The aim of this study was to determine the existence of sperm subpopulations according to the morphometry of llama sperm, using a CASA-Morph system. Semen was collected from four 4-6-year-old Q'ara llamas by electroejaculation four times per animal, at one week intervals. The volume, total motility, sperm concentration, percentage of live sperm and percentage of sperm with acrosomal reaction were determined. The morphometric parameters of the sperm were evaluated using the CASA-Morph, Integrated Semen Analysis System (ISAS®v1). The length, width, area, perimeter, ellipticity, elongation, regularity and rugosity of the llama sperm head were determined, as well as the width, area, and distance and angle of insertion of the midpiece of the spermatozoa. The morphometric variables were distributed in five main components (PCA) called elongation, length, circularity, width of the midpiece and...

The aim of this study was to determine the existence of sperm subpopulations according to the morphometry of llama sperm, using a CASA-Morph system. Semen was collected from four 4-6-year-old Q'ara llamas by electroejaculation four times per animal, at one week intervals. The volume, total motility, sperm concentration, percentage of live sperm and percentage of sperm with acrosomal reaction were determined. The morphometric parameters of the sperm were evaluated using the CASA-Morph, Integrated Semen Analysis System (ISAS®v1). The length, width, area, perimeter, ellipticity, elongation, regularity and rugosity of the llama sperm head were determined, as well as the width, area, and distance and angle of insertion of the midpiece of the spermatozoa. The morphometric variables were distributed in five main components (PCA) called elongation, length, circularity, width of the midpiece and...

The aim of this study was to determine the effect of papain treatment on the kinetics of llama sperm. Four 5-8-year-old Ch'aku male llamas were selected and semen was collected using an artificial vagina and a dummy. Four semen collections were made per animal with intervals of one week. The semen samples were separated into two aliquots. One for fresh analysis and the other to be treated with papain (20 minutes). The volume, filancia, concentration of sperm, percentage of live sperm, percentage of functionality of the sperm membrane, and percentage of sperm with acrosomal reaction were evaluated. Sperm mobility and its kinetics were evaluated using a CASA-Mot system (ISAS®), determining the total motility (MT) and progressive motility (MP), as well as the sperm kinetic parameters: curvilinear velocity (VCL), straight-line velocity (VSL), average path velocity (VAP), and the indices of ...

The aim of this study was to determine the effect of papain treatment on the kinetics of llama sperm. Four 5-8-year-old Ch'aku male llamas were selected and semen was collected using an artificial vagina and a dummy. Four semen collections were made per animal with intervals of one week. The semen samples were separated into two aliquots. One for fresh analysis and the other to be treated with papain (20 minutes). The volume, filancia, concentration of sperm, percentage of live sperm, percentage of functionality of the sperm membrane, and percentage of sperm with acrosomal reaction were evaluated. Sperm mobility and its kinetics were evaluated using a CASA-Mot system (ISAS®), determining the total motility (MT) and progressive motility (MP), as well as the sperm kinetic parameters: curvilinear velocity (VCL), straight-line velocity (VSL), average path velocity (VAP), and the indices of ...