This study aimed to evaluate the effect of intramuscular application (IM) of seminal plasma on embryo survival in the alpaca after natural mating. The study was conducted in Puno, Peru. Seminal plasma was obtained from alpaca semen samples, which were collected and diluted 1:1 in phosphate buffered saline (PBS), then centrifuged and kept frozen in storage until use. Non-pregnant alpacas (n=117) were selected according to the presence of a dominant ovarian follicle (>7 mm) detected by transrectal ultrasonography. Females were naturally mated and assigned randomly into one of three groups: T1 (n=40): 1 ml of seminal plasma diluted in PBS, IM; T2 (n=39): 1 ml of GnRH analogue (0.0042 mg buserelin), IM; T3 (n=38) without administration of any product (control). Females were evaluated by transrectal ultrasonography on day 25 after mating to determine pregnancy and on day 62 for determining...

This study aimed to evaluate the effect of intramuscular application (IM) of seminal plasma on embryo survival in the alpaca after natural mating. The study was conducted in Puno, Peru. Seminal plasma was obtained from alpaca semen samples, which were collected and diluted 1:1 in phosphate buffered saline (PBS), then centrifuged and kept frozen in storage until use. Non-pregnant alpacas (n=117) were selected according to the presence of a dominant ovarian follicle (>7 mm) detected by transrectal ultrasonography. Females were naturally mated and assigned randomly into one of three groups: T1 (n=40): 1 ml of seminal plasma diluted in PBS, IM; T2 (n=39): 1 ml of GnRH analogue (0.0042 mg buserelin), IM; T3 (n=38) without administration of any product (control). Females were evaluated by transrectal ultrasonography on day 25 after mating to determine pregnancy and on day 62 for determining...

Se evaluó el efecto de la aplicación por vía intramuscular (IM) de plasma seminal sobre la supervivencia embrionaria en alpacas luego de la monta natural. El estudio se llevó a cabo en Puno, Perú. El plasma seminal se obtuvo de muestras de semen de alpaca. El semen fue colectado y diluido en proporción 1:1 en buffer fosfato salino (PBS), y posteriormente centrifugado y conservado en congelación. Se seleccionaron alpacas hembras vacías (n=117) con presencia de un folículo ovárico dominante (e»7 mm) detectado por ecografía transrectal. Los animales fueron servidos por monta natural y distribuidos aleatoriamente en tres grupos: T1(n=40), se les administró 1 ml de plasma seminal diluido en PBS, vía IM; T2(n=39), se les administró 1 ml de un análogo de GnRH (0.0042 mg de acetato de buserelina), vía IM; T3(n=38), sin administración alguna (control). Se hizo una evaluación me...

The aim if this study was to determine the effect of copulation time and the intramuscular application of seminal plasma (PS) in alpacas on pregnancy rate. A total of 161 female alpacas with ≥15 days postpartum, with calf at foot, and with the presence of preovulatory follicle ≥7 mm, identified by ultrasonography were used. The animals were randomized into six groups: G1 (n = 28) 5 min of copula without PS application; G2 (n = 28) 5 min of copula with application of 1 ml of PS i.m.; G3 (n = 27) 10 min of copula without application of PS; G4 (n = 27) 10 min of copula plus 1 ml of PS; G5 (n = 26) >15 min of copula without PS; G6 (n = 25) >15 min of copula with 1 ml PS. To avoid the male effect, the same animal was used to mate a female from the PS group and another from the control group for each study time. Pregnancy diagnosis was done by transrectal ultrasonography 25 days afte...

The aim if this study was to determine the effect of copulation time and the intramuscular application of seminal plasma (PS) in alpacas on pregnancy rate. A total of 161 female alpacas with ≥15 days postpartum, with calf at foot, and with the presence of preovulatory follicle ≥7 mm, identified by ultrasonography were used. The animals were randomized into six groups: G1 (n = 28) 5 min of copula without PS application; G2 (n = 28) 5 min of copula with application of 1 ml of PS i.m.; G3 (n = 27) 10 min of copula without application of PS; G4 (n = 27) 10 min of copula plus 1 ml of PS; G5 (n = 26) >15 min of copula without PS; G6 (n = 25) >15 min of copula with 1 ml PS. To avoid the male effect, the same animal was used to mate a female from the PS group and another from the control group for each study time. Pregnancy diagnosis was done by transrectal ultrasonography 25 days afte...

The effect of the stage of development of the follicular wave (growth phase, static and regression of the dominant follicle) prior to the natural mounting on the ovulation rate, and the recovery rate and embryo quality in alpacas was evaluated. It was used 51 female alpacas with presence of dominant follicle greater than or equal to 7 mm, which were synchronized with a GnRH analogue. The groups were T1 (n=16): growth phase; T2 (n=19): Static phase and T3 (n=18): Regression phase. The day of the copula was considered as day 0. Ultrasonographic evaluations were conducted on day 2 (occurrence of ovulation) and on day 7 (to measure the size of the corpus luteum). The embryo recovering was done 7 days after the service. Two animals of T1 did not ovulate. There were no significant differences between groups by size of the dominant follicle at the time of service, ovulation rate or size of the ...

The effect of the stage of development of the follicular wave (growth phase, static and regression of the dominant follicle) prior to the natural mounting on the ovulation rate, and the recovery rate and embryo quality in alpacas was evaluated. It was used 51 female alpacas with presence of dominant follicle greater than or equal to 7 mm, which were synchronized with a GnRH analogue. The groups were T1 (n=16): growth phase; T2 (n=19): Static phase and T3 (n=18): Regression phase. The day of the copula was considered as day 0. Ultrasonographic evaluations were conducted on day 2 (occurrence of ovulation) and on day 7 (to measure the size of the corpus luteum). The embryo recovering was done 7 days after the service. Two animals of T1 did not ovulate. There were no significant differences between groups by size of the dominant follicle at the time of service, ovulation rate or size of the ...