En la presente investigación se estudia la situación epidemiológica de la Peste porcina clásica (PPC) en el Perú durante el periodo 2015 – 2019, entendiéndose por situación epidemiológica a la presentación de casos positivos, brotes, focos, gastos por el análisis de las muestras remitidas y pérdidas económicas por sacrificio, con el objetivo de determinar estos indicadores, la curva epidemiológica de la enfermedad y predisposición de la PPC a factores como el sexo, edad y el tipo de explotación pecuaria. En este periodo se presentó 16.3% de casos positivos, 49.8% en el 2015, 42.6% en el 2016, ningún caso en el 2017, 0.1% en el 2018 y 9.8% en el 2019. Los brotes encontrados fueron: En marzo del 2015 en Ucayali; abril del mismo año en Amazonas y julio del 2016 en La Libertad; en relación a los focos: enero 2015 en el departamento de Moquegua, marzo de ese mismo año en...

The clinical effects, sperm parameters and macroscopic and histological lesions caused by chemical castration in dogs through the application of sclerosing substances were evaluated. Three groups were established: (G1, n=10), 4% chlorhexidine gluconate, (G2, n=10), 4% benzalkonium chloride + 0.4% cetrimide, and (G3, control, n=5) chloride of 0.9% sodium, at a dose of 0.5-1.0 ml intra-epididymal (IEPD) and bilateral on day 0. The effects were evaluated on days 2, 7, 14, 28 and 56 post-application. The manifest oligospermia was observed in 9/10 animals of G1 (90%) and in 7/10 of G2 at day 2 post-application, with a critical decrease of sperm concentration (3.78 ± 6.3 x 106/ml and 0.95 ± 0.89 x 106/ml, respectively, motility of 0.10 ± 0.32 - 0.20 ± 0.42% with oscillatory movement, without progressive advance and vitality of 0.30 ± 0.67% - 0% in both groups. Azoospermia was detected in ...

The clinical effects, sperm parameters and macroscopic and histological lesions caused by chemical castration in dogs through the application of sclerosing substances were evaluated. Three groups were established: (G1, n=10), 4% chlorhexidine gluconate, (G2, n=10), 4% benzalkonium chloride + 0.4% cetrimide, and (G3, control, n=5) chloride of 0.9% sodium, at a dose of 0.5-1.0 ml intra-epididymal (IEPD) and bilateral on day 0. The effects were evaluated on days 2, 7, 14, 28 and 56 post-application. The manifest oligospermia was observed in 9/10 animals of G1 (90%) and in 7/10 of G2 at day 2 post-application, with a critical decrease of sperm concentration (3.78 ± 6.3 x 106/ml and 0.95 ± 0.89 x 106/ml, respectively, motility of 0.10 ± 0.32 - 0.20 ± 0.42% with oscillatory movement, without progressive advance and vitality of 0.30 ± 0.67% - 0% in both groups. Azoospermia was detected in ...

The aim of this study was to evaluate the effect of GnRH, hCG and eCG applied 14 days after fixed time artificial insemination (FTAI) on the rate of pregnancy and embryonic and foetal survival in cows under heat stress. The cows (n = 234) were synchronized, inseminated and received 14 days later via i.m. the hormonal application: a) Control group: 2 ml of saline solution (n=56), b) GnRH group: 100 µg of GnRH (n=58), Group c) hCG: 1500 IU of hCG (n=60), and d) ECG Group: 400 IU of eCG (n=60). The diagnosis of pregnancy and the evaluation of the number of corpora lutea (CL) was determined by ultrasonography on days 34, 45 and 60 of the AI. Cows treated with GnRH had a higher pregnancy rate (p<0.05) and no significant differences were found between the other groups. Embryonic and foetal survival between days 45 and 60 was constant in all groups, but one foetal death occurred in the eCG ...

The aim of this study was to evaluate the effect of GnRH, hCG and eCG applied 14 days after fixed time artificial insemination (FTAI) on the rate of pregnancy and embryonic and foetal survival in cows under heat stress. The cows (n = 234) were synchronized, inseminated and received 14 days later via i.m. the hormonal application: a) Control group: 2 ml of saline solution (n=56), b) GnRH group: 100 µg of GnRH (n=58), Group c) hCG: 1500 IU of hCG (n=60), and d) ECG Group: 400 IU of eCG (n=60). The diagnosis of pregnancy and the evaluation of the number of corpora lutea (CL) was determined by ultrasonography on days 34, 45 and 60 of the AI. Cows treated with GnRH had a higher pregnancy rate (p<0.05) and no significant differences were found between the other groups. Embryonic and foetal survival between days 45 and 60 was constant in all groups, but one foetal death occurred in the eCG ...

The objective of this study was to evaluate the in vitro embryo production rate up to the blastocyst stage with two fertilization media: FERT-TALP and modified TCM-199, grown in BOEC medium, from oocytes collected by aspiration of follicular ovaries of creole cows obtained from the slaughterhouse. In total, 281 oocytes were collected, 179 for FERT-TALP medium and 144 for modified TCM-199. The oocytes were subjected to the process of maturation, fertilization and embryo culture in vitro. An oocyte cleavage rate of 25.8% was obtained in FERT-TALP and 2.4% in modified TCM-199 at 18 hours post-fertilization. Likewise, at 48h, 87.7% of total cleavage was obtained in FER-TALP and 40.5% in modified TCM-199, whose percentage of embryos with 4 to 8 cells was 64.5 and 26.2%, respectively. In the morula and blastocyst phase, a rate of 10.3 and 3.9% was achieved in FERT-TALP, respectively, while non...