacrificio, se tomaron muestras de duodeno, yeyuno e íleon para el procesamiento inmunohistoquímico. Se pudo identificar la presencia de SGLT1, GLUT2 y GLP-1 en las tres porciones intestinales, siendo SGLT1 la proteína de mayor presencia, con un nivel de marcación fuerte en la parte apical y media del eje cripta-vellosidad. El GLUT2 mostró un nivel de marcación fuerte en todo el eje cripta-vellosidad en el yeyuno. Asimismo, la presencia del GLP-1 fue analizada indirectamente por el número de células L/mm2 de mucosa intestinal, encontrándose el mayor número en el yeyuno (8.46 ± 1.16 células L/mm2) (p<0.05). Los resultados indican que SGLT1 sería el transportador de glucosa más importante en el cuy y, que a diferencia de otras especies, la mayor secreción de GLP-1 sería en yeyuno.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2012-12-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/93210.15381/rivep.v23i4.932Revista de Investigaciones Veterinarias del Perú; Vol. 23 Núm. 4 (2012); 399-405Revista de Investigaciones Veterinarias del Perú; Vol. 23 No. 4 (2012); 399-4051682-34191609-9117reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/932/757Derechos de autor 2012 Juan Anglas P., Sergio Cueva M., María Vásquez C., Boris Lira M., Juan Espinoza B., Juan Lucas L., José Rodríguez G.https://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/9322020-03-19T20:57:56Z |
| dc.title.none.fl_str_mv |
Identification and assessment of glucose transporters SGLT1 and GLUT2 and incretin GLP-1 in the small intestine of guinea pigs (cavia porcellus) IDENTIFICACIÓN Y EVALUACIÓN DE TRANSPORTADORES DE GLUCOSA SGLT1 Y GLUT2 Y LA INCRETINA GLP-1 EN INTESTINO DELGADO DE CUYES (Cavia porcellus). |
| title |
Identification and assessment of glucose transporters SGLT1 and GLUT2 and incretin GLP-1 in the small intestine of guinea pigs (cavia porcellus) |
| spellingShingle |
Identification and assessment of glucose transporters SGLT1 and GLUT2 and incretin GLP-1 in the small intestine of guinea pigs (cavia porcellus) Anglas P., Juan SGLT1 GLUT2 GLP-1 intestino delgado cuyes SGLT1 GLUT2 GLP-1 small intestine guinea pigs |
| title_short |
Identification and assessment of glucose transporters SGLT1 and GLUT2 and incretin GLP-1 in the small intestine of guinea pigs (cavia porcellus) |
| title_full |
Identification and assessment of glucose transporters SGLT1 and GLUT2 and incretin GLP-1 in the small intestine of guinea pigs (cavia porcellus) |
| title_fullStr |
Identification and assessment of glucose transporters SGLT1 and GLUT2 and incretin GLP-1 in the small intestine of guinea pigs (cavia porcellus) |
| title_full_unstemmed |
Identification and assessment of glucose transporters SGLT1 and GLUT2 and incretin GLP-1 in the small intestine of guinea pigs (cavia porcellus) |
| title_sort |
Identification and assessment of glucose transporters SGLT1 and GLUT2 and incretin GLP-1 in the small intestine of guinea pigs (cavia porcellus) |
| dc.creator.none.fl_str_mv |
Anglas P., Juan Cueva M., Sergio Vásquez C., María Lira M., Boris Espinoza B., Juan Lucas L., Juan Rodríguez G., José |
| author |
Anglas P., Juan |
| author_facet |
Anglas P., Juan Cueva M., Sergio Vásquez C., María Lira M., Boris Espinoza B., Juan Lucas L., Juan Rodríguez G., José |
| author_role |
author |
| author2 |
Cueva M., Sergio Vásquez C., María Lira M., Boris Espinoza B., Juan Lucas L., Juan Rodríguez G., José |
| author2_role |
author author author author author author |
| dc.subject.none.fl_str_mv |
SGLT1 GLUT2 GLP-1 intestino delgado cuyes SGLT1 GLUT2 GLP-1 small intestine guinea pigs |
| topic |
SGLT1 GLUT2 GLP-1 intestino delgado cuyes SGLT1 GLUT2 GLP-1 small intestine guinea pigs |
| description |
The aim of this study was to identify and assess the presence of glucose transporters SGLT1 and GLUT2 and incretin GLP-1 in the small intestine of guinea pig (Cavia porcellus). Five animals of 90 days old were used. Samples from the duodenum, jejunum and ileum were collected after slaughtering to carry out immunohistochemistry assays. Expression of SGLT1, GLUT2 and GLP-1 was observed in all three portions of the small intestine, where SGLT1 showed the highest expression, with a strong staining intensity in the apical and medium section of the crypt-villus axis. GLUT2 showed a strong staining intensity throughout the crypt-villus axis in the jejunum. GLP-1 expression was indirectly analyzed by calculating the number of L cells per mm2 of intestinal mucosa, resulting that the jejunum had the largest number (8.46 ±1.16 cells/mm2) (p<0.05). The results suggest that SGLT1 glucose transporter would be the most important in the guinea pig, and unlike other species, GLP-1 may be mostly secreted by the jejunum. |
| publishDate |
2012 |
| dc.date.none.fl_str_mv |
2012-12-28 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/932 10.15381/rivep.v23i4.932 |
| url |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/932 |
| identifier_str_mv |
10.15381/rivep.v23i4.932 |
| dc.language.none.fl_str_mv |
spa |
| language |
spa |
| dc.relation.none.fl_str_mv |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/932/757 |
| dc.rights.none.fl_str_mv |
https://creativecommons.org/licenses/by-nc-sa/4.0 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/4.0 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
| publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
| dc.source.none.fl_str_mv |
Revista de Investigaciones Veterinarias del Perú; Vol. 23 Núm. 4 (2012); 399-405 Revista de Investigaciones Veterinarias del Perú; Vol. 23 No. 4 (2012); 399-405 1682-3419 1609-9117 reponame:Revistas - Universidad Nacional Mayor de San Marcos instname:Universidad Nacional Mayor de San Marcos instacron:UNMSM |
| instname_str |
Universidad Nacional Mayor de San Marcos |
| instacron_str |
UNMSM |
| institution |
UNMSM |
| reponame_str |
Revistas - Universidad Nacional Mayor de San Marcos |
| collection |
Revistas - Universidad Nacional Mayor de San Marcos |
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| repository.mail.fl_str_mv |
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1795238222117208064 |
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13.949927 |
Nota importante:
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
