The polyethylene biodegradation by microorganisms is a solution for the reduction of the plastics pollution. Isolation and characterization of the fungi capable to degrade the polyethylene are reported, as well as the conditions of pH and temperature in which they showed the higher activity. Fungi were isolated from polyethylene products obtained of sanitary landfill; the identification was realized in base to growth in petri plate and the technique of microculture in sheet. Biodegradative activity was determined with the Kavelman and Kendrick technique, for temperatures between 20 and 30 °C and to pH 4,5 – 8,0. Twenty strains of micromicete were isolated and identified. Five strain (25%) shown polyethylene biodegradative capacity to 20 °C, and pH 6,5. Six strain (30%) showed activity biodegradative to 30 °C, and pH 6,5. In both cases, Aspergillus flavus was the strain with greater ...

The aim of the study was to isolate and characterize E. coli O157: H7 from fresh ground beef obtained in different food markets. 195 samples were analyzed, for isolation and enumeration of E. coliwas used most probable number technique using multiple tubes, for the isolation and characterization of E. coliO157: H7 selective enrichment and biochemical analysis, characteristic colonies were confirmed serologically. To determine the presence of shigatoxin (stx1, stx2) and intimin (eaeA) was done with multiplex real time PCR and for enterohemolysin the hemolysis test. The 87.18% of the samples were positive for E. coliand 77.95% had a count greater than or equal to 50 NMP/g. Were obtained 3 (1.54%) strains of E. coliO157: H7, one stx1 +/stx2 +/eaeA - and enterohemolysin -, one stx1 +/stx2 -/eaeA + and enterohemolysin - and the other stx1 -/stx2 -/eaeA - and enterohemolysin +. Also obtained 4...

The polyethylene biodegradation by microorganisms is a solution for the reduction of the plastics pollution. Isolation and characterization of the fungi capable to degrade the polyethylene are reported, as well as the conditions of pH and temperature in which they showed the higher activity. Fungi were isolated from polyethylene products obtained of sanitary landfill; the identification was realized in base to growth in petri plate and the technique of microculture in sheet. Biodegradative activity was determined with the Kavelman and Kendrick technique, for temperatures between 20 and 30 °C and to pH 4,5 – 8,0. Twenty strains of micromicete were isolated and identified. Five strain (25%) shown polyethylene biodegradative capacity to 20 °C, and pH 6,5. Six strain (30%) showed activity biodegradative to 30 °C, and pH 6,5. In both cases, Aspergillus flavus was the strain with greater ...

The aim of the study was to isolate and characterize E. coli O157: H7 from fresh ground beef obtained in different food markets. 195 samples were analyzed, for isolation and enumeration of E. coliwas used most probable number technique using multiple tubes, for the isolation and characterization of E. coliO157: H7 selective enrichment and biochemical analysis, characteristic colonies were confirmed serologically. To determine the presence of shigatoxin (stx1, stx2) and intimin (eaeA) was done with multiplex real time PCR and for enterohemolysin the hemolysis test. The 87.18% of the samples were positive for E. coliand 77.95% had a count greater than or equal to 50 NMP/g. Were obtained 3 (1.54%) strains of E. coliO157: H7, one stx1 +/stx2 +/eaeA - and enterohemolysin -, one stx1 +/stx2 -/eaeA + and enterohemolysin - and the other stx1 -/stx2 -/eaeA - and enterohemolysin +. Also obtained 4...

La presente investigación tuvo como objetivo determinar la influencia de la plataforma Kahoot en la mejora del aprendizaje del idioma inglés en alumnos del cuarto grado de primaria de un colegio particular mixto de la ciudad de Ica. Para llevar a cabo esta investigación de tipo cuasi experimental se tomó como muestra a la totalidad de los alumnos del cuarto grado de primaria, consistente en 50 alumnos entre niños y niñas, a quienes se dividió en dos grupos de 25 alumnos cada uno, denominados grupo control y grupo experimental respectivamente, a quienes luego de la explicación del programa se sometió a dos evaluaciones a cada grupo, consistente en un pre test y un post test, relacionados al aprendizaje de la gramática y del vocabulario del idioma inglés. Luego de efectuar el análisis estadístico a ambos grupos mediante las pruebas de Wilcoxon y la U de Mann Whitney se determi...

