The purpose of this study was to determine the in vitro antileishmanial activity of the methanol extract of seven medicinal plants, and the effect on nitric oxide production in macrophages. The extracts in concentration of 250 μg/mL were tested against intracellular amastigotes. Mouse peritoneal macrophages infected with Leishmania peruviana were stained with Giemsa and examined microscopically to determine the parasite load by 100 macrophages. Nitric oxide was determined indirectly by its conversion to nitrite. Piper aduncum (matico) reduced the survival of amastigotes to 51,30% (p < 0,001), also observed a slight effect of Eucalyptus globulus (eucalyptus) and Punica granatum (pomegranate), while Annona cherimola (custard), Annona muricata (graviola) and Origanum vulgare (oregano) were inactive. Nitric oxide production by macrophages was increased significantly by the effect of Anno...

The purpose of this study was to determine the in vitro antileishmanial activity of the methanol extract of seven medicinal plants, and the effect on nitric oxide production in macrophages. The extracts in concentration of 250 μg/mL were tested against intracellular amastigotes. Mouse peritoneal macrophages infected with Leishmania peruviana were stained with Giemsa and examined microscopically to determine the parasite load by 100 macrophages. Nitric oxide was determined indirectly by its conversion to nitrite. Piper aduncum (matico) reduced the survival of amastigotes to 51,30% (p < 0,001), also observed a slight effect of Eucalyptus globulus (eucalyptus) and Punica granatum (pomegranate), while Annona cherimola (custard), Annona muricata (graviola) and Origanum vulgare (oregano) were inactive. Nitric oxide production by macrophages was increased significantly by the effect of Anno...

Objective: To determine anti-Trypanosoma cruzi effect of Cymbopogon citratus essential oil in Balb/c mice. Design: Experimental, prospective study. Setting: Institute of Clinical Research and Institute of Tropical Medicine, Faculty of Medicine, Universidad Nacional Mayor de San Marcos, Lima, Peru. Biological material: Cymbopogon citrates essential oil; Balb/c albino mice. Interventions: Animals were randomly assigned to six groups (n = 15): infected and untreated (G1), infected and treated with benznidazole 100 mg/kg (G2), infected and treated with essential oil of Cymbopogon citratus 100 mg/kg and 250 mg/kg (G3 and G4, respectively), uninfected and untreated (G5), and uninfected and treated with 250 mg/kg of Cymbopogon citratus (G6). Infection with T. cruzi was conducted with 104 blood trypomastigotes and treatment began on 8th day post infection (dpi) through 28th dpi. Parasitemia was ...

Objetives: To determine the antihypertensive effect and lethal dose (LD) 50 of the fruit juice and ethanolic extract from Passiflora edulis leaves in rats. Design: Prospective, analytic-experimental, preclinical in vivo study. Setting: Faculties of Medicine, Chemistry and Chemical Engineering and Pharmacy and Biochemistry, San Marcos National University. Biologic material: Rats. Interventions: Four groups of rats (n = 6) were used to determine the antihypertensive effect. After basal determination, arterial hypertension was induced using L-NAME 50 mg/kd/d VO and two days later the treatment was initiated with distilled water, ethanolic extract (500 mg/kg/d) VO, fruit juice 500 mg/kg/d VO, captopril 100 mg/kg/d VO, respectively. Arterial pressure was measured on days 3, 4, 7 and 10. For determining LD50, 12 groups of mice (n=10) were used with 7 different dose levels of ethanolic extract,...

Objectives: To determine the adjunctive antihypertensive effect and safety of passion fruit juice in patients with hypertension treated with enalapril. Design: prospective pilot clinical trial, phase II, randomized, double blind, parallel group, controlled, dose finding and evaluation of the product. Setiing: Instituto de Investigaciones Clínicas, Facultad de Medicina, UNMSM, Dos de Mayo Hospital in Lima, Hospital Belen de Trujillo and Moche and Laredo Health Centers in Trujillo city. Participants: Patients with arterial hypertension. Interventions: Patients were randomly assigned to four groups, each receiving enalapril 10 mg / day; the first group also received placebo and the other, 2, 3 and 4 groups, 500 mg capsules of freeze-dried passion fruit juice / day, respectively. Main outcome measures: Reduction in blood pressure. Results: Groups receiving enalapril and passion fruit had be...

Fourteen year-old male patient referred from Huancayo who presented one month gastric intolerance, jaundice, fever and a lymph proliferative syndrome. Laboratory tests revealed severe pancytopenia due to phagocytosis. Haematologic and anatomy-pathology diagnosis was human malignant histiocytosis. We present this case due to its low frequency and the emergency character of the disease.

Background: In Peru, there is an increase in the creation of mobile health (mHealth) apps; however, this situation could present problems related to the quality of information these apps share, data security and privacy, usability, and effectiveness, as there is no specific local regulation about their creation and use. Objective: The objective of this study was to review mHealth apps created, uploaded, or used in Peru, and perform an analysis of the national regulatory framework that could be applied to evaluate whether there is a need to develop and implement a specific regulation to these apps. Methods: A total of 3 reviews were performed. First, we reviewed information about Peruvian mHealth apps created up to May 2019 from scientific publications, news, government communications, and virtual stores, and evaluated their purpose, creator, and the available evidence of their usability ...