1
tesis de grado
Publicado 2019
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Aniba rosaeodora Ducke “palo de rosa” es un árbol forestal amazónico en peligro de extinción, que posee “Linalool” un compuesto aromático con alto valor económico en la industria de la perfumería fina. Para evaluar la diversidad genética de esta especie se colectaron 90 genotipos provenientes de 8 poblaciones y se emplearon 11 marcadores ISSR. El ADN aislado fue de óptima calidad, así lo demuestra la prueba electroforética y espectrofotométrica. A partir de ello, se amplificaron 11 marcadores ISSR, obteniendo 375 bandas polimórficas y un PIC promedio de 0,774. El promedio del número efectivo de alelos (Ne), la diversidad genética de Nei (He) y el índice informativo de Shannon (I) fue 1,485, 0,294 y 0,453 respectivamente. El resultado del AMOVA, reveló una alta variación genética dentro de las poblaciones (88%) y baja entre las poblaciones (12%). La estructura ge...
2
artículo
Publicado 2024
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The Amazonian shrub Myrciaria dubia (camu-camu) produces vitamin C-rich fruits of growing commercial interest. However, sustainable utilization requires assessment and protection of the genetic diversity of the available germplasm. This study aimed to develop and apply microsatellite markers to assess genetic diversity and construct a core collection of M. dubia germplasm from the Peruvian Amazon. Sixteen polymorphic microsatellite loci were developed using an enrichment approach. The evaluation of 336 genotypes from 43 accessions of the germplasm bank, originating from eight river basins, was conducted using these newly developed markers. Genetic diversity parameters, including observed and expected heterozygosity, were calculated. Analysis of molecular variance (AMOVA) was performed to assess the distribution of genetic variation within and among accessions and river basins. Bayesian c...
3
artículo
Publicado 2021
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Research studies on conservative genetics of endangered plants are very important to establish the management plans for the conservation of biodiversity. Rosewood is an evergreen tree of the Amazon region and its essential oil has great acceptance in the medical and cosmetic industry. The present study aimed to explore the genetic diversity and population structure of 90 rosewood accessions collected from eight localities of Peruvian Amazon territory through DArTseq markers. A total of 7485 informative markers resulted from genotyping by sequencing (GBS) analysis were used for the molecular characterization of rosewood germplasm. Mean values of various calculated diversity parameters like observed number of alleles (1.962), the effective number of alleles (1.669), unbiased expected heterozygosity (0.411), and percent polymorphism (93.51%) over the entire germplasm showed the existence of...