El siguiente trabajo se realizó en el centro de producción de Kotosh y en la Facultad de Medicina Veterinaria de la UNHEVAL. El objetivo de este estudio fue comparar la morfometría histológica de la pared uterina de la alpaca en diestro (día 8), post ovulación con GNRH y cópula. Se evaluaron dos grupos experimentales G1 (GNRH, n=3) y G2 (Cópula, n=5), las alpacas fueron ecografiadas, ante la presencia de un folículo ≥ de 7 mm. se indujo la ovulación en ambos grupos, posteriormente se ecografio 24 horas para confirmar la desaparición del folículo y formación del cuerpo lúteo. El día 8 post ovulación, se sometieron a una laparotomía mediana para extraer una muestra de pared uterina derecha e izquierda. Los tejidos fueron fijados y procesados, para luego ser teñidas en Hematoxilina y Eosina, después fueron analizados y medidos en micras por un microscopio. Se observó q...

El presente estudio se realizó en la Facultad de medicina veterinaria de la Universidad Nacional Hermilio Valdizán de Huánuco, con la finalidad de detallar las caracterizas histológica de la pared uterina del día 4 y 8 de la fase lútea inducida. Siendo característico apreciar el día 4 de la fase lútea, glándulas endometriales de ambos cuernos uterinos la presencia de un tejido cilíndrico simple con núcleos con fondos glandulares sin presencia de contenido y orientadas hacia el miometrio. El día 8, se evidencia un ligero aumento de la densidad glandular funcional con células altas y núcleos basales y orientadas hacia la mucosa, las glándulas endometriales son tubulares con fluido secretor formadas por células altas y núcleos basales, siendo más evidente en aquellas que se encuentran más próximas al lumen. Las características más notorias entre el cuerno derecho e iz...

The clinical effects, sperm parameters and macroscopic and histological lesions caused by chemical castration in dogs through the application of sclerosing substances were evaluated. Three groups were established: (G1, n=10), 4% chlorhexidine gluconate, (G2, n=10), 4% benzalkonium chloride + 0.4% cetrimide, and (G3, control, n=5) chloride of 0.9% sodium, at a dose of 0.5-1.0 ml intra-epididymal (IEPD) and bilateral on day 0. The effects were evaluated on days 2, 7, 14, 28 and 56 post-application. The manifest oligospermia was observed in 9/10 animals of G1 (90%) and in 7/10 of G2 at day 2 post-application, with a critical decrease of sperm concentration (3.78 ± 6.3 x 106/ml and 0.95 ± 0.89 x 106/ml, respectively, motility of 0.10 ± 0.32 - 0.20 ± 0.42% with oscillatory movement, without progressive advance and vitality of 0.30 ± 0.67% - 0% in both groups. Azoospermia was detected in ...

The clinical effects, sperm parameters and macroscopic and histological lesions caused by chemical castration in dogs through the application of sclerosing substances were evaluated. Three groups were established: (G1, n=10), 4% chlorhexidine gluconate, (G2, n=10), 4% benzalkonium chloride + 0.4% cetrimide, and (G3, control, n=5) chloride of 0.9% sodium, at a dose of 0.5-1.0 ml intra-epididymal (IEPD) and bilateral on day 0. The effects were evaluated on days 2, 7, 14, 28 and 56 post-application. The manifest oligospermia was observed in 9/10 animals of G1 (90%) and in 7/10 of G2 at day 2 post-application, with a critical decrease of sperm concentration (3.78 ± 6.3 x 106/ml and 0.95 ± 0.89 x 106/ml, respectively, motility of 0.10 ± 0.32 - 0.20 ± 0.42% with oscillatory movement, without progressive advance and vitality of 0.30 ± 0.67% - 0% in both groups. Azoospermia was detected in ...

The aim of this study was to evaluate the effect of GnRH, hCG and eCG applied 14 days after fixed time artificial insemination (FTAI) on the rate of pregnancy and embryonic and foetal survival in cows under heat stress. The cows (n = 234) were synchronized, inseminated and received 14 days later via i.m. the hormonal application: a) Control group: 2 ml of saline solution (n=56), b) GnRH group: 100 µg of GnRH (n=58), Group c) hCG: 1500 IU of hCG (n=60), and d) ECG Group: 400 IU of eCG (n=60). The diagnosis of pregnancy and the evaluation of the number of corpora lutea (CL) was determined by ultrasonography on days 34, 45 and 60 of the AI. Cows treated with GnRH had a higher pregnancy rate (p<0.05) and no significant differences were found between the other groups. Embryonic and foetal survival between days 45 and 60 was constant in all groups, but one foetal death occurred in the eCG ...

The aim of this study was to evaluate the effect of GnRH, hCG and eCG applied 14 days after fixed time artificial insemination (FTAI) on the rate of pregnancy and embryonic and foetal survival in cows under heat stress. The cows (n = 234) were synchronized, inseminated and received 14 days later via i.m. the hormonal application: a) Control group: 2 ml of saline solution (n=56), b) GnRH group: 100 µg of GnRH (n=58), Group c) hCG: 1500 IU of hCG (n=60), and d) ECG Group: 400 IU of eCG (n=60). The diagnosis of pregnancy and the evaluation of the number of corpora lutea (CL) was determined by ultrasonography on days 34, 45 and 60 of the AI. Cows treated with GnRH had a higher pregnancy rate (p<0.05) and no significant differences were found between the other groups. Embryonic and foetal survival between days 45 and 60 was constant in all groups, but one foetal death occurred in the eCG ...

The objective of this study was to evaluate the in vitro embryo production rate up to the blastocyst stage with two fertilization media: FERT-TALP and modified TCM-199, grown in BOEC medium, from oocytes collected by aspiration of follicular ovaries of creole cows obtained from the slaughterhouse. In total, 281 oocytes were collected, 179 for FERT-TALP medium and 144 for modified TCM-199. The oocytes were subjected to the process of maturation, fertilization and embryo culture in vitro. An oocyte cleavage rate of 25.8% was obtained in FERT-TALP and 2.4% in modified TCM-199 at 18 hours post-fertilization. Likewise, at 48h, 87.7% of total cleavage was obtained in FER-TALP and 40.5% in modified TCM-199, whose percentage of embryos with 4 to 8 cells was 64.5 and 26.2%, respectively. In the morula and blastocyst phase, a rate of 10.3 and 3.9% was achieved in FERT-TALP, respectively, while non...