El objetivo del presente estudio fue evaluar la susceptibilidad del cuerpo lúteo al cloprostenol sódico (análogo sintético de la PGF2a) durante el desarrollo de la fase luteal en alpacas inducidas a ovulación con plasma seminal o con GnRH. El plasma seminal se obtuvo de muestras de semen de alpaca colectadas mediante vagina artificial, y diluido en proporción 1:1 en buffer fosfato salino (PBS), centrifugado y conservado a -20 °C. Se utilizaron 96 alpacas hembras con folículo >7 mm que fueron inducidas a ovulación mediante la inyección de plasma seminal (n=48) y GnRH (n=48). Las alpacas que ovularon fueron distribuidas en forma aleatoria en 6 grupos: un grupo control y 5 grupos tratados con PGF2a los días 4, 5, 6, 7 y 8 posinducción de ovulación. Se colectaron muestras de sangre para determinar los perfiles de progesterona sérica al inicio y 24 horas del tratamiento. No h...

El objetivo del presente estudio fue evaluar la susceptibilidad del cuerpo lúteo al cloprostenol sódico (análogo sintético de la PGF2a) durante el desarrollo de la fase luteal en alpacas inducidas a ovulación con plasma seminal o con GnRH. El plasma seminal se obtuvo de muestras de semen de alpaca colectadas mediante vagina artificial, y diluido en proporción 1:1 en buffer fosfato salino (PBS), centrifugado y conservado a -20 °C. Se utilizaron 96 alpacas hembras con folículo >7 mm que fueron inducidas a ovulación mediante la inyección de plasma seminal (n=48) y GnRH (n=48). Las alpacas que ovularon fueron distribuidas en forma aleatoria en 6 grupos: un grupo control y 5 grupos tratados con PGF2a los días 4, 5, 6, 7 y 8 posinducción de ovulación. Se colectaron muestras de sangre para determinar los perfiles de progesterona sérica al inicio y 24 horas del tratamiento. No h...

The effect of the stage of development of the follicular wave (growth phase, static and regression of the dominant follicle) prior to the natural mounting on the ovulation rate, and the recovery rate and embryo quality in alpacas was evaluated. It was used 51 female alpacas with presence of dominant follicle greater than or equal to 7 mm, which were synchronized with a GnRH analogue. The groups were T1 (n=16): growth phase; T2 (n=19): Static phase and T3 (n=18): Regression phase. The day of the copula was considered as day 0. Ultrasonographic evaluations were conducted on day 2 (occurrence of ovulation) and on day 7 (to measure the size of the corpus luteum). The embryo recovering was done 7 days after the service. Two animals of T1 did not ovulate. There were no significant differences between groups by size of the dominant follicle at the time of service, ovulation rate or size of the ...

The effect of the stage of development of the follicular wave (growth phase, static and regression of the dominant follicle) prior to the natural mounting on the ovulation rate, and the recovery rate and embryo quality in alpacas was evaluated. It was used 51 female alpacas with presence of dominant follicle greater than or equal to 7 mm, which were synchronized with a GnRH analogue. The groups were T1 (n=16): growth phase; T2 (n=19): Static phase and T3 (n=18): Regression phase. The day of the copula was considered as day 0. Ultrasonographic evaluations were conducted on day 2 (occurrence of ovulation) and on day 7 (to measure the size of the corpus luteum). The embryo recovering was done 7 days after the service. Two animals of T1 did not ovulate. There were no significant differences between groups by size of the dominant follicle at the time of service, ovulation rate or size of the ...