Blastocystis is a chromist that can be found in the intestinal tract of animals and humans, and is currently considered an emerging pathogen with zoonotic potential. The aim of this study was to compare the efficacy of three in vitro media culture which are considered appropriate for the development of Blastocystis: Jones, Pavlova and Boeck-Drbohlav modified (BDM). It was used 54 stool samples from Blastocystis positive pigs diagnosed by the spontaneous sedimentation technique. The number of microorganisms inoculated was divided in three categories: low, intermediate and high. Each sample was cultivated for duplicate in the three media and after 72 hours of incubation the pathogens were counted using the Neubauer chamber. Results showed that the Jones media was the more efficient, followed by Pavlova and BDM; moreover, better results were obtained when the level of microorganisms was low...

Blastocystis is a chromist that can be found in the intestinal tract of animals and humans, and is currently considered an emerging pathogen with zoonotic potential. The aim of this study was to compare the efficacy of three in vitro media culture which are considered appropriate for the development of Blastocystis: Jones, Pavlova and Boeck-Drbohlav modified (BDM). It was used 54 stool samples from Blastocystis positive pigs diagnosed by the spontaneous sedimentation technique. The number of microorganisms inoculated was divided in three categories: low, intermediate and high. Each sample was cultivated for duplicate in the three media and after 72 hours of incubation the pathogens were counted using the Neubauer chamber. Results showed that the Jones media was the more efficient, followed by Pavlova and BDM; moreover, better results were obtained when the level of microorganisms was low...

In Peru there is clinical evidence of the Influenza A virus (VIA) and the presence of antibodies against the VIA in pigs, but there are no official reports of frequency in pig farms in the country. The aim of the study was to determine the frequency of antibodies against VIA in technical farms in Lima, Ica and Arequipa with a history of respiratory problems. Blood samples were obtained from eight age groups of pigs during 2020-2021. Antibodies against VIA were detected using a blocking ELISA test (IDEXX, USA). A total of 283 serum samples from Lima, 287 from Ica and 288 from Arequipa were processed. The overall frequency of antibodies against VIA was 24.9% (95% CI 22.0 – 27.8); while in Lima, Ica and Arequipa it was 33.2% (95% CI 27.8 - 39.0), 19.5% (95% CI 15.1 - 24.6) and 22.2% (95% CI 17.6 - 27.5), respectively. The logistic regression analysis showed that in the Lima region, the ag...