La criopreservación afecta la integridad de los espermatozoides de verraco y su funcionalidad. El objetivo de este estudio fue evaluar el efecto de la funcionalidad de la membrana plasmática de espermatozoides criopreservados de verraco en muestras con diferentes porcentajes de gametos viables. Un total de 15 eyaculados fueron utilizados a los que se les aplicaron cuatro tratamientos donde se consideró el 100, 75, 50 y 25 % de espermatozoides viables. Antes de los procesos de criopreservación, los espermatozoides se incubaron durante 24 horas. Cada tratamiento se diluyó en TRIS-Y (Tris con yema de huevo) y los espermatozoides se almacenaron hasta llegar a los 5 ºC. Luego, se utilizó TRIS-Y-G-E (Tris con yema de huevo-glicerol-Equex) para la dilución y se utilizaron pajillas de 0,5 mL para envasar cada material de cada tratamiento. La congelación se realizó manual en nitrógeno ...

The aim of this study was to determine the effect of papain treatment on the kinetics of llama sperm. Four 5-8-year-old Ch'aku male llamas were selected and semen was collected using an artificial vagina and a dummy. Four semen collections were made per animal with intervals of one week. The semen samples were separated into two aliquots. One for fresh analysis and the other to be treated with papain (20 minutes). The volume, filancia, concentration of sperm, percentage of live sperm, percentage of functionality of the sperm membrane, and percentage of sperm with acrosomal reaction were evaluated. Sperm mobility and its kinetics were evaluated using a CASA-Mot system (ISAS®), determining the total motility (MT) and progressive motility (MP), as well as the sperm kinetic parameters: curvilinear velocity (VCL), straight-line velocity (VSL), average path velocity (VAP), and the indices of ...

The aim of this study was to determine the effect of papain treatment on the kinetics of llama sperm. Four 5-8-year-old Ch'aku male llamas were selected and semen was collected using an artificial vagina and a dummy. Four semen collections were made per animal with intervals of one week. The semen samples were separated into two aliquots. One for fresh analysis and the other to be treated with papain (20 minutes). The volume, filancia, concentration of sperm, percentage of live sperm, percentage of functionality of the sperm membrane, and percentage of sperm with acrosomal reaction were evaluated. Sperm mobility and its kinetics were evaluated using a CASA-Mot system (ISAS®), determining the total motility (MT) and progressive motility (MP), as well as the sperm kinetic parameters: curvilinear velocity (VCL), straight-line velocity (VSL), average path velocity (VAP), and the indices of ...