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1
artículo
Publicado 2015
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In 65 normal pregnant protein iodine numbers are determined via the technique of chloric acid. In the first trimester of pregnancy is an average value of 8.82 ug%; in the second quarter 10 ug% in the third quarter and 11.99 ug%; These elevations Iodine protein in normal pregnancy as due to increased estrogen, in turn, cause an increase in transport capacity of the thyroid hormone binding proteins, not increasing the free thyroxine are interpreted, which explains the absence of clinical symptoms of hyperthyroidism.
2
artículo
Publicado 2015
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In 65 normal pregnant protein iodine numbers are determined via the technique of chloric acid. In the first trimester of pregnancy is an average value of 8.82 ug%; in the second quarter 10 ug% in the third quarter and 11.99 ug%; These elevations Iodine protein in normal pregnancy as due to increased estrogen, in turn, cause an increase in transport capacity of the thyroid hormone binding proteins, not increasing the free thyroxine are interpreted, which explains the absence of clinical symptoms of hyperthyroidism.
3
artículo
Publicado 2022
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The aim of this study was to evaluate the sedative effect of three products: valerian extract (Valeriana officinalis), benzocaine and tricaine methanesulfonate (MS-222) on stress levels produced by a simulated transport model in tilapia fingerlings. In a first part, the sedative dose of the products was determined based on the clinical characteristics that indicate depression of the central nervous system. In the second part, sedation and subsequent simulated transport for 3 hours were performed. The fish were euthanized, and blood samples were taken from the tail vein to determine cortisol (ELISA) and glucose (portable glucometer) levels, haematological parameters and mortality. The optimal doses of sedation were 200, 35 and 75 mg/l, respectively, for valerian extract, benzocaine and MS-222. After 3 h of simulated transport, cortisol and glucose values were similar or higher compared to...
4
artículo
Most Vibrio species identification methods take many days to deliver results, therefore it is important that research can be done on rapid and accurate detection and identification techniques. MALDI-TOF MS has proven to be a simple tool to identify and differentiate pathogens. The objective of this research was to recognize the available bibliographic information on the identification of Vibrio cholerae species non 01 non 139 of environmental origin by MALDI TOF MS. The Vibrio genera includes more than thirty species found in aquatic environments, some species cause diseases in both marine and human species. One of these organisms is V. cholerae, one of the most important species due to the production of cholera toxin (CT) which interrupts the transport of ions in the cells of the intestinal epithelium. The number of case reports involving V. cholerae no 01 no 139 in extraintestinal infe...
5
tesis de maestría
Publicado 2025
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La neurocisticercosis es una enfermedad parasitaria del SNC que causa epilepsia adquirida y es generada por la forma larvaria del cestodo Taenia solium. Es de curso crónico y en pacientes sintomáticos se caracteriza por convulsiones y epilepsia. Estudios en ratas de laboratorio con NCC, reportan daño axonal caracterizado por el acúmulo de proteínas (neurofilamento, APP) en forma de hinchazones axonales, que podría estar relacionado con deterioro en el transporte axonal. Objetivo: Evaluar el patrón de inmunorreactividad de las proteínas motoras kinesina y dineína como marcadores de daño en el transporte axonal en tejido cerebral de ratas Holtzman con NCC tratadas y no tratadas con antiparasitarios; y su asociación con el daño axonal. Metodología: Se utilizó tejido cerebral de ratas con NCC tratadas y no tratadas con antiparasitarios y ratas sanas sacrificadas a diferentes ti...
6
Publicado 2019
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El objetivo de este trabajo de investigación fue las características estructurales de la célula en general son la individualidad, la sistematicidad y la tridimensionalidad. Las células contienen citosol y material genético y poseen enzimas y diversas proteínas. La membrana celular es una estructura formada por una bicapa lipídica que envuelve y delimita a la célula en su conjunto. El núcleo celular es un orgánulo membranoso en forma de esfera, localizado por lo general en la zona central de las células eucariotas. Contiene la mayor parte de la carga genética celular bajo la forma de moléculas muy largas de ADN. Estructuralmente, el núcleo celular consta de envoltura nuclear, poros nucleares, nucléolo, lámina nuclear, cromatina y cromosoma, nucleoplasma, otros cuerpos subnucleares (cuerpos de Cajal, PIKA, cuerpos PML, paraspeckles, speckles, cuerpos de escisión, cuerpos D...
