acceptable, then detect the pollution source. It was performed a microbiologicalanalysis of the water of 21 Lima’s beaches and the detections of contamination sources with human feces. The results qualify the water of 33.33% (07) beaches as unacceptable if it is used as indicators of fecal pollution, the total coliforms and fecal coliforms; if it is included Escherichia coli, it reaches to 47.62% (10); and if also it is included Enterococcus it reaches to 66.66%(14). It was demonstrated the existence of domestic sewage discharge in all the beaches considered as unacceptable; in 35.75% (05) with visual inspection; in 14.29% (02) was observed water running with fecal pollution and in 50% (07) by the relation between E. coli and Enterococcus.

In July (winter) of 2005 and January (summer) of 2006 there was realized the microbiological study of the water of 8 beaches of summery recreation of the coast of Lima by the purpose of evaluating the usefulness of Escherichia coli and Enterococcus like indicators of fecal pollution. In every month 5 series of samplings were done. They used like indicators of fecal pollution total Coliforms (CT) 5,000 NMP/100mL, fecal Coliforms (CF) 1,000 NMP/100mL according to the Peruvian Norm and also to Escherichia coli (EC) 100 NMP/100mL and Enterococcus (E) 35 NMP/100mL. The results of the analyses realized in January qualify to the water of 3 beaches like unacceptable for the bath, according to the Peruvian Norm; but it is used as indicator EC and E, 8 beaches would be considered to be like unacceptable. The results of July demonstrate that the water of 8 beaches was acceptable, according to the P...

To detect injured coliforms (IC) in drinking water considered suitable for the human consumption according to the Peruvian normativity, were taken and processed 100 water samples of buildings of the district of Lima Cercado. For the analysis the residual free chlorine (RFC) by means of DPD technique and pH was determined using indicating rods Merck. For the count of heterotrophic bacteria (HB) the spread plate method was used, to quantify total Coliform (TC) and fecal Coliform (FC) the techniques of multiple tubes and membrane fi lter were used. In order to recover and to enumerate injured TC and FC the m-T7 medium were used. For the bacterial specifi cation the standard tests and API 20E system. For CI’s detection there were in used the samples that turned out to be negative for TC and FC and/or had count of HB an equal or lower to 500 UFC/mL. Using the conventional methods was demons...

To detect injured coliforms (IC) in drinking water considered suitable for the human consumption according to the Peruvian normativity, were taken and processed 100 water samples of buildings of the district of Lima Cercado. For the analysis the residual free chlorine (RFC) by means of DPD technique and pH was determined using indicating rods Merck. For the count of heterotrophic bacteria (HB) the spread plate method was used, to quantify total Coliform (TC) and fecal Coliform (FC) the techniques of multiple tubes and membrane fi lter were used. In order to recover and to enumerate injured TC and FC the m-T7 medium were used. For the bacterial specifi cation the standard tests and API 20E system. For CI’s detection there were in used the samples that turned out to be negative for TC and FC and/or had count of HB an equal or lower to 500 UFC/mL. Using the conventional methods was demons...

The aim of the study was to isolate and characterize E. coli O157: H7 from fresh ground beef obtained in different food markets. 195 samples were analyzed, for isolation and enumeration of E. coliwas used most probable number technique using multiple tubes, for the isolation and characterization of E. coliO157: H7 selective enrichment and biochemical analysis, characteristic colonies were confirmed serologically. To determine the presence of shigatoxin (stx1, stx2) and intimin (eaeA) was done with multiplex real time PCR and for enterohemolysin the hemolysis test. The 87.18% of the samples were positive for E. coliand 77.95% had a count greater than or equal to 50 NMP/g. Were obtained 3 (1.54%) strains of E. coliO157: H7, one stx1 +/stx2 +/eaeA - and enterohemolysin -, one stx1 +/stx2 -/eaeA + and enterohemolysin - and the other stx1 -/stx2 -/eaeA - and enterohemolysin +. Also obtained 4...

The aim of the study was to isolate and characterize E. coli O157: H7 from fresh ground beef obtained in different food markets. 195 samples were analyzed, for isolation and enumeration of E. coliwas used most probable number technique using multiple tubes, for the isolation and characterization of E. coliO157: H7 selective enrichment and biochemical analysis, characteristic colonies were confirmed serologically. To determine the presence of shigatoxin (stx1, stx2) and intimin (eaeA) was done with multiplex real time PCR and for enterohemolysin the hemolysis test. The 87.18% of the samples were positive for E. coliand 77.95% had a count greater than or equal to 50 NMP/g. Were obtained 3 (1.54%) strains of E. coliO157: H7, one stx1 +/stx2 +/eaeA - and enterohemolysin -, one stx1 +/stx2 -/eaeA + and enterohemolysin - and the other stx1 -/stx2 -/eaeA - and enterohemolysin +. Also obtained 4...

In July (winter) of 2005 and January (summer) of 2006 there was realized the microbiological study of the water of 8 beaches of summery recreation of the coast of Lima by the purpose of evaluating the usefulness of Escherichia coli and Enterococcus like indicators of fecal pollution. In every month 5 series of samplings were done. They used like indicators of fecal pollution total Coliforms (CT) 5,000 NMP/100mL, fecal Coliforms (CF) 1,000 NMP/100mL according to the Peruvian Norm and also to Escherichia coli (EC) 100 NMP/100mL and Enterococcus (E) 35 NMP/100mL. The results of the analyses realized in January qualify to the water of 3 beaches like unacceptable for the bath, according to the Peruvian Norm; but it is used as indicator EC and E, 8 beaches would be considered to be like unacceptable. The results of July demonstrate that the water of 8 beaches was acceptable, according to the P...