Tomato (Solanum lycopersicum) (Solanaceae) is an important vegetable crop worldwide that contains significant amounts of vitamins A and C. It also possesses a powerful antioxidant, lycopene, which can help prevent the development of many forms of cancer. However, this vegetable is highly susceptible to a number of emerging viruses. Since the first report of ToBRFV in Jordan, this emerging virus has been detected in Germany, Israel, Italy, Mexico, Palestine, and the United States, but its incidence was not reported in Peru. We collected 56 samples of fresh leaves of tomato plants with viral symptoms and 13 without symptoms as control from two regions that comprise more than 50% of tomato production in Peru, Lima and Ica. Mosaic, mottling, plant stunting and brown rugose symptoms were observed in collected leaves that were preserved in liquid nitrogen until processing. We extracted RNA usi...

Objetivo: Identificar molecularmente los virus ARN que afectan a cultivares de tomate en la costa del Perú. Materiales y métodos: Se recogieron hojas de plantaciones de tomate que presentaron sintomatología por infección viral, las hojas colectadas se les realizó una molienda con nitrógeno líquido para lograr extraer el ARN utilizando un kit comercial y se le realizó una digestión con la enzima DNAsa para tener una muestra pura de ARN sin rasgos de ADN. Se realizó para sintetizar el ADN complementario, luego de se empleó una PCR, determinando sus condiciones óptimas de amplificación, los resultados se observaron en una electroforesis en gel de agarosa, las muestras que fueron positivas para los patógenos por PCR, se enviaron a secuenciar al extranjero. Las muestras de los virus secuenciados se alinearon con otras secuencias de virus reportados, que se descargaron del GenBan...

16 páginas

Lupinus mutabilis Sweet (Fabaceae), “tarwi” or “chocho”, is an important grain legume in the Andean region. In Peru, studies on tarwi have mainly focused on morphological features; however, they have not been molecularly characterized. Currently, it is possible to explore the genetic parameters of plants with reliable and modern methods such as genotyping by sequencing (GBS). Here, for the first time, we used single nucleotide polymorphism (SNP) markers to infer the genetic diversity and population structure of 89 accessions of tarwi from nine Andean regions of Peru. A total of 5922 SNPs distributed along all chromosomes of tarwi were identified. STRUCTURE analysis revealed that this crop is grouped into two clusters. A dendrogram was generated using the UPGMA clustering algorithm and, like the principal coordinate analysis (PCoA), it showed two groups that correspond to the geog...

Lupinus mutabilis Sweet (Fabaceae), “tarwi” or “chocho”, is an important grain legume in the Andean region. In Peru, studies on tarwi have mainly focused on morphological features; however, they have not been molecularly characterized. Currently, it is possible to explore the genetic parameters of plants with reliable and modern methods such as genotyping by sequencing (GBS). Here, for the first time, we used single nucleotide polymorphism (SNP) markers to infer the genetic diversity and population structure of 89 accessions of tarwi from nine Andean regions of Peru. A total of 5922 SNPs distributed along all chromosomes of tarwi were identified. STRUCTURE analysis revealed that this crop is grouped into two clusters. A dendrogram was generated using the UPGMA clustering algorithm and, like the principal coordinate analysis (PCoA), it showed two groups that correspond to the geog...

Capirona (Calycophyllum spruceanum Benth.) belongs to subfamily Ixoroideae, one of the major lineages in the Rubiaceae family, and is an important timber tree. It originated in the Amazon Basin and has widespread distribution in Bolivia, Peru, Colombia, and Brazil. In this study, we obtained the first complete chloroplast (cp) genome of capirona from the department of Madre de Dios located in the Peruvian Amazon. High-quality genomic DNA was used to construct libraries. Pair-end clean reads were obtained by PE 150 library and the Illumina HiSeq 2500 platform. The complete cp genome of C. spruceanum has a 154,480 bp in length with typical quadripartite structure, containing a large single copy (LSC) region (84,813 bp) and a small single-copy (SSC) region (18,101 bp), separated by two inverted repeat (IR) regions (25,783 bp). The annotation of C. spruceanum cp genome predicted 87 protein-c...