7
tesis de grado
Publicado 2021
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Taenia solium develops into a sexually mature flatworm exclusively in the human gut; its eggs are excreted with the host´s feces, contaminating water, soil, and food. The evagination of the scolex from the ingested larval cyst and the consequent cellular proliferation are critical processes for the parasite´s development and growth into the adult form. Changes in the metabolism and glucose transport in proliferating tissues and during regeneration have been reported for other flatworm species. In T. solium, glucose transporters TGTP1 and TGTP2 have been identified, as well as a hypothetical SGLT1-like sodium-glucose cotransporter in an aberrant, hyperproliferative larval form. However, it is not known if these transporters play a significant role in the parasite´s metamorphosis. We found variations at the mRNA level of glucose transporters using RT-qPCR on T. solium cysts obtained fro...
8
tesis de maestría
Publicado 2022
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Las vacunas más importantes en la actualidad las constituyen principalmente proteínas recombinantes, las cuales se caracterizan por una rápida degradación y corto tiempo de circulación, por lo que su exposición al sistema inmune se limita a períodos breves. En ese sentido, múltiples dosis y refuerzos son necesarios para compensar esta limitación. Más recientemente se vienen probando con éxito las denominadas vacunas peptídicas, péptidos correspondientes a epítopes inmunogénicos/protectivos que se sintetizan químicamente y son administrados en varias dosis. Los epítopes son la porción clave de una proteína responsable de la inducción de inmunidad y protección. El uso de múltiples epítopes constituyen las denominadas vacunas multiepitópicas, las cuales fundamentalmente consisten de arreglos lineales de epítopes inmunogénico/protectivos unidos químicamente a la su...
9
artículo
Iron is essential for health; its deficiency and excess are harmful. Our bodies have a high capacity to store and reuse iron so that its requirement is small (1-2 mg absorbed/day). Hepcidin, a hormone produced in the liver, has an important role in this element’s homeostasis by blocking its transport protein, inhibiting its absorption in the duodenum and its release from the iron stores. During pregnancy, there is a new iron requirement for the placenta and fetus. This causes an increase in erythropoiesis; however, hemoglobin concentration decreases due to the greater vascular expansion. This results in hemodilution, which is evident starting the second trimester and returns to pre-gestational values at the end of the third trimester. Maternal iron deficiency anemia becomes a public health problem when it is moderate (7-14,5 g/dL) during pregnancy adversely affect the mother and neonat...
10
artículo
Publicado 2019
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Se analizaron las características de una cepa de Bacillus amyloliquefaciens aislada del intestino de Arapaima gigas, seleccionada por su capacidad para generar inhibición de múltiples bacterias patógenas de peces, mediante el uso de herramientas moleculares como PCR y espectrometría de masas - MALDI TOF/TOF. Los resultados mostraron a través de PCR que esta cepa cuenta con genes claves para la generación de péptidos antimicrobianos como bmyB (bacillomycin L synthetase B), fenD (fengycin sintetasa), srfAA (subunidad 1 surfactin sintetasa), bacA (proteína de biosíntesis de bacilysin) e iturin (iturin A). Además, mediante el análisis por espectrometría de masas se detectaron bacteriocinas (plipastatin, gramicidin, fengycin, surfactin), proteínas de fijación al intestino (like-enolase), proteinas transportadoras de péptidos antimicrobianos (ABC-transporters) y proteínas de e...
