Four samples of silts were collected from the bottom and the bank of the beach “Marbella” in Callao and four samples of cultivated land were taken at level of rhizosphere obtained from a vineyard of “Grocio Prado” Grassland’s town in the Country of Chincha. The microbial heterotrophics populations with chitinolytic activity present in these environments was determined quantitatively, being described its main phenotypic characteristics. For the quantification of the populations the method of the Most Probable Number (NMP) was used. The tubes with positive growth were sowed in tubes with Granulated Chitin Agar and Colloidal Chitin Agar that were incubated at environmental temperature for two weeks. With the developed colonies the following tests were carried out: Oxidation/Fermentation of Glucose, Oxidase, Catalase, motility, presence of flagellum, tintoreal capacity, production ...

Four samples of silts were collected from the bottom and the bank of the beach “Marbella” in Callao and four samples of cultivated land were taken at level of rhizosphere obtained from a vineyard of “Grocio Prado” Grassland’s town in the Country of Chincha. The microbial heterotrophics populations with chitinolytic activity present in these environments was determined quantitatively, being described its main phenotypic characteristics. For the quantification of the populations the method of the Most Probable Number (NMP) was used. The tubes with positive growth were sowed in tubes with Granulated Chitin Agar and Colloidal Chitin Agar that were incubated at environmental temperature for two weeks. With the developed colonies the following tests were carried out: Oxidation/Fermentation of Glucose, Oxidase, Catalase, motility, presence of flagellum, tintoreal capacity, production ...

Objective: To assess in vitro sensitivity / resistance of biofilms and planktonic populations of Pseudomonas aeruginosa from a hospital environment to ciprofloxacin and to compare them against the results of standard susceptibility test obtained. Design: Microbiological study. Setting: Molecular Microbiology Laboratory, Faculty of Biological Sciences, UNMSM. Biologic material: Pseudomonas aeruginosa strains. Interventions: Resistant and susceptible strains obtained form Hospital del Niño were tested for minimum inhibitory concentration (MIC); then biofilms were formed on filtration membranes, and planktonic cultures in broth were tested with different ciprofloxacin concentrations, with plate count at 40 minutes intervals and 24 hours after exposure. Main outcome measures: In vitro susceptibility / resistance of hospital origin Pseudomonas aeruginosa biofilms and planktonic populations. ...

OBJECTIVE: Isolation and identification of Vibrio cholerae O1 and non-epidemic Vibrio species associated with acute diarrhea (AD) during the 1998 "El Niño" Southern Oscillation (ENSO). MATERIAL AND METHODS: During 1998 summer months, 70 stool samples from AD patients admitted in Hospital Nacional Dos de Mayo emergency room of Lima were cultured; TCBS Agar isolates were studied. Biochemical and serological tests were performed for identification of Vibrio cholerae O1. Non-epidemic vibrios and others pathogenic vibrios identification was performed according to Bergey’s Bacterial Systematic Manual (1994). RESULTS: Most cases were associated to Vibrio cholerae O1 as unique etiologic agent (64,3%) or related to the others Vibrio species (4,2%). Two cases involving Vibrio vulnificus (2,9%) and 3 with V. parahaemolyticus (4,3%) as etiologic agents of AD, are described. CONCLUSIONS: Vibrio ch...

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This work was carried out in 1988 during summer and autumn seasons 122 samples were tested; 88 from frozen fish, 40 from "cebiche" marketed in Lima. To processing and to isolate the halophilic vibri'On it was followed the suggestion of FDA (1985) characterization of ,the isolates were done on morphological and cultural characteristics and biochemical tests 57 presumptive strains were isolated 42 from frozen fish and 15 from "cebiches", 5 strains Were identified as Vibrio parahaemolyticus (8.8%) and 14 strains like Vibrio aIginolyticus (24.6'0). 38 strains were characterized as Vibrio sp. (66.6%).

This work was carried out in 1988 during summer and autumn seasons 122 samples were tested; 88 from frozen fish, 40 from "cebiche" marketed in Lima. To processing and to isolate the halophilic vibri'On it was followed the suggestion of FDA (1985) characterization of ,the isolates were done on morphological and cultural characteristics and biochemical tests 57 presumptive strains were isolated 42 from frozen fish and 15 from "cebiches", 5 strains Were identified as Vibrio parahaemolyticus (8.8%) and 14 strains like Vibrio aIginolyticus (24.6'0). 38 strains were characterized as Vibrio sp. (66.6%).

