Bartonella bacilliformis is the etiologic agent of Carrion's disease, which if endemic to Peru. Studies on antimicrobial resistance genes from clinical isolates of this pathogen are scarce, and the molecular characteristics of these genes and their region resistance-associated are currently unknown. In this work we made the molecular characterization of the quinolone-resistance, and establish the region (QRDR) for the topoisomerase IV, which is encoded by the parC and parE genes, as well as develop an antimicrobial susceptibility test for B. bacilliformis. 65 Blood samples from La Libertad, Cusco, Ancash and Piura were processed on Blood Agar plates and incubated at 30 °C, 5% CO2. The antimicrobial susceptibility was determined, then the genomic DNA extracted, aforementioned genes amplified, their sequence determined and it analyzed using bioinformatics tools. Six positive cultures were...

Bartonella bacilliformis is the etiologic agent of Carrion's disease, which if endemic to Peru. Studies on antimicrobial resistance genes from clinical isolates of this pathogen are scarce, and the molecular characteristics of these genes and their region resistance-associated are currently unknown. In this work we made the molecular characterization of the quinolone-resistance, and establish the region (QRDR) for the topoisomerase IV, which is encoded by the parC and parE genes, as well as develop an antimicrobial susceptibility test for B. bacilliformis. 65 Blood samples from La Libertad, Cusco, Ancash and Piura were processed on Blood Agar plates and incubated at 30 °C, 5% CO2. The antimicrobial susceptibility was determined, then the genomic DNA extracted, aforementioned genes amplified, their sequence determined and it analyzed using bioinformatics tools. Six positive cultures were...

Toll-like receptors (TLRs) are transmembrane proteins that are key regulators of innate and adaptive immune responses, particularly TLR4, and they have been identified as potential drug targets for the treatment of disease. Several low-molecular-weight compounds are being considered as new drug targets for various applications, including as immune modulators. Mygalin, a 417 Da synthetic bis-acylpolyamine, is an analog of spermidine that has microbicidal activity. In this study, we investigated the effect of mygalin on the innate immune response based on a virtual screening (VS) and molecular docking analysis. Bone marrow-derived macrophages and the cell lines J774A.1 and RAW 264.7 stimulated with lipopolysaccharide (LPS) were used to confirm the data obtained in silico. Virtual screening and molecular docking suggested that mygalin binds to TLR4 via the protein myeloid differentiation fa...

Inappropriate use of antibiotics favors the selection and spread of resistant bacteria. To reduce the spread of these bacteria, finding new molecules with activity is urgent and necessary. Several polyamine analogs have been constructed and used to control microorganisms and tumor cells. Mygalin is a synthetic acylpolyamine, which are analogs of spermidine, derived from the hemolymph of the spider Acanthoscurria gomesiana. The effective activity of polyamines and their analogs has been associated with their structure. The presence of two acyl groups in the Mygalin structure may give this molecule a specific antibacterial activity. The aim of this study was to identify the mechanisms involved in the interaction of Mygalin with Escherichia coli to clarify its antimicrobial action. The results indicated that Mygalin exhibits intense dose and time-dependent bactericidal activity. Treatment o...

PROCYT N°323-2010- CONCYTEC-OAJ

Bartonella bacilliformis, agente causal de la Enfermedad de Carrión transmitida por insectos flebotominos del género Lutzomyia y otros, es una endemia ancestral que afecta especialmente a la población más pobre de nuestros valles interandinos. En el Perú está presente en 12 de 25 departamentos. Es endémica en Ancash, Cajamarca, Amazonas, Cusco, La Libertad y Piura. Pocas son las investigaciones acerca de la susceptibilidad in vitro a antimicrobianos de Bartonella bacilliformis, no se cuenta con una prueba estandarizada de antibiograma para esta bacteria, como tampoco se conocen los mecanismos de resistencia ni las secuencias de los genes asociados a dicha resistencia. Por consiguiente, se ha planteado evaluar la resistencia antimicrobiana in vitro de cepas de Bartonella bacilliformis aisladas de zonas endémicas, mediante métodos convencionales y métodos moleculares, con la fina...

Carrion's disease, caused by the bacterium Bartonella bacilliformis, is a serious public health problem in Peru, Ecuador and Colombia. Currently there is no available vaccine against B. bacilliformis. While antibiotics are the standard treatment, resistant strains have been reported, and there is a potential spread of the vector that transmits the bacteria. This study aimed to design a multi-epitope vaccine candidate against the causative agent of Carrion's disease using immunoinformatics tools. Predictions of B-cell epitopes, as well as CD4+ and CD8+T cell epitopes, were performed from the entire proteome of B. bacilliformis KC583 using the most frequent alleles from Peru, Ecuador, Colombia, and worldwide. B-cell epitopes and T-cell nested epitopes from outer membrane and virulence-associated proteins were selected. Epitopes were filtered out based on promiscuity, non-allergenicity, con...

Abstract We designed a 16S rRNA gene PCR-RFLP scheme to identify all currently described Bartonella spp. The 16S rRNA genes of all Bartonella spp. were in-silico analyzed in order to design a RFLP technique able to discriminate among different species. The restriction enzymes selected were MaeIII, MseI, Sau96I, BsaAI, DrdI, FokI, BssHII, BstUI, AluI, TspDTI and HphI which, according to a decision-making tree, facilitated the differentiation of all the currently described species of Bartonella.The technique was experimentally tested in different species of Bartonella, including human pathogenic B. bacilliformis and B. henselae with a 100% of concordance with the in-silico predicted patterns.This novel RFLP assay could be used to identify both human and non-human pathogenic Bartonella in diagnostic, phylogenetic and epidemiologic studies.