Se analizaron 17 genomas de Bartonella bacilliformis con el fin de encontrar genes de resistencia a antibióticos a partir de un análisis de homología con otras especies bacterianas. La resistencia intrínseca de esta especie se determinó utilizando las plataformas bioinformáticas PATRIC, GenBank y CARD; se identificó genes asociados a la resistencia a macrólidos, quinolonas, rifampicina, aminoglucósidos y genes de bombas de eflujo relacionadas con la exportación de diversos antibióticos. Además, se hizo la predicción de blancos novedosos para el desarrollo de drogas capaces de combatir el agente causal de la enfermedad de Carrión; esta predicción de blancos drogables se realizó a partir del análisis del proteoma de la cepa B. bacilliformis USM- LMMB07 mediante múltiples capas de datos ómicos (genómica, estructurómica y metabolómica) integrados en la plataforma Target...

Toll-like receptors (TLRs) are transmembrane proteins that are key regulators of innate and adaptive immune responses, particularly TLR4, and they have been identified as potential drug targets for the treatment of disease. Several low-molecular-weight compounds are being considered as new drug targets for various applications, including as immune modulators. Mygalin, a 417 Da synthetic bis-acylpolyamine, is an analog of spermidine that has microbicidal activity. In this study, we investigated the effect of mygalin on the innate immune response based on a virtual screening (VS) and molecular docking analysis. Bone marrow-derived macrophages and the cell lines J774A.1 and RAW 264.7 stimulated with lipopolysaccharide (LPS) were used to confirm the data obtained in silico. Virtual screening and molecular docking suggested that mygalin binds to TLR4 via the protein myeloid differentiation fa...