The objective of the present study was to determine the presence of Trypanosoma sp. in collared peccaries reared in captivity in Iquitos and Moyobamba area. Blood samples were collected from 40 animals from 3 months to 2 years of age. Samples were processed by the microcapillary or Woo technique and the blood smear. None of the samples resulted positive to Trypanosoma sp., and probably the low sensitivity of the techniques affected the results. The Monte Carlo risk analysis (@Risk) indicated that the probability to obtain an infected animal in the Moyobamba and Iquitos area was 0.02% and that 95% of the samples were within an interval of 0.006 a 0.854%.

El objetivo del presente estudio fue determinar la presencia de Trypanosoma sp. en sajinos en cautiverio de las zonas de Iquitos y Moyobamba. Se colectó muestras de sangre de 40 sajinos procedentes de tres zoocriaderos con edades entre 3 meses y 2 años. Las muestras se procesaron con las técnicas de microcapilar o técnica de Woo y del frotis sanguíneo delgado. No se halló la presencia de Trypanosoma sp. en las muestras examinadas, y, posiblemente, la baja sensibilidad de las técnicas afectó los resultados. La técnica de evaluación de riesgo por simulación Monte Carlo (programa @Risk) indicó que el 95% de las observaciones analizadas se encontraron en un intervalo de 0.006 a 0.854% y que la probabilidad de encontrar la infección real en sajinos provenientes de Iquitos y Moyobamba se encuentra en un rango promedio de infección de 0.02%.

The objective of the present study was to evaluate the presence of Cryptosporidium parvum as a risk factor to the occurrence of diarrhea in alpacas younger than 15 days of age from peasant communities in the area of Cusco, using a case–control study design. Stool samples were collected from animals with (n=248) and without diarrhea (n=231) during the 2006 calving season. The presence of C. parvum was determined by the Modified Ziehl Neelsen stain technique (MZN). The results were analyzed using a logistic regression model, adjusting potentially confusing variables as age, sex, breed and location of alpacas. The Odds Ratio was 4.3 (C.I. = 2.3–7.9). The study demonstrated that neonatal alpaca positives to C. parvum have 4.3 times higher predisposition to suffer diarrheas in relation to the animals negative to MZN. In addition, significant association was observed between the animals MZ...

El presente estudio tuvo como objetivo evaluar si la presencia de Cryptosporidium parvum es un factor de riesgo de diarrea en alpacas neonatas menores de 15 días de edad, provenientes de diversas unidades alpaqueras del departamento de Cusco. Se empleó el diseño epidemiológico de Caso-Control para establecer si existe una relación causal. Se tomaron 248 y 231 muestras fecales de animales con y sin diarrea, respectivamente, durante la temporada de parición del 2006. Se determinó la presencia de C. parvum mediante le técnica de tinción de Ziehl Neelsen Modificado (ZNM). Los datos se analizaron empleando una regresión logística, que ajustó variables potencialmente confundentes como edad, sexo, raza y lugar de origen de las alpacas. Se encontró un Odds Ratio de 4.3 (I.C. = 2.3–7.9). El estudio demuestra que las alpacas positivas a C. parvum tienen 4.3 veces mayor predisposici�...

The role of Cryptosporidium parvum as a risk factor for the occurrence of neonatal diarrhea was evaluated using a case-control study design in alpacas from Puno. The study was carried out during the calving season, between February and March 2006. Stool samples (n=487) were collected in alpacas of 1 to 15 days of age in four localities: Antacalla, La Raya, Quimsachata and Macusani. The faecal smears were processed following the Modified Ziehl-Neelsen stain. The 39% (130/336) of alpacas with diarrhea and 23% (35/151) of alpacas without diarrhea were positives to C. parvum infection. The logistic regression showed that this parasite does not represent a risk factor for neonatal diarrhea in the screened animals (OR: 1.5, IC 95%: 0.9-2.4). It was also determined that La Raya was statistically significant (p=0.046) and represented a risk factor for the occurrence of neonatal diarrhea (OR: 2.5...

El presente trabajo evaluó en campo el rol del Cryptosporidium parvum como factor de riesgo en la presentación de diarrea neonatal empleando el diseño epidemiológico de Caso-Control en alpacas del departamento de Puno. El muestreo se realizó durante la temporada de parición, entre los meses de febrero y marzo de 2006. Se recolectaron muestras fecales (n=487) de alpacas entre 1 a 15 días de edad procedentes de las localidades de Antacalla, La Raya, Quimsachata y Macusani. Los frotis fecales, fijados previamente en metanol, fueron procesados según la técnica de tinción de Ziehl-Neelsen Modificado. El 39% (130/336) de los animales con diarrea y el 23% (35/151) de las que estaban sin diarrea fueron positivos a la infección por C. parvum. El análisis de regresión logística demostró que no hubo relación estadística significativa entre la presencia de Cryptosporidium y la diarr...

The aim of the study was to establish whether the presence of Cryptosporidium parvum in the dam is a risk factor for the presentation of the pathogen in the baby alpaca. The study included 698 alpaca dams and their offspring less than 30 days of age. The sampling took place from January till March 2007 in the localities of La Raya, Choquecota, Chillihua, Maranganí and Silli in Canchis province, Cusco, Peru. Fecal samples were collected from the rectum, processed using the Modified Ziehl-Neelsen stain and observed by microscopy. Risk analysis was done through logistic regressions. Results showed that a baby alpaca born from a positive dam to C. parvum was 2.1 times more likely to get infected with the parasite as compared with others born from a negative dam (p<0.05). Likewise, sex and presence of diarrhea were not significant, whereas place of breeding showed to be a protection facto...

The aim of the study was to establish whether the presence of Cryptosporidium parvum in the dam is a risk factor for the presentation of the pathogen in the baby alpaca. The study included 698 alpaca dams and their offspring less than 30 days of age. The sampling took place from January till March 2007 in the localities of La Raya, Choquecota, Chillihua, Maranganí and Silli in Canchis province, Cusco, Peru. Fecal samples were collected from the rectum, processed using the Modified Ziehl-Neelsen stain and observed by microscopy. Risk analysis was done through logistic regressions. Results showed that a baby alpaca born from a positive dam to C. parvum was 2.1 times more likely to get infected with the parasite as compared with others born from a negative dam (p<0.05). Likewise, sex and presence of diarrhea were not significant, whereas place of breeding showed to be a protection facto...

The aim of this study was to detect Anaplasma phagocytophilum in horses from the province of Chiclayo, Peru, using haematological techniques. One hundred blood samples were collected from apparently healthy horses with a history of exposure to ticks. The haematological study consisted of blood count and the evaluation of blood smears stained with Wright and acridine orange to search for inclusion bodies and morulae of the pathogen. Results showed that 9% of horses (9/100) presented structures compatible with inclusion bodies or morulae of A. phagocytophilum, so they were considered as "positive". This group of animals presented slight alterations in the leukocytic and thrombocytic series. The findings indicate the presence of A. phagocytophilum in horses in Peru.

The aim of this study was to detect Anaplasma phagocytophilum in horses from the province of Chiclayo, Peru, using haematological techniques. One hundred blood samples were collected from apparently healthy horses with a history of exposure to ticks. The haematological study consisted of blood count and the evaluation of blood smears stained with Wright and acridine orange to search for inclusion bodies and morulae of the pathogen. Results showed that 9% of horses (9/100) presented structures compatible with inclusion bodies or morulae of A. phagocytophilum, so they were considered as "positive". This group of animals presented slight alterations in the leukocytic and thrombocytic series. The findings indicate the presence of A. phagocytophilum in horses in Peru.