11
artículo
Publicado 2019
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The characteristics of a strain of Bacillus amyloliquefaciens isolated from the intestine of Arapaima gigas, selected for its ability to generate inhibition of multiple pathogenic bacteria of fishes were analyzed by using molecular tools such as PCR and mass spectrometry - MALDI TOF / TOFM. The results showed through PCR that this strain has key genes for the generation of antimicrobial peptides such as bmyB (bacillomycin L synthetase B), fenD (fengycin synthetase), srfAA (subunit 1 surfactin synthetase), bacA (bacilysin biosynthesis protein) and iturin (iturin A). In addition, by mass spectrometry analysis were detected bacteriocins (plipastatin, gramicidin, fengycin, surfactin), intestine binding proteins (like-enolase), antimicrobial peptide transport proteins (ABC-transporters) and immune system stimulation proteins like flagelin. Collagenase, chitinases and xylose-isomerases protein...
12
artículo
Publicado 2013
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Iron is essential for health but in excess may be harmful. The living organism has ability to store and re-use the iron body and as such its requirement is small (1-2 mg absorbed/day). Hepcidin, a hormone produced in the liver, has an important role in iron homeostasis by blocking the iron transport protein and inhibiting iron absorption. Maternal anemia due to iron deficiency is a public health problem when it is moderate (7-<9 g/dL) and severe (Hb<7 g/dL) since it increases the risk of maternal and neonatal morbi-mortality. Similarly, high levels of hemoglobin during pregnancy affect the mother and newborn. In Peru, there is a National Program to supplement with iron to all pregnant women anemic or not. Scientific evidence shows that hemoglobin values between 9-10.5 g/dL are optimal for best fetal growth and that treatment with iron supplementation to non anemic women increases levels ...
13
artículo
Publicado 2013
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Iron is essential for health but in excess may be harmful. The living organism has ability to store and re-use the iron body and as such its requirement is small (1-2 mg absorbed/day). Hepcidin, a hormone produced in the liver, has an important role in iron homeostasis by blocking the iron transport protein and inhibiting iron absorption. Maternal anemia due to iron deficiency is a public health problem when it is moderate (7-<9 g/dL) and severe (Hb<7 g/dL) since it increases the risk of maternal and neonatal morbi-mortality. Similarly, high levels of hemoglobin during pregnancy affect the mother and newborn. In Peru, there is a National Program to supplement with iron to all pregnant women anemic or not. Scientific evidence shows that hemoglobin values between 9-10.5 g/dL are optimal for best fetal growth and that treatment with iron supplementation to non anemic women increases l...
14
tesis de grado
The fruit of the Bixa orellana L. (achiote) is highly appreciated by people as a member for the preparation of their food, and by entrepreneurs as a supplier agent of natural color very appreciated now, but like all fruits its production it occurs by seasons causing that not all the production is used but only a part of it wasting the rest. The main objective of this work was to carry out an experimental study of the convective behavior during the drying of Bixa orellana L. (achiote) in a SDBASIC SPRAY DRYER spray dryer located in the Food Engineering Laboratory of the Pilot Plant of the Faculty of Food Industries of the National University of the Peruvian Amazon. To obtain the powder of Bixa orellana L. (achiote) a factorial statistical arrangement 23 was proposed, represented by the equation: 0 1 1 2 2 3 3 4 1 2 5 1 3 6 2 3 7 2 3 Y ? ? ?? X ?? X ?? X ?? X X ?? X X ?? X X ?? X X with th...
15
artículo
Publicado 2021
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Potato virus X (PVX) occurs worldwide and causes an important potato disease. Complete PVX genomes were obtained from 326 new isolates from Peru, which is within the potato crop′s main domestication center, 10 from historical PVX isolates from the Andes (Bolivia, Peru) or Europe (UK), and three from Africa (Burundi). Concatenated open reading frames (ORFs) from these genomes plus 49 published genomic sequences were analyzed. Only 18 of them were recombinants, 17 of them Peruvian. A phylogeny of the non-recombinant sequences found two major (I, II) and five minor (I-1, I-2, II-1, II-2, II-3) phylogroups, which included 12 statistically supported clusters. Analysis of 488 coat protein (CP) gene sequences, including 128 published previously, gave a completely congruent phylogeny. Among the minor phylogroups, I-2 and II-3 only contained Andean isolates, I-1 and II-2 were of both Andean and...
