Improved methodological tools to hasten antimalarial drug discovery remain of interest, especially when considering natural products as a source of drug candidates. We propose a biodereplication method combining the classical dereplication approach with the early detection of potential antiplasmodial compounds in crude extracts. Heme binding is used as a surrogate of the antiplasmodial activity and is monitored by mass spectrometry in a biomimetic assay. Molecular networking and automated annotation of targeted mass through data mining were followed by mass-guided compound isolation by taking advantage of the versatility and finely tunable selectivity offered by centrifugal partition chromatography. This biodereplication workflow was applied to an ethanolic extract of the Amazonian medicinal plant Piper coruscans Kunth (Piperaceae) showing an IC50 of 1.36 ug/mL on the 3D7 Plasmodium falc...

Ethnopharmacological relevance: In the Peruvian Amazon as in the tropical countries of South America, the use of medicinal Piper species (cordoncillos) is common practice, particularly against symptoms of infection by protozoal parasites. However, there is few documented information about the practical aspects of their use and few scientific validation. The starting point of this work was a set of interviews of people living in six rural communities from the Peruvian Amazon (Alto Amazonas Province) about their uses of plants from Piper genus: one community of Amerindian native people (Shawi community) and five communities of mestizos. Infections caused by parasitic protozoa take a huge toll on public health in the Amazonian communities, who partly fight it using traditional remedies. Validation of these traditional practices contributes to public health care efficiency and may help to id...

Se realizó la caracterización química de las semillas de nueve accesiones de sacha inchi Plukenetia volubilis L. del “Centro de Investigaciones de Pucayacu” (Banco de germoplasma - Instituto de Investigaciones de la Amazonía Peruana) en Tarapoto, San Martín. Esta caracterización fue basada en análisis centesimales, análisis fisicoquímicos de aceites, determinación de minerales, caracterización de ácidos grasos e identificación de aminoácidos esenciales. Se determinó que las mejores accesiones en rendimiento de aceite fueron las accesiones 4 y 2 con 20.12% y 20.23% respectivamente. En cuanto a minerales las mejores accesiones fueron (en miligramos por 100g de muestra seca) el 4 (K=892.93; Na=238.02; Zn=16.43) y el 5 (K=863.65; Zn=15.95; Mg=340.15). En tanto que en ácidos grasos los mayores valores de ù-3, fueron encontrados en la accesión 18 (47.91%) y ù-6 en...

Se realizó la caracterización química de las semillas de nueve accesiones de sacha inchi Plukenetia volubilis L. del “Centro de Investigaciones de Pucayacu” (Banco de germoplasma - Instituto de Investigaciones de la Amazonía Peruana) en Tarapoto, San Martín. Esta caracterización fue basada en análisis centesimales, análisis fisicoquímicos de aceites, determinación de minerales, caracterización de ácidos grasos e identificación de aminoácidos esenciales. Se determinó que las mejores accesiones en rendimiento de aceite fueron las accesiones 4 y 2 con 20.12% y 20.23% respectivamente. En cuanto a minerales las mejores accesiones fueron (en miligramos por 100g de muestra seca) el 4 (K=892.93; Na=238.02; Zn=16.43) y el 5 (K=863.65; Zn=15.95; Mg=340.15). En tanto que en ácidos grasos los mayores valores de ù-3, fueron encontrados en la accesión 18 (47.91%) y ù-6 en...

We explored the concentration patterns of the bioactive metabolite plumericin produced by Himatanthus tarapotensis (Apocynaceae) under different edaphic conditions and variations in rainfall intensity, as well as its potential role in the chemical defense against insect herbivores. Values of plumericin concentration from leaves were obtained by High-Performance Liquid Chromatography, and evaluated as a function of differences in soil types, variation of precipitation, and variation of the abundance of insect herbivores, using first a Repeated Measures Correlation (rmcorr) and then a Generalized Linear Mixed Model (GLMM) analysis. Plumericin concentration is highly variable among plants, but with a significantly higher concentration in plants growing on clay soil compared to that of the white-sand soil habitat (p < 0.001). Plumericin concentration is not affected by precipitation. The cat...

Ethnopharmacological relevance: Fungal and bacterial infections remain a major problem worldwide, requiring the development of effective therapeutic strategies. Solanum mammosum L. (Solanaceae) (“teta de vaca”) is used in traditional medicine in Peru to treat fungal infections and respiratory disorders via topical application. However, the mechanism of action remains unknown, particularly in light of its chemical composition. Materials and methods: The antifungal activity of TDV was determined against Trichophyton mentagrophytes and Candida albicans using bioautography-TLC-HRMS to rapidly identify the active compounds. Then, the minimum inhibitory concentration (MIC) of the fruit crude extract and the active compound was determined to precisely evaluate the antifungal activity. Additionally, the effects of the most active compound on the formation of Pseudomonas aeruginosa biofilms a...

This research was funded by the French National Alliance for Life Sciences and Health, grant number ENV201408. S.B. has received funding from the European Union's Horizon 2020 Framework program under the Marie Sklodowska-Curie Actions, agreement number 823935. P.V.O. was supported by a postdoctoral fellowship from the French National Research Institute for Sustainable Development (IRD) (04077858). JPC was a recipient of a doctoral fellowship from the Peruvian National Council for Science and Technology (212-2015-FONDECYT).