Background: Myrciaria dubia “camu-camu” a fruit-bearing shrub native to the Amazon, is known for its high vitamin C content. However, variations in vitamin C biosynthesis and accumulation among different cultivars present challenges for commercial production. This study aimed to establish an efficient in vitro regeneration protocol for the Vitahuayo cultivar through callus induction and somatic embryogenesis. Methods: Stem and leaf explants were cultured on Murashige and Skoog (MS) medium supplemented with 24-dichlorophenoxyacetic acid (24-D) and 6-benzylaminopurine (BAP) to induce callus formation. Somatic embryos were induced using Woody Plant Medium (WPM) supplemented with 2,4-D, thidiazuron (TDZ), indole-3-butyric acid (IBA), naphthaleneacetic acid (NAA) and kinetin (KIN). Result: The best results for callogenesis were obtained with 1 mg/L of 24-D and 0.5 mg/L BAP. Somatic embryo...

The Amazonian shrub Myrciaria dubia (camu-camu) produces vitamin C-rich fruits of growing commercial interest. However, sustainable utilization requires assessment and protection of the genetic diversity of the available germplasm. This study aimed to develop and apply microsatellite markers to assess genetic diversity and construct a core collection of M. dubia germplasm from the Peruvian Amazon. Sixteen polymorphic microsatellite loci were developed using an enrichment approach. The evaluation of 336 genotypes from 43 accessions of the germplasm bank, originating from eight river basins, was conducted using these newly developed markers. Genetic diversity parameters, including observed and expected heterozygosity, were calculated. Analysis of molecular variance (AMOVA) was performed to assess the distribution of genetic variation within and among accessions and river basins. Bayesian c...

The objective of this chapter is to provide scientific information on basic aspects to be taken into account to achieve the successful isolation, biochemical, and molecular characterization and then to evaluate the biotechnological potential of native microalgae of the Peruvian Amazon. Recent investigations reported by our research team has demonstrated that the isolated native microalgae from the Peruvian Amazon have a great potential for the biotechnological production of biodiesel and nutraceuticals. This biotechnological potential was identified thanks to the application of various protocols that were standardized by the authors over the last 5 years. In conclusion, the native microalgae of the Peruvian Amazon have biotechnological potential and are therefore promising for the production of both biodiesel and nutraceuticals. Various species of microalgae were identified, isolated, cu...

The Amazonian shrub Myrciaria dubia (camu-camu) produces vitamin C-rich fruits of growing commercial interest. However, sustainable utilization requires assessment and protection of the genetic diversity of the available germplasm. This study aimed to develop and apply microsatellite markers to assess genetic diversity and construct a core collection of M. dubia germplasm from the Peruvian Amazon. Sixteen polymorphic microsatellite loci were developed using an enrichment approach. The evaluation of 336 genotypes from 43 accessions of the germplasm bank, originating from eight river basins, was conducted using these newly developed markers. Genetic diversity parameters, including observed and expected heterozygosity, were calculated. Analysis of molecular variance (AMOVA) was performed to assess the distribution of genetic variation within and among accessions and river basins. Bayesian c...