The polyethylene biodegradation by microorganisms is a solution for the reduction of the plastics pollution. Isolation and characterization of the fungi capable to degrade the polyethylene are reported, as well as the conditions of pH and temperature in which they showed the higher activity. Fungi were isolated from polyethylene products obtained of sanitary landfill; the identification was realized in base to growth in petri plate and the technique of microculture in sheet. Biodegradative activity was determined with the Kavelman and Kendrick technique, for temperatures between 20 and 30 °C and to pH 4,5 – 8,0. Twenty strains of micromicete were isolated and identified. Five strain (25%) shown polyethylene biodegradative capacity to 20 °C, and pH 6,5. Six strain (30%) showed activity biodegradative to 30 °C, and pH 6,5. In both cases, Aspergillus flavus was the strain with greater ...

The aim of the study was to isolate and characterize E. coli O157: H7 from fresh ground beef obtained in different food markets. 195 samples were analyzed, for isolation and enumeration of E. coliwas used most probable number technique using multiple tubes, for the isolation and characterization of E. coliO157: H7 selective enrichment and biochemical analysis, characteristic colonies were confirmed serologically. To determine the presence of shigatoxin (stx1, stx2) and intimin (eaeA) was done with multiplex real time PCR and for enterohemolysin the hemolysis test. The 87.18% of the samples were positive for E. coliand 77.95% had a count greater than or equal to 50 NMP/g. Were obtained 3 (1.54%) strains of E. coliO157: H7, one stx1 +/stx2 +/eaeA - and enterohemolysin -, one stx1 +/stx2 -/eaeA + and enterohemolysin - and the other stx1 -/stx2 -/eaeA - and enterohemolysin +. Also obtained 4...

The polyethylene biodegradation by microorganisms is a solution for the reduction of the plastics pollution. Isolation and characterization of the fungi capable to degrade the polyethylene are reported, as well as the conditions of pH and temperature in which they showed the higher activity. Fungi were isolated from polyethylene products obtained of sanitary landfill; the identification was realized in base to growth in petri plate and the technique of microculture in sheet. Biodegradative activity was determined with the Kavelman and Kendrick technique, for temperatures between 20 and 30 °C and to pH 4,5 – 8,0. Twenty strains of micromicete were isolated and identified. Five strain (25%) shown polyethylene biodegradative capacity to 20 °C, and pH 6,5. Six strain (30%) showed activity biodegradative to 30 °C, and pH 6,5. In both cases, Aspergillus flavus was the strain with greater ...

The aim of the study was to isolate and characterize E. coli O157: H7 from fresh ground beef obtained in different food markets. 195 samples were analyzed, for isolation and enumeration of E. coliwas used most probable number technique using multiple tubes, for the isolation and characterization of E. coliO157: H7 selective enrichment and biochemical analysis, characteristic colonies were confirmed serologically. To determine the presence of shigatoxin (stx1, stx2) and intimin (eaeA) was done with multiplex real time PCR and for enterohemolysin the hemolysis test. The 87.18% of the samples were positive for E. coliand 77.95% had a count greater than or equal to 50 NMP/g. Were obtained 3 (1.54%) strains of E. coliO157: H7, one stx1 +/stx2 +/eaeA - and enterohemolysin -, one stx1 +/stx2 -/eaeA + and enterohemolysin - and the other stx1 -/stx2 -/eaeA - and enterohemolysin +. Also obtained 4...