The aim of this study was to determine if chicken meat stalls are sources of contamination with shigatoxigenic Escherichia coli (STEC) in Lima, Peru. Swabs from the surfaces of hands, cutting boards and sale tables of 50 chicken meat stalls in a large market in the district of San Juan de Miraflores, Lima, Peru were taken (n=150 samples). Standard microbiological isolation and molecular identification of stx1, stx2 and eaeA genes by PCR was performed. Results showed that 42% (63/150) and 25.3% (38/150) of samples were positive for E. coli and STEC respectively. Besides, 84% (42/50) and 66% (33/50) of chicken meat stalls had at least one contaminated surface with E. coli and STEC respectively. Also, 68.3% (43/63) of strains of E. coli isolated were pathogenic for presenting at least one of the evaluated genes. There were 38 STEC strains and presented stx1 (19.0%, 12/63), stx2 (14.3%, 9/63...

El objetivo del presente trabajo fue determinar si los puestos de venta de carne de pollo son una fuente de contaminación con Escherichia coli shigatoxigénica (STEC) en mercados de abastos. Se tomaron hisopados de la superficie de manos, tablas de picar y mesas de expendio de 50 puestos de expendio de carne de pollo en el distrito de San Juan de Miraflores, Lima, Perú (n=150 muestras). Se realizó aislamiento microbiológico estándar e identificación molecular de los genes stx1, stx2 y eaeA mediante PCR. El 42% (63/150) y 25.3% (38/150) de las muestras fueron positivas a E. coli y STEC, respectivamente. El 84% (42/50) y 66% (33/50) de los puestos de venta poseían al menos una de las superficies contaminadas con E. coli y STEC, respectivamente. El 68.3% (43/63) de las cepas de E. coli aisladas fueron patógenas por presentar al menos un gen evaluado. De estas, 38 cepas fueron STEC y...

The aim of this study was to determine the presence ofListeria monocytogenes in pig carcasses in Lima, Peru. Samples were collected from the the surface of 88 carcasses in a slaughterhouse. Samples were enriched with BLEB medium and PALCAM broth. The pathogen was identified using the in situ hybridization molecular technique. The 13.9 ± 6.1% of carcasses were positive to L. monocytogenes, possibly due to deficiencies in the application and control of good manufacturing practices and standard sanitary operations in the slaughterhouse.

The aim of this study was to determine the presence ofListeria monocytogenes in pig carcasses in Lima, Peru. Samples were collected from the the surface of 88 carcasses in a slaughterhouse. Samples were enriched with BLEB medium and PALCAM broth. The pathogen was identified using the in situ hybridization molecular technique. The 13.9 ± 6.1% of carcasses were positive to L. monocytogenes, possibly due to deficiencies in the application and control of good manufacturing practices and standard sanitary operations in the slaughterhouse.