16
tesis de grado
Publicado 1996
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This work is the development of coconut flakes Jl salty sweets using the hybrid - 121 (cocos nucifera l.) variety, its subsequent storage and transport. The samples obtained have the following characteristics: sweet pancakes; humidity 0.79%, fat 52.70%, proteins 6.10%, carbohydrates 35. 695), mineral 0.85%, fiber 3.87%; While the flakes salty presented the following composition: moisture or. HO ~ ~, fat 62.83%, proteins 6,78%, carbohydrates 22.27%, minerals 2. 30 ~ t, fiber 4. 92% reducing sugars no• presents in both samples. Was determined to do this the optimal parameters of flow of processing; control during and after the time of storage. The stages of the process involves the following operations: selection, pre-cooking, shell, mondado, washed, bleached, grated, pressed, first drying, preparation of solutions, mixing, second dried, cooled, packaged, stored and transport. In terms o...
17
tesis de grado
Publicado 2007
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Son ampliamente conocidas las ventajas de los productos vegetales y frutas secas, ya que, al reducir su contenido de humedad, disminuye notablemente la actividad microbiológica y enzimática, además, disminuye los costos de almacenamiento y transporte. El presente trabajo, tuvo por objetivo obtener rodajas de piña (Ananas comosus) seca, variedad Cayena lisa, utilizando procesos combinados de deshidratación osmótica y secado; fueron utilizados como osmodeshidratantes sacarosa y etanol. La composición proximal de la piña fresca con humedad, proteínas, grasa, cenizas, fibra y carbohidratos totales de: 87.64 %, 0.14 %, 0.12%, 0.30 %, 0.39 % y 11.38 % respectivamente; el contenido de ácido ascórbico fue de 26.85 mg/100g- previamente a la deshidratación osmótica se verifico que las rodajas de piña de 1 cm de espesor, estén en condiciones óptimas de frescura, se les retiro el cor...
18
tesis de maestría
Publicado 2019
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Universidad Nacional Agraria La Molina. Escuela de Posgrado. Maestría en Nutrición
19
artículo
Publicado 2023
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The empirical use of alpaca rennet in the production of artisanal cheeses with cow’s milk is widespread in the high Andean communities of Perú and Bolivia, and has not been scientifically reported. The objective of the present study was to characterize alpaca rennet and evaluate its coagulating activity. Ready-to-use alpaca curds were collected directly from dairies in four neighborhoods of Viscapalca (Huancavelica-Perú, 4003 masl), which were transported under refrigeration and kept frozen at -20°C. The Crude Enzymatic Extract (CEE) was obtained by centrifugation at 560 x g for 10 min at 4°C, from which proteins were determined by Bradford, SDS-PAGE electrophoretic profile, coagulation time and proteolytic activity. The traditional management of alpaca rennet is also described. CEE was found to contain 29.80 to 52.91 µg/ml of protein. The SDS-PAGE electrophoretic profile revealed...
20
artículo
Publicado 2023
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The empirical use of alpaca rennet in the production of artisanal cheeses with cow’s milk is widespread in the high Andean communities of Perú and Bolivia, and has not been scientifically reported. The objective of the present study was to characterize alpaca rennet and evaluate its coagulating activity. Ready-to-use alpaca curds were collected directly from dairies in four neighborhoods of Viscapalca (Huancavelica-Perú, 4003 masl), which were transported under refrigeration and kept frozen at -20°C. The Crude Enzymatic Extract (CEE) was obtained by centrifugation at 560 x g for 10 min at 4°C, from which proteins were determined by Bradford, SDS-PAGE electrophoretic profile, coagulation time and proteolytic activity. The traditional management of alpaca rennet is also described. CEE was found to contain 29.80 to 52.91 µg/ml of protein. The SDS-PAGE electrophoretic profile revealed...
