The Neospora caninum is a protozoan that causes abortion, especially in cattle. Thepresent study was carried out to determine the seroprevalence to Neospora caninum inllamas of the central zone of the country. A total of 175 serum samples of breeding femalellamas of the Agrarian Cooperative Pachacútec were analyzed by the immunofluorescentantibody test (IFAT). The results confirmed the presence of Neospora caninum in llamasof the Central Sierra, but the seroprevalence was low (2.9 ± 2.5 %).

El Neospora caninum es un protozoo implicado como agente causal de abortos y muerte neonatal, principalmente en el ganado bovino. El presente estudio se llevó a cabo para determinar la seroprevalencia a Neospora caninum en llamas de la zona central del país. Se evaluaron 175 sueros de llamas hembras en edad reproductiva, pertenecientes a la empresa Sociedad Agraria de Interés Social (SAIS) Pachacútec, del departamento de Junín, mediante la técnica de inmunofluorescencia indirecta (IFI). Los resultados confirman la presencia del Neospora caninum en llamas de la sierra central, aunque con una seroprevalencia reducida (2.9 ± 2.5%).

Se determinaron los valores hematológicos de paiche, Arapaima gigas, en 120 muestras de sangre colectadas entre mayo de 2009 y enero de 2011. Los peces procedían de dos centros de cultivo piscícola de la provincia de Maynas, Loreto, Perú. Las muestras fueron clasificadas de acuerdo al grupo etario: 10-30, 31-180, 181-365 y >365 días. Se obtuvieron valores de recuento de glóbulos rojos y blancos, hematocrito y hemoglobina, así como el recuento diferencial de leucocitos. Los valores de glóbulos rojos y de hemoglobina se incrementaron con la edad (p<0.05).

Haematological values of paiche, Arapaima gigas, were determined in 120 blood samples collected from May 2009 to January 2011. The fishes were cultivated in two fish farms located in the province of Maynas, Loreto, Peru. Blood samples were classified according to four age groups: 10-30, 31-180, 181-365 and >365 days. Data for erythrocyte and leukocyte counts, haemoglobin, haematocrit and differential leukocyte count were obtained. Red cell count and haemoglobin increased with age (p<0.05).

The present study detected by stool examinations the presence of gastrointestinal parasites in nonhuman primates reared in captivity at the Pucallpa Natural Zoological Park, located in the department of Ucayali - Peru. A total of 72 collective fecal samples were collected from 58 individuals from species Cebus apella (brown capuchins), Saguinus fuscicollis weddelli (saddleback tamarins), Cebus albifrons (white-fronted capuchins), Lagothrix lagotricha (woolly monkey), Ateles paniscus chamek (spider monkey), Saimiri sciureus (squirrel monkey), Aotus nigriceps (black-headed night monkey) and Alouatta seniculus (red howler monkey). Samples were fixed in 10% formalin at 60 °C and analyzed by the direct method, and the Ritchie, Sheather, Sedimentation and Ziehl-Neelsen methods. Gastrointestinal parasites found were Strongyloides cebus (56/72), Paratriotaenia oedipomidatis (8/72), Prosthernorc...

Neosporosis has become a parasitosis of economic importance for dairy cattle due to abortions and neonatal mortality. The study was conducted in 2003 to determine the prevalence of Neospora caninum in cattle of the SAIS Pachacutec farm, located in the highlands of Peru. In total, 347 sera sampleswere collected fromBrown Swiss adult cows. Samples were evaluated through the indirect fluorescent antibody test (IFAT). Results indicated that 12.4 ± 3.5%(45/347) of the animals presented antibodies against N. caninum (corrected prevalence: 13.2 ± 3.5%). Theminimumandmaximumseroprevalence was 2.5 and 19.6% in the 7 sampled herds, without statistical differences. All herds had at least one positive animal. The results confirmed the existence of a moderate prevalence of N. caninum in the studied area.

The aim of this study was to identify gastrointestinal parasites affecting wild cats reared in captivity in four Peruvian zoos through the application of four conventional parasitological techniques (direct method, sedimentation test tube, Sheather method, Ziehl-Nielsen stain). Ten Panthera onca (4 males and 6 females), 8 Puma concolor (4 males and 4 females), 6 Leopardus pardalis (males), 3 Leopardus wiedii (1 male and 2 female) and 2 Leopardus tigrinus (males) were used. In total, 62.1% (18/29) of the individuals were positive to at least one parasite species. Panthera onca and Puma concolor were the most affected cat species (9/10 and 5/5 respectively). Among parasites, the most frequent were the cestode Spirometra mansonoides (38.9%), Toxocara cati (33.3%) and Strongyloides spp (33.3%). No significant association was found between age and sex.

