The aim of this study was to determine the existence of sperm subpopulations according to the morphometry of llama sperm, using a CASA-Morph system. Semen was collected from four 4-6-year-old Q'ara llamas by electroejaculation four times per animal, at one week intervals. The volume, total motility, sperm concentration, percentage of live sperm and percentage of sperm with acrosomal reaction were determined. The morphometric parameters of the sperm were evaluated using the CASA-Morph, Integrated Semen Analysis System (ISAS®v1). The length, width, area, perimeter, ellipticity, elongation, regularity and rugosity of the llama sperm head were determined, as well as the width, area, and distance and angle of insertion of the midpiece of the spermatozoa. The morphometric variables were distributed in five main components (PCA) called elongation, length, circularity, width of the midpiece and...

La criopreservación afecta la integridad de los espermatozoides de verraco y su funcionalidad. El objetivo de este estudio fue evaluar el efecto de la funcionalidad de la membrana plasmática de espermatozoides criopreservados de verraco en muestras con diferentes porcentajes de gametos viables. Un total de 15 eyaculados fueron utilizados a los que se les aplicaron cuatro tratamientos donde se consideró el 100, 75, 50 y 25 % de espermatozoides viables. Antes de los procesos de criopreservación, los espermatozoides se incubaron durante 24 horas. Cada tratamiento se diluyó en TRIS-Y (Tris con yema de huevo) y los espermatozoides se almacenaron hasta llegar a los 5 ºC. Luego, se utilizó TRIS-Y-G-E (Tris con yema de huevo-glicerol-Equex) para la dilución y se utilizaron pajillas de 0,5 mL para envasar cada material de cada tratamiento. La congelación se realizó manual en nitrógeno ...

The aim of this study was to determine the existence of sperm subpopulations according to the morphometry of llama sperm, using a CASA-Morph system. Semen was collected from four 4-6-year-old Q'ara llamas by electroejaculation four times per animal, at one week intervals. The volume, total motility, sperm concentration, percentage of live sperm and percentage of sperm with acrosomal reaction were determined. The morphometric parameters of the sperm were evaluated using the CASA-Morph, Integrated Semen Analysis System (ISAS®v1). The length, width, area, perimeter, ellipticity, elongation, regularity and rugosity of the llama sperm head were determined, as well as the width, area, and distance and angle of insertion of the midpiece of the spermatozoa. The morphometric variables were distributed in five main components (PCA) called elongation, length, circularity, width of the midpiece and...

El objetivo del estudio fue evaluar el efecto de la funcionalidad de la membrana plasmática de espermatozoides criopreservados de verraco en muestras con diferentes porcentajes de gametos viables. Se utilizaron 15 eyaculados en cuatro tratamientos con 100, 75, 50 y 25% de espermatozoides viables. Previo a la criopreservación los espermatozoides se incubaron durante 24 h. Cada tratamiento se diluyó en TRISY (Tris con yema de huevo) y los espermatozoides se almacenaron a 5 ºC. Luego, se utilizó TRIS-Y-G-E (Tris con yema de huevo-glicerol-Equex) para la dilución y se envasaron en pajillas de 0.5 mL. Se congelaron en forma manual en nitrógeno líquido en dos etapas de temperatura. La descongelación de las pajillas se hizo a 37 ºC durante 20 s. La funcionalidad espermática fue evaluada pos-descongelación (30 y 150 min), utilizando [Merocianina 540 (M540) y Yo-Pro-1] en muestras inc...

High-Andean Creole cattle represent a genetic resource with significant adaptive value in high-altitude ecosystems. In this study, 151 Creole cows from the provinces of Canas and Quispicanchis (Cusco, Peru) were evaluated to characterize their morphometry, calculate zoometric indices, and establish functional biotypes. Ten morphometric parameters were measured, and eight structural indices were calculated. Four differentiated biotypes were identified through the multivariate analysis and hierarchical clustering of quantitative variables (BI, BII, BIII, and BIV). Morphological differences were statistically significant (p < 0.05) and associated with different productive orientations. Biotype I showed greater thoracic development and a compact structure, which is compatible with intermediate beefaptitude; Biotype II exhibited more elongated proportions and a lower weight, indicating a dair...