El gen Lvp53 del langostino Litopenaeus vannamei (Lvp53) ha sido caracterizado a nivel del ADN complementario (ADNc), encontrando motivos homólogos a p53 de humanos y relación con la respuesta a diferentes tipos de estrés. Sin embargo, aún se desconoce la estructura de este gen, así como su funcionalidad. En este estudio se propuso caracterizar parcialmente la secuencia genómica del gen Lvp53, estudiar su expresión ante un estrés térmico e implementar metodologías de transfección in vivo y mutagénesis dirigida mediada por TALENs para determinar la relación entre la pérdida de la actividad de Lvp53 con el ciclo celular. Se identificaron dos regiones intrónicas putativas con posibles sitios funcionales de Lvp53. Se observó regulación positiva de la expresión del transcrito ante variaciones de temperatura alcanzando mayores niveles cuando ésta es incrementada. Además, se...

The objective of this study was to evaluate the in vitro embryo production rate up to the blastocyst stage with two fertilization media: FERT-TALP and modified TCM-199, grown in BOEC medium, from oocytes collected by aspiration of follicular ovaries of creole cows obtained from the slaughterhouse. In total, 281 oocytes were collected, 179 for FERT-TALP medium and 144 for modified TCM-199. The oocytes were subjected to the process of maturation, fertilization and embryo culture in vitro. An oocyte cleavage rate of 25.8% was obtained in FERT-TALP and 2.4% in modified TCM-199 at 18 hours post-fertilization. Likewise, at 48h, 87.7% of total cleavage was obtained in FER-TALP and 40.5% in modified TCM-199, whose percentage of embryos with 4 to 8 cells was 64.5 and 26.2%, respectively. In the morula and blastocyst phase, a rate of 10.3 and 3.9% was achieved in FERT-TALP, respectively, while non...

The effect of six cryoprotective solutions based on MgCl2 and methanol, in addtion with sucrose and 10% Aloe vera gel or 10% egg yolk, on the sperm viability in spermatophore and sperm mass of Litopenaeus vannamei was evaluated. Besides, cooling rates of -0.5, -1 and -2 °C/min were used until temperature reached -35, -80 and -100 °C prior to immersion in liquid nitrogen. The sperm viability rate was determined by eosin-nigrosin staining. The results showed that the solutions based on MgCl2, sucrose plus egg yolk or Aloe vera gel better preserved sperm viability in spermatophore (82.8 ± 2.3% and 72.6 ± 3.1%) and in sperm mass (83.4 ± 2.5% and 76 ± 1.1%) without significant differences with the control group (p>0.05). The freezing curve showing less damage in spermatophore started from 25 °C and reached -35 °C with a rate of -1 °C/min using a cryoprotec...

The effect of six cryoprotective solutions based on MgCl2 and methanol, in addtion with sucrose and 10% Aloe vera gel or 10% egg yolk, on the sperm viability in spermatophore and sperm mass of Litopenaeus vannamei was evaluated. Besides, cooling rates of -0.5, -1 and -2 °C/min were used until temperature reached -35, -80 and -100 °C prior to immersion in liquid nitrogen. The sperm viability rate was determined by eosin-nigrosin staining. The results showed that the solutions based on MgCl2, sucrose plus egg yolk or Aloe vera gel better preserved sperm viability in spermatophore (82.8 ± 2.3% and 72.6 ± 3.1%) and in sperm mass (83.4 ± 2.5% and 76 ± 1.1%) without significant differences with the control group (p>0.05). The freezing curve showing less damage in spermatophore started from 25 °C and reached -35 °C with a rate of -1 °C/min using a cryoprotec...