Effect of two fertilization medium on the in vitro development of Creole bovine embryosne embryos

• Autores: Goicochea Vargas, José, Rondón Jorge, Wilson, Acosta Pachorro, Fidel, Gómez Marín, Yusep, Montalvo Martin, Magaly, Salvatierra Alor, Max, Martel Falcón, Jéssica, Ballarte Zevallos, Oscar, Díaz Zegarra, Julio, Ratto Fuster, Marcelo
• Formato: artículo
• Fecha de Publicación: 2021
• Institución: Universidad Nacional Mayor de San Marcos
• Repositorio: Revistas - Universidad Nacional Mayor de San Marcos
• Lenguaje: español
• OAI Identifier: oai:ojs.csi.unmsm:article/18709
• Enlace del recurso: https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/18709
• Nivel de acceso: acceso abierto
• Materia: FERT-TALP; TCM-199; in vitro; embryos; native cattle; embriones; bovinos criollos

The objective of this study was to evaluate the in vitro embryo production rate up to the blastocyst stage with two fertilization media: FERT-TALP and modified TCM-199, grown in BOEC medium, from oocytes collected by aspiration of follicular ovaries of creole cows obtained from the slaughterhouse. In total, 281 oocytes were collected, 179 for FERT-TALP medium and 144 for modified TCM-199. The oocytes were subjected to the process of maturation, fertilization and embryo culture in vitro. An oocyte cleavage rate of 25.8% was obtained in FERT-TALP and 2.4% in modified TCM-199 at 18 hours post-fertilization. Likewise, at 48h, 87.7% of total cleavage was obtained in FER-TALP and 40.5% in modified TCM-199, whose percentage of embryos with 4 to 8 cells was 64.5 and 26.2%, respectively. In the morula and blastocyst phase, a rate of 10.3 and 3.9% was achieved in FERT-TALP, respectively, while none wwere achieved with the modified TCM-199 medium.