El presente plan de negocios, nace de la necesidad de los padres de familia por un ambiente confiable, seguro e integral para sus niños. Nace como una respuesta a la tendencia de que ambos padres trabajan y no cuentan con el personal idóneo para el cuidado de estos, durante el horario laboral. En virtud a ello Lider Kids, será constituida bajo la Empresa Individual de Responsabilidad Limitada, cuya actividad principal será el servicio educativo para niños de las edades de 2 meses a 5 años, en el horario de 8:00 a.m. a 7:00 p.m. El sector de la enseñanza, ha registrado en los últimos años, tasas de crecimientos alentadoras, explicado por la buena coyuntura económica del país. Este crecimiento económico ha generado que más familias encuentren más empleo, y a la par mejoren sus ingresos familiares. El servicio educativo de “Lider Kids”, se orientará principalmente a los n...

Los Niños y Niñas de 05 años de la I.E.I. N°86675 del Caserío de Mitua, del distrito de Quillo, provincia de Yungay, han demostrado algunas dificultades en lo que respecta al área de matemática, cuando realizan actividades de agrupar, de correspondencia, al seriar, al hacer uso de cuantificadores y por último al realizar el conteo. Tal es la razón que nos motivó plantear un programa de actividades lúdicas como estrategia metodológica en las programaciones de actividades, organizada, dosificada y secuenciada, para desarrollar las nociones de cantidad. El presente trabajo académico denominado: “Programa de actividades lúdicas como estrategia para desarrollar nociones de cantidad en los niños y niñas de 05 años de la I.E.I. N°86675 del caserío de Mitua, fue aplicado a un grupo de 15 alumnos con el objetivo de demostrar que, con la aplicación de dichas actividades como ...

acceptable, then detect the pollution source. It was performed a microbiologicalanalysis of the water of 21 Lima’s beaches and the detections of contamination sources with human feces. The results qualify the water of 33.33% (07) beaches as unacceptable if it is used as indicators of fecal pollution, the total coliforms and fecal coliforms; if it is included Escherichia coli, it reaches to 47.62% (10); and if also it is included Enterococcus it reaches to 66.66%(14). It was demonstrated the existence of domestic sewage discharge in all the beaches considered as unacceptable; in 35.75% (05) with visual inspection; in 14.29% (02) was observed water running with fecal pollution and in 50% (07) by the relation between E. coli and Enterococcus.

In July (winter) of 2005 and January (summer) of 2006 there was realized the microbiological study of the water of 8 beaches of summery recreation of the coast of Lima by the purpose of evaluating the usefulness of Escherichia coli and Enterococcus like indicators of fecal pollution. In every month 5 series of samplings were done. They used like indicators of fecal pollution total Coliforms (CT) 5,000 NMP/100mL, fecal Coliforms (CF) 1,000 NMP/100mL according to the Peruvian Norm and also to Escherichia coli (EC) 100 NMP/100mL and Enterococcus (E) 35 NMP/100mL. The results of the analyses realized in January qualify to the water of 3 beaches like unacceptable for the bath, according to the Peruvian Norm; but it is used as indicator EC and E, 8 beaches would be considered to be like unacceptable. The results of July demonstrate that the water of 8 beaches was acceptable, according to the P...

To detect injured coliforms (IC) in drinking water considered suitable for the human consumption according to the Peruvian normativity, were taken and processed 100 water samples of buildings of the district of Lima Cercado. For the analysis the residual free chlorine (RFC) by means of DPD technique and pH was determined using indicating rods Merck. For the count of heterotrophic bacteria (HB) the spread plate method was used, to quantify total Coliform (TC) and fecal Coliform (FC) the techniques of multiple tubes and membrane fi lter were used. In order to recover and to enumerate injured TC and FC the m-T7 medium were used. For the bacterial specifi cation the standard tests and API 20E system. For CI’s detection there were in used the samples that turned out to be negative for TC and FC and/or had count of HB an equal or lower to 500 UFC/mL. Using the conventional methods was demons...

