Se evaluó la acción de 4 químicos bactericidas, utilizados en la industria de harina de pescado, frente a Salmonella choleraesuis, Escherichia coli, Proteus hauseri y Shigella sonnei. Los químicos bactericidas que contenían ácidos orgánicos: propiónico y fórmico (N1 y N2), propiónico y formaldehído (N3), propiónico, fórmico, acético y formol (N4) fueron evaluados por dos métodos a fi n de determinar su efectividad y efi cacia respectivamente. En el método 1 “difusión de discos bactericidas en agar”, el diámetro de la zona de inhibición bacteriana fue determinado a las 24, 48 y 72 h. En el método 2 “bactericidas en suspensión”, cada bacteria fue adicionada por separado a tubos conteniendo bactericida a la dosis recomendada, incubando de 1 a 7 días; se evaluó la resistencia bacteriana. En el primer método, sólo el bactericida N2 no mostró efectividad fre...

Dentro del objetivo del siguiente estudio se intenta Identificar el nivel de Síndrome de Burnout en el personal de salud del Centro de Salud de Sauces, Guayaquil, Ecuador 2020, este estudio es una investigación de tipo descriptivo simple cuantitativo de corte transversal , se realizó una encuesta a 60 personas que trabajan en el centro de salud de Sauces en la cual se les realizo la aplicación del test de Maslach Burnout Inventory teniendo como resultado un nivel de síndrome de burnout de nivel medio a nivel alto, 53% de nivel medio y un 47% de nivel alto, en la dimensión de cansancio emocional un 51,7% alto , en el de despersonalización el 45% medio y el de realización personal tiene un porcentaje de 55% que constituye un nivel alto, llegando a la conclusión que el Centro de Salud presenta un nivel medio a alto de Síndrome de Burnout. Conclusión: se identifico que el personal...

Bacteria and microbial enzymes are biocatalysts and can be used as an alternative to industrial chemical processes. The present study focused on isolating and identifying bacterial strains from shrimp waste, that produce amylases, lipases, proteases and chitinases with potential use on shrimp waste treatment. Thirty-two bacterial strains were isolated, phenotypically characterized, and identified by the API system and the molecular analysis of the 16S rDNA. It was found that 28.13% of the isolated bacterial strains had amylolytic capacity, 87.50% lipolytic, 96.88% proteolytic and 28.13% chitinolytic capacity on agar plates with specific substrates. The genera Bacillus, Burkholderia, Ochrobactrum, Vibrio, Pseudomonas and Shewanella were identified. Bacteria with enzymatic capacities isolated in the present study, could be used to obtain by-products from shrimp waste as well as other indus...

Bacteria and microbial enzymes are biocatalysts and can be used as an alternative to industrial chemical processes. The present study focused on isolating and identifying bacterial strains from shrimp waste, that produce amylases, lipases, proteases and chitinases with potential use on shrimp waste treatment. Thirty-two bacterial strains were isolated, phenotypically characterized, and identified by the API system and the molecular analysis of the 16S rDNA. It was found that 28.13% of the isolated bacterial strains had amylolytic capacity, 87.50% lipolytic, 96.88% proteolytic and 28.13% chitinolytic capacity on agar plates with specific substrates. The genera Bacillus, Burkholderia, Ochrobactrum, Vibrio, Pseudomonas and Shewanella were identified. Bacteria with enzymatic capacities isolated in the present study, could be used to obtain by-products from shrimp waste as well as other indus...

Bartonella bacilliformis is the etiologic agent of Carrion's disease, which if endemic to Peru. Studies on antimicrobial resistance genes from clinical isolates of this pathogen are scarce, and the molecular characteristics of these genes and their region resistance-associated are currently unknown. In this work we made the molecular characterization of the quinolone-resistance, and establish the region (QRDR) for the topoisomerase IV, which is encoded by the parC and parE genes, as well as develop an antimicrobial susceptibility test for B. bacilliformis. 65 Blood samples from La Libertad, Cusco, Ancash and Piura were processed on Blood Agar plates and incubated at 30 °C, 5% CO2. The antimicrobial susceptibility was determined, then the genomic DNA extracted, aforementioned genes amplified, their sequence determined and it analyzed using bioinformatics tools. Six positive cultures were...

Bartonella bacilliformis is the etiologic agent of Carrion's disease, which if endemic to Peru. Studies on antimicrobial resistance genes from clinical isolates of this pathogen are scarce, and the molecular characteristics of these genes and their region resistance-associated are currently unknown. In this work we made the molecular characterization of the quinolone-resistance, and establish the region (QRDR) for the topoisomerase IV, which is encoded by the parC and parE genes, as well as develop an antimicrobial susceptibility test for B. bacilliformis. 65 Blood samples from La Libertad, Cusco, Ancash and Piura were processed on Blood Agar plates and incubated at 30 °C, 5% CO2. The antimicrobial susceptibility was determined, then the genomic DNA extracted, aforementioned genes amplified, their sequence determined and it analyzed using bioinformatics tools. Six positive cultures were...

Abstract We designed a 16S rRNA gene PCR-RFLP scheme to identify all currently described Bartonella spp. The 16S rRNA genes of all Bartonella spp. were in-silico analyzed in order to design a RFLP technique able to discriminate among different species. The restriction enzymes selected were MaeIII, MseI, Sau96I, BsaAI, DrdI, FokI, BssHII, BstUI, AluI, TspDTI and HphI which, according to a decision-making tree, facilitated the differentiation of all the currently described species of Bartonella.The technique was experimentally tested in different species of Bartonella, including human pathogenic B. bacilliformis and B. henselae with a 100% of concordance with the in-silico predicted patterns.This novel RFLP assay could be used to identify both human and non-human pathogenic Bartonella in diagnostic, phylogenetic and epidemiologic studies.