A retrospective analysis of parasitological test results in fecal samples was done.Samples were analyzed at the Parasitology Laboratory, Faculty of Veterinary Medicineand Animal Science, Universidad Peruana Cayetano Heredia between February 2008and March 2012. Out of 476 samples, 119 (25.0%) were positive to at least one parasitespecies. Among positive samples, 71.5, 20.3 and 8.1% corresponded to protozoa,nematodes and cestodes respectively where the most frequent species were Giardiacanis (37/119) and Isospora spp (37/119).

This study evaluated the presence of bacterial and micotic agents in 120 paiches(Arapaima gigas) in two fish farms in the Peruvian Amazon. Findings included sevenbacterial agents: Pseudomonas spp, Bacillus spp, Staphylococcus spp, Streptococcusspp, Escherichia spp, Aeromonas hydrophila and Corynebacterium spp, and one fungus:Saprolegnia spp.

El presente estudio tuvo como objetivo identificar los helmintos gastrointestinales en ratas de Lima Metropolitana, Perú, así como cuantificar la frecuencia de infección, resaltando los resultados de aquellos helmintos de interés zoonótico. Se capturaron 53 Rattus rattus y 20 Rattus norvegicus en cinco zonas urbanas. Los tractos gastrointestinales se procesaron con la técnica de Travassos y las heces se evaluaron por observación directa en el microscopio. El 77.4% de R. rattus y el 100% de R. norvegicus estuvieron infectados con helmintos. En R. rattus se identificaron tres especies de cestodos: Hymenolepis diminuta (39.6%), Rodentolepis fraterna (7.5%) y Raillietina demerariensis (7.5%), seis especies de nematodos: Gongylonema neoplasticum (41.5%), Heterakis spumosa (13.2%), Syphacia muris (11.3%), Strongyloides ratti (15.1%), Aspiculuris tetraptera (11.3%) y Protospirura chancha...

Studies in the Amazonian species Arapaima gigas («paiche») have demonstrated the presence of Dawestrema cycloancistrium monogenean parasite in high parasite loads, mainly in stages between 180 and 365 days of age. D. cycloancistrium adheres to the gills causing a decrease in the degree of oxygenation and stress on the fish predisponing to disease presentation. This study aimed to develop a molecular diagnostic technique for D. cycloancistrium in order to aply it in the management of the fish biodiversity. For this purpose, 200 specimens of D. cycloancistrium were collected, DNA was extracted and quantified, primers were designed and a PCR was developed. The amplification of a sequence of 236 bp of the Dawestrema monogenean was obtained.

The present study detected by stool examinations the presence of gastrointestinal parasites in nonhuman primates reared in captivity at the Pucallpa Natural Zoological Park, located in the department of Ucayali - Peru. A total of 72 collective fecal samples were collected from 58 individuals from species Cebus apella (brown capuchins), Saguinus fuscicollis weddelli (saddleback tamarins), Cebus albifrons (white-fronted capuchins), Lagothrix lagotricha (woolly monkey), Ateles paniscus chamek (spider monkey), Saimiri sciureus (squirrel monkey), Aotus nigriceps (black-headed night monkey) and Alouatta seniculus (red howler monkey). Samples were fixed in 10% formalin at 60 °C and analyzed by the direct method, and the Ritchie, Sheather, Sedimentation and Ziehl-Neelsen methods. Gastrointestinal parasites found were Strongyloides cebus (56/72), Paratriotaenia oedipomidatis (8/72), Prosthernorc...

La neosporosis es una parasitosis que afecta el rendimiento económico del sector ganadero al ocasionar abortos ymortalidad neonatal. El objetivo del presente trabajo fue determinar la prevalencia de Neospora caninum en vacas de la empresa de Sociedad Agrícola de Interés Social (SAIS) Pachacutec, ubicada en el departamento de Junín, en el año 2003. Se evaluaron 347 muestras de suero, recolectadas de vacas Brown Swiss adultas, mediante la prueba de inmunofluorescencia indirecta (IFI). El 12.4 ± 3.5% (45/ 347) de los animales presentaron anticuerpos contra el parásito (prevalencia corregida: 13.2 ± 3.5%). Se observó una frecuencia mínima de 2.5%y una máxima de 19.6% en los siete hatos evaluados, sin encontrar diferencia estadística significativa. En todos los hatos se encontró, por lo menos, un animal positivo a este parásito. Estos resultados confirman la existencia de una pr...

The aim of this study was to identify gastrointestinal parasites affecting wild cats reared in captivity in four Peruvian zoos through the application of four conventional parasitological techniques (direct method, sedimentation test tube, Sheather method, Ziehl-Nielsen stain). Ten Panthera onca (4 males and 6 females), 8 Puma concolor (4 males and 4 females), 6 Leopardus pardalis (males), 3 Leopardus wiedii (1 male and 2 female) and 2 Leopardus tigrinus (males) were used. In total, 62.1% (18/29) of the individuals were positive to at least one parasite species. Panthera onca and Puma concolor were the most affected cat species (9/10 and 5/5 respectively). Among parasites, the most frequent were the cestode Spirometra mansonoides (38.9%), Toxocara cati (33.3%) and Strongyloides spp (33.3%). No significant association was found between age and sex.