To detect injured coliforms (IC) in drinking water considered suitable for the human consumption according to the Peruvian normativity, were taken and processed 100 water samples of buildings of the district of Lima Cercado. For the analysis the residual free chlorine (RFC) by means of DPD technique and pH was determined using indicating rods Merck. For the count of heterotrophic bacteria (HB) the spread plate method was used, to quantify total Coliform (TC) and fecal Coliform (FC) the techniques of multiple tubes and membrane fi lter were used. In order to recover and to enumerate injured TC and FC the m-T7 medium were used. For the bacterial specifi cation the standard tests and API 20E system. For CI’s detection there were in used the samples that turned out to be negative for TC and FC and/or had count of HB an equal or lower to 500 UFC/mL. Using the conventional methods was demons...

In July (winter) of 2005 and January (summer) of 2006 there was realized the microbiological study of the water of 8 beaches of summery recreation of the coast of Lima by the purpose of evaluating the usefulness of Escherichia coli and Enterococcus like indicators of fecal pollution. In every month 5 series of samplings were done. They used like indicators of fecal pollution total Coliforms (CT) 5,000 NMP/100mL, fecal Coliforms (CF) 1,000 NMP/100mL according to the Peruvian Norm and also to Escherichia coli (EC) 100 NMP/100mL and Enterococcus (E) 35 NMP/100mL. The results of the analyses realized in January qualify to the water of 3 beaches like unacceptable for the bath, according to the Peruvian Norm; but it is used as indicator EC and E, 8 beaches would be considered to be like unacceptable. The results of July demonstrate that the water of 8 beaches was acceptable, according to the P...

Consumption of transgenic foods constitutes a potential health risk. However, in Peru there is a lack of updated and reliable information on the presence of transgenics in food and on the relevant data on their labeling; in the same way about the food consumed by animals for supply, whose products are going to be ingested by humans. Objetive. To determine the transgenicity, through the detection of the 35S promoter, in industrialized corn food products for human and animal consumption, which are marketed in Lima and to verify if the labeling mentions whether or not it contains transgenic sequences. Methods. 30 food samples for human consumption and 10 for consumption by animals for production were analyzed; and the labeling was revised. The Dneasy Mericon Food kit was used for DNA extraction, the Real Time-PCR method for P35S detection using the Mericon Screen 35S kit, and the Mericon Qu...

Introducción. El consumo de alimentos industrializados que contienen organismos genéticamente modificados (OGM) se ha incrementado notablemente. Desde su inicio ha generado crecientes controversias debido a que se considera de riesgo para la salud. En Perú se carece de información científica sobre los OGM en alimentos industrializados. Objetivo. Detectar y cuantificar molecularmente los indicadores de transgenicidad P35S y TNOS, y la soya transgénica Roundup Ready en alimentos industrializados de soya; y verificar su mención en la etiqueta. Métodos. Analizamos 30 muestras, para extraer el ADN utilizamos los kits Dneasy Mericon Food y Dneasy Power Soil. Para la detección y cuantificación de las secuencias transgénicas usamos la técnica PCR en tiempo real con los kits Mericon. Resultados. Detectamos transgenicidad en el 100% de las muestras y soya Roundup Ready en el 66,7%. El ...

This study explores the assessments made by the inhabitants of eight Latin American countries regarding the current state of the environment, at a local, national and global scale, and how it is perceived looking ahead to the future. It also examines the assignment of responsibility in the future management of the environment. A total of 944 people took part in the study from the eight participating countries, men and women over the age of 18 with different levels of education. The study uses the Environmental Futures Scale to which two relevant items have been added to assess the environment in the region, as well as the Assignment of Environmental Responsibility Scale. The results show differences between the countries, although in general they are pessimistic regarding the current and future state of the environment at the local, national and global level, with the exception of Brazil...