Blastocystis is a chromist that can be found in the intestinal tract of animals and humans, and is currently considered an emerging pathogen with zoonotic potential. The aim of this study was to compare the efficacy of three in vitro media culture which are considered appropriate for the development of Blastocystis: Jones, Pavlova and Boeck-Drbohlav modified (BDM). It was used 54 stool samples from Blastocystis positive pigs diagnosed by the spontaneous sedimentation technique. The number of microorganisms inoculated was divided in three categories: low, intermediate and high. Each sample was cultivated for duplicate in the three media and after 72 hours of incubation the pathogens were counted using the Neubauer chamber. Results showed that the Jones media was the more efficient, followed by Pavlova and BDM; moreover, better results were obtained when the level of microorganisms was low...

Blastocystis is a chromist that can be found in the intestinal tract of animals and humans, and is currently considered an emerging pathogen with zoonotic potential. The aim of this study was to compare the efficacy of three in vitro media culture which are considered appropriate for the development of Blastocystis: Jones, Pavlova and Boeck-Drbohlav modified (BDM). It was used 54 stool samples from Blastocystis positive pigs diagnosed by the spontaneous sedimentation technique. The number of microorganisms inoculated was divided in three categories: low, intermediate and high. Each sample was cultivated for duplicate in the three media and after 72 hours of incubation the pathogens were counted using the Neubauer chamber. Results showed that the Jones media was the more efficient, followed by Pavlova and BDM; moreover, better results were obtained when the level of microorganisms was low...

The aim of the study was to establish whether the presence of Cryptosporidium parvum in the dam is a risk factor for the presentation of the pathogen in the baby alpaca. The study included 698 alpaca dams and their offspring less than 30 days of age. The sampling took place from January till March 2007 in the localities of La Raya, Choquecota, Chillihua, Maranganí and Silli in Canchis province, Cusco, Peru. Fecal samples were collected from the rectum, processed using the Modified Ziehl-Neelsen stain and observed by microscopy. Risk analysis was done through logistic regressions. Results showed that a baby alpaca born from a positive dam to C. parvum was 2.1 times more likely to get infected with the parasite as compared with others born from a negative dam (p<0.05). Likewise, sex and presence of diarrhea were not significant, whereas place of breeding showed to be a protection facto...

The aim of the study was to establish whether the presence of Cryptosporidium parvum in the dam is a risk factor for the presentation of the pathogen in the baby alpaca. The study included 698 alpaca dams and their offspring less than 30 days of age. The sampling took place from January till March 2007 in the localities of La Raya, Choquecota, Chillihua, Maranganí and Silli in Canchis province, Cusco, Peru. Fecal samples were collected from the rectum, processed using the Modified Ziehl-Neelsen stain and observed by microscopy. Risk analysis was done through logistic regressions. Results showed that a baby alpaca born from a positive dam to C. parvum was 2.1 times more likely to get infected with the parasite as compared with others born from a negative dam (p<0.05). Likewise, sex and presence of diarrhea were not significant, whereas place of breeding showed to be a protection facto...