The aim of this study was to determine if chicken meat stalls are sources of contamination with shigatoxigenic Escherichia coli (STEC) in Lima, Peru. Swabs from the surfaces of hands, cutting boards and sale tables of 50 chicken meat stalls in a large market in the district of San Juan de Miraflores, Lima, Peru were taken (n=150 samples). Standard microbiological isolation and molecular identification of stx1, stx2 and eaeA genes by PCR was performed. Results showed that 42% (63/150) and 25.3% (38/150) of samples were positive for E. coli and STEC respectively. Besides, 84% (42/50) and 66% (33/50) of chicken meat stalls had at least one contaminated surface with E. coli and STEC respectively. Also, 68.3% (43/63) of strains of E. coli isolated were pathogenic for presenting at least one of the evaluated genes. There were 38 STEC strains and presented stx1 (19.0%, 12/63), stx2 (14.3%, 9/63...

El objetivo del presente trabajo fue determinar si los puestos de venta de carne de pollo son una fuente de contaminación con Escherichia coli shigatoxigénica (STEC) en mercados de abastos. Se tomaron hisopados de la superficie de manos, tablas de picar y mesas de expendio de 50 puestos de expendio de carne de pollo en el distrito de San Juan de Miraflores, Lima, Perú (n=150 muestras). Se realizó aislamiento microbiológico estándar e identificación molecular de los genes stx1, stx2 y eaeA mediante PCR. El 42% (63/150) y 25.3% (38/150) de las muestras fueron positivas a E. coli y STEC, respectivamente. El 84% (42/50) y 66% (33/50) de los puestos de venta poseían al menos una de las superficies contaminadas con E. coli y STEC, respectivamente. El 68.3% (43/63) de las cepas de E. coli aisladas fueron patógenas por presentar al menos un gen evaluado. De estas, 38 cepas fueron STEC y...

Bartonella bacilliformis is the etiologic agent of Carrion's disease, which if endemic to Peru. Studies on antimicrobial resistance genes from clinical isolates of this pathogen are scarce, and the molecular characteristics of these genes and their region resistance-associated are currently unknown. In this work we made the molecular characterization of the quinolone-resistance, and establish the region (QRDR) for the topoisomerase IV, which is encoded by the parC and parE genes, as well as develop an antimicrobial susceptibility test for B. bacilliformis. 65 Blood samples from La Libertad, Cusco, Ancash and Piura were processed on Blood Agar plates and incubated at 30 °C, 5% CO2. The antimicrobial susceptibility was determined, then the genomic DNA extracted, aforementioned genes amplified, their sequence determined and it analyzed using bioinformatics tools. Six positive cultures were...

Bartonella bacilliformis is the etiologic agent of Carrion's disease, which if endemic to Peru. Studies on antimicrobial resistance genes from clinical isolates of this pathogen are scarce, and the molecular characteristics of these genes and their region resistance-associated are currently unknown. In this work we made the molecular characterization of the quinolone-resistance, and establish the region (QRDR) for the topoisomerase IV, which is encoded by the parC and parE genes, as well as develop an antimicrobial susceptibility test for B. bacilliformis. 65 Blood samples from La Libertad, Cusco, Ancash and Piura were processed on Blood Agar plates and incubated at 30 °C, 5% CO2. The antimicrobial susceptibility was determined, then the genomic DNA extracted, aforementioned genes amplified, their sequence determined and it analyzed using bioinformatics tools. Six positive cultures were...

Abstract We designed a 16S rRNA gene PCR-RFLP scheme to identify all currently described Bartonella spp. The 16S rRNA genes of all Bartonella spp. were in-silico analyzed in order to design a RFLP technique able to discriminate among different species. The restriction enzymes selected were MaeIII, MseI, Sau96I, BsaAI, DrdI, FokI, BssHII, BstUI, AluI, TspDTI and HphI which, according to a decision-making tree, facilitated the differentiation of all the currently described species of Bartonella.The technique was experimentally tested in different species of Bartonella, including human pathogenic B. bacilliformis and B. henselae with a 100% of concordance with the in-silico predicted patterns.This novel RFLP assay could be used to identify both human and non-human pathogenic Bartonella in diagnostic, phylogenetic and epidemiologic studies.