Se analizaron retrospectivamente los resultados de exámenes parasitológicos demuestras fecales de caninos en el Laboratorio de Parasitología de la Facultad de Veterinaria y Zootecnia de la Universidad Peruana Cayetano Heredia, Lima, entre febrero de 2008 y marzo de 2012. De un total de 476 muestras, 119 (25.0%) resultaron positivas para al menos un tipo de parásito. De las muestras positivas, el 71.5, 20.3 y 8.1% correspondieron a protozoos, nematodos y cestodos, respectivamente, siendo las especies de mayor frecuencia Giardia canis (37/119) e Isospora spp (37/119).

Se evaluó la presencia de agentes bacterianos y micóticos en 120 paiches (Arapaimagigas) de dos centros de cultivo de la Amazonía peruana. Se reporta la presencia de siete agentes bacterianos: Pseudomonas spp, Bacillus spp, Staphylococcus spp, Streptococcus spp, Escherichia spp, Aeromonas hydrophila y Corynebacterium spp, y de un hongo: Saprolegnia spp.

El presente estudio tuvo como objetivo identificar los helmintos gastrointestinales en ratas de Lima Metropolitana, Perú, así como cuantificar la frecuencia de infección, resaltando los resultados de aquellos helmintos de interés zoonótico. Se capturaron 53 Rattus rattus y 20 Rattus norvegicus en cinco zonas urbanas. Los tractos gastrointestinales se procesaron con la técnica de Travassos y las heces se evaluaron por observación directa en el microscopio. El 77.4% de R. rattus y el 100% de R. norvegicus estuvieron infectados con helmintos. En R. rattus se identificaron tres especies de cestodos: Hymenolepis diminuta (39.6%), Rodentolepis fraterna (7.5%) y Raillietina demerariensis (7.5%), seis especies de nematodos: Gongylonema neoplasticum (41.5%), Heterakis spumosa (13.2%), Syphacia muris (11.3%), Strongyloides ratti (15.1%), Aspiculuris tetraptera (11.3%) y Protospirura chancha...

Estudios realizados en la especie amazónica Arapaima gigas (“paiche”) han demostrado la presencia del parásito monogeneo Dawestrema cycloancistrium en altas cargas parasitarias, principalmente en los estadios comprendidos entre los 180 y 365 días de edad. D. cycloancistrium se adhiere principalmente a las branquias, ocasionando una disminución del grado de oxigenación y estrés en el pez, predisponiéndolo a la presentación de enfermedades. El presente estudio tuvo por objetivo desarrollar una técnica de diagnóstico molecular frente a D. cycloancistrium para su aplicación como medida de control en el manejo de la biodiversidad del paiche. Se recolectaron 200 especímenes de D. cycloancistrium, realizándose la extracción de ADN, cuantificación, diseño de cebadores y elaboración del PCR. Se logró la amplificación de la secuencia 236 pb del monogeneo Dawestrema.

The aim of the study was to identify gastrointestinal parasites in reptiles kept in captivity in Metropolitan Lima. Reptiles in the Huascar Park (Villa El Salvador) and Sinchi Roca Park (Comas), in the zoo of El Buen Pastor School and in the serpentarium of the Instituto Nacional de Salud were sampled. Stoll samples (n=133) were collected from Chelonoidis denticulata (89), Boa constrictor (11), Iguana iguana (6), Trachemys scripta (3), Bothrops atrox (5), Bothrops barnetti (3), Epicrates cenchria (3), Crotalus durissus terrificus (2) and other nine species. The samples were processed by parasitological diagnostic methods: direct, flotation with zinc sulfate, Ritchie and Ziehl Neelsen staining. Results shown Nyctotherus sp (72/89), Balantidium sp (56/89), Sauricola sp (21/89) in C. denticulate; Alaeuris sp (4/6) and Ozolaimus sp (3/6) in I. iguana; oocysts of Cryptosporidium sp (35/89) in...

This study was carried out in 120 paiches (Arapaima gigas) distributed in four age groups (10 to 30, 31 to 180, 181 to 365 days and >1 year of age) from fish farms in the Peruvian Amazon to determine the prevalence and the affected organs. Eight parasite species are reported: monogeneans (Dawestrema cycloancistrium and D. cycloancistrioides), digeneans (Caballerotrema brasiliense), protozoa (Piscinodinium pillulare, Ichthyophthirius multifiliis and Trichodina sp), cestodes (Schizochoerus liguloides), and nematodes (Nilonema senticosum). The prevalence of parasitism was 0, 66.7, 90.0 and 66.7% for the age groups 10-30, 31-180, 181-365 days and >1 year of age, respectively.