A rapid detection of Campylobacter jejuni in chickens by nested polymerase chain reaction

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In order to standardize a rapid method for detecting C. jejuni in chickens, it was taken cloacal swabs from 50 chickens of seven-week lifetime belonging to several markets of Lima's city. DNA was extracted by using organic solvents and the presence of C. jejuni was detected by means of Nested-P...

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Detalles Bibliográficos
Autores: Jiménez, Karim L., Zavaleta, Amparo I., lzaguirre, Victor, Koga, Ysabel, Soto, Javier
Formato: artículo
Fecha de Publicación:2007
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revistas - Universidad Nacional Mayor de San Marcos
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/5634
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/farma/article/view/5634
Nivel de acceso:acceso abierto
Materia:C. jejuni
Nested-PCR
16S ribosomal genes
hipO gen
chickens
C. jejun
PCR anidada
genes ribosómicos 16S
gen hipo
pollos
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spelling A rapid detection of Campylobacter jejuni in chickens by nested polymerase chain reactionDetección rápida de Campylobacter jejuni en pollos mediante la reaccion en cadena de la Polimerasa AnidadaJiménez, Karim L.Zavaleta, Amparo I.lzaguirre, VictorKoga, YsabelSoto, JavierC. jejuniNested-PCR16S ribosomal geneshipO genchickensC. jejunPCR anidadagenes ribosómicos 16Sgen hipopollosIn order to standardize a rapid method for detecting C. jejuni in chickens, it was taken cloacal swabs from 50 chickens of seven-week lifetime belonging to several markets of Lima's city. DNA was extracted by using organic solvents and the presence of C. jejuni was detected by means of Nested-PCR using specific primers for 16S ribosomal and hipO genes. In parallel with this rapid DNA-based approach, it was isolated C. jejuni by conventional filtration bacteriological methods and selective culture mediums. The rapid and conventional methods used in this study, detected C jejuni in 48/50 (96%) and 29/50 (58%) respectively. These findings indicate than the rapid method has a higher sensitivity lhan the conventional one.Con el objetivo de estandarizar un método rápido para la detección de C. jejuni en pollos se tomaron hisopados cloacales de 50 pollos de 7 semanas de edad en diferentes mercados del distrito de Lima. Se extrajo el ADN por solventes orgánicos y se detectó la presencia de C. jejuni mediante la reacción en cadena de la polimerasa anidada utilizando cebadores específicos para los genes ribosómicos 16S y gen hipO. Paralelamente, utilizando métodos de filtración bacteriológicos convencionales se aisló C. jejuni en medios selectivos. Los métodos rápido y convencional permitieron detectar C. jejuni en el 48/50 (96%) Y 29/50 (58%) de las muestras analizadas, respectivamente. Los hallazgos de este estudio indican que el método rápido tiene mayor sensibilidad que el convencional.Universidad Nacional Mayor de San Marcos, Facultad de Farmacia y Bioquímica2007-06-18info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/farma/article/view/563410.15381/ci.v10i1.5634Ciencia e Investigación; Vol. 10 Núm. 1 (2007); 7-11Ciencia e Investigación; Vol. 10 No. 1 (2007); 7-111609-90441561-0861reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/farma/article/view/5634/5677Derechos de autor 2007 Karim L. Jiménez, Amparo I. Zavaleta, Victor lzaguirre, Ysabel Koga, Javier Sotohttps://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/56342020-04-26T13:18:39Z
dc.title.none.fl_str_mv A rapid detection of Campylobacter jejuni in chickens by nested polymerase chain reaction
Detección rápida de Campylobacter jejuni en pollos mediante la reaccion en cadena de la Polimerasa Anidada
title A rapid detection of Campylobacter jejuni in chickens by nested polymerase chain reaction
spellingShingle A rapid detection of Campylobacter jejuni in chickens by nested polymerase chain reaction
Jiménez, Karim L.
C. jejuni
Nested-PCR
16S ribosomal genes
hipO gen
chickens
C. jejun
PCR anidada
genes ribosómicos 16S
gen hipo
pollos
title_short A rapid detection of Campylobacter jejuni in chickens by nested polymerase chain reaction
title_full A rapid detection of Campylobacter jejuni in chickens by nested polymerase chain reaction
title_fullStr A rapid detection of Campylobacter jejuni in chickens by nested polymerase chain reaction
title_full_unstemmed A rapid detection of Campylobacter jejuni in chickens by nested polymerase chain reaction
title_sort A rapid detection of Campylobacter jejuni in chickens by nested polymerase chain reaction
dc.creator.none.fl_str_mv Jiménez, Karim L.
Zavaleta, Amparo I.
lzaguirre, Victor
Koga, Ysabel
Soto, Javier
author Jiménez, Karim L.
author_facet Jiménez, Karim L.
Zavaleta, Amparo I.
lzaguirre, Victor
Koga, Ysabel
Soto, Javier
author_role author
author2 Zavaleta, Amparo I.
lzaguirre, Victor
Koga, Ysabel
Soto, Javier
author2_role author
author
author
author
dc.subject.none.fl_str_mv C. jejuni
Nested-PCR
16S ribosomal genes
hipO gen
chickens
C. jejun
PCR anidada
genes ribosómicos 16S
gen hipo
pollos
topic C. jejuni
Nested-PCR
16S ribosomal genes
hipO gen
chickens
C. jejun
PCR anidada
genes ribosómicos 16S
gen hipo
pollos
description In order to standardize a rapid method for detecting C. jejuni in chickens, it was taken cloacal swabs from 50 chickens of seven-week lifetime belonging to several markets of Lima's city. DNA was extracted by using organic solvents and the presence of C. jejuni was detected by means of Nested-PCR using specific primers for 16S ribosomal and hipO genes. In parallel with this rapid DNA-based approach, it was isolated C. jejuni by conventional filtration bacteriological methods and selective culture mediums. The rapid and conventional methods used in this study, detected C jejuni in 48/50 (96%) and 29/50 (58%) respectively. These findings indicate than the rapid method has a higher sensitivity lhan the conventional one.
publishDate 2007
dc.date.none.fl_str_mv 2007-06-18
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/farma/article/view/5634
10.15381/ci.v10i1.5634
url https://revistasinvestigacion.unmsm.edu.pe/index.php/farma/article/view/5634
identifier_str_mv 10.15381/ci.v10i1.5634
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/farma/article/view/5634/5677
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Farmacia y Bioquímica
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Farmacia y Bioquímica
dc.source.none.fl_str_mv Ciencia e Investigación; Vol. 10 Núm. 1 (2007); 7-11
Ciencia e Investigación; Vol. 10 No. 1 (2007); 7-11
1609-9044
1561-0861
reponame:Revistas - Universidad Nacional Mayor de San Marcos
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
reponame_str Revistas - Universidad Nacional Mayor de San Marcos
collection Revistas - Universidad Nacional Mayor de San Marcos
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 13.905282
A rapid detection of Campylobacter jejuni in chickens by nested polymerase